Identification and Induction of hsp70 Gene by Heat Shock and Cadmium Exposure in Carp
A member of the multi-gene family, encoding 70 kD stress proteins, was identified from the common carp (Cyprinus carpio). Homologies, observed at both nucleic acid and amino acid levels, and also the intronless structure of this gene, strongly suggest that it corresponds to a heat-inducible hsp70 gene in carp. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal hsp70 mRNA levels and to follow the inducer-specific expression of this gene in different tissues during in vivo studies. Carp hsp70 mRNA is not detectable in the brain and muscle, and its concentration is around the limit of detection in the kidney and liver of unstressed animals. The expression of hsp70 is induced by elevated temperature and it responds to Cd treatment in a tissue and time-dependent manner.
KeywordsCadmium fish heat shock hsp70 expression liver muscle
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