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Acta Biologica Hungarica

, Volume 54, Issue 1, pp 1–14 | Cite as

The Biology of the Post-Genomic ERA: The Proteomics

Review
  • A. Mádi
  • Tünde Pusztahelyi
  • Mária Punyiczki
  • L. FésüsEmail author
Open Access
Article

Abstract

The complete identification of coding sequences in a number of species has led to announce the beginning of the post-genomic era, new tools have become available to study complex phenomena in biological systems. Rapid advances in genomic sequencing and bioinformatics have established the field of genomics to investigate thousands of genes’ activity through mRNA display. However, recent studies have demonstrated a lack of correlation between the transcriptional profiles and the actual protein levels in cells, so investigation of the expressed part of the genome is also required to link genomic data to biological function. It is possible that evolutional development occurred by increasing complexity of regulation processes at the level of RNA and protein molecules instead of simple increase in gene number, so investigation of proteins and protein complexes became important fields of our post-genomic era. Highresolution two-dimensional gels combined with sensitive mass spectrometry can reveal virtually all proteins present in cells opening new insights into functions of cells, tissues and whole organisms.

Keywords

Post-genomic era proteomics Caenorhabditis elegans 

Abbreviations

2-DE

two-dimensional gel-electrophoresis

MS

mass spectrometry

IPG

immobilized pH gradient

SDS

sodium dodecyl sulphate

PAGE

polyacrylamide gel-electrophoresis

TCA

trichlororacetic acid

CBB

Coomassie Brillant Blue

CDD

charge-coupled device

MALDI-TOF

matrix-assisted laser desorption ionization-time of flight

ESI

electrospray ionization

PCD

programmed cell death

DSP

disease-specific protein

LCM

laser capture microdissection

SPR-BIA

surface plasmon resonance-biomolecular interaction analysis

Notes

Acknowledgement

This work was supported by OTKA F030338.

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© Akadémiai Kiadó, Budapest 2003

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Mádi
    • 1
  • Tünde Pusztahelyi
    • 2
  • Mária Punyiczki
    • 3
  • L. Fésüs
    • 1
    • 3
    Email author
  1. 1.Signal Transduction and Apoptosis Research Group of the Hungarian Academy of SciencesUniversity of DebrecenDebrecenHungary
  2. 2.Department of Microbiology and BiotechnologyUniversity of DebrecenDebrecenHungary
  3. 3.Department of Biochemistry and Molecular BiologyUniversity of DebrecenDebrecenHungary

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