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Acta Biologica Hungarica

, Volume 53, Issue 4, pp 495–498 | Cite as

DNA Profiling by Detection of Repetitive Nucleotide Sequences on Human Chromosome 6

  • L. Keresztury
  • A. Lászik
  • Hargita Hegyesi
  • A. FalusEmail author
Article

Abstract

Genetic/genomic polymorphism, i.e. variations in DNA sequences are ideally assayed by direct nucleotide sequencing of a gene region or other homologous segment of the genome. An easier and cheaper approach, however, if the variants are analyzed by hybridization technology using restriction fragment length polymorphisms (RFLPs) or by detection of the number of tandem repeats (VNTR) of small DNA segments, the “minisatellites”. In this study we describe results of the DNA analysis of repetitive sequences of human 6th chromosome by the application of a chemiluminescent labeled probes. The allele frequency distribution of polymorphic DNA sequences has been determined in unrelated individuals. The isolated genomic DNA was cut with Pst I restriction enzyme, size fractionated on agarose gel and hybridized with a chemiluminescent labeled D6 S132 probe. At this locus the Pst I cleaved DNA fragments are ranging from 1841 to 6098 base pairs (bp).

Specific genetic pattern was characterized by more frequent fragments (3313 and 3884 bp), and the rarely occurring ones (clustered between 1841–2595 and 5227–6098 bp). Our study provides a further possibility for characterization of individual genomic patterns.

Keywords

Gene technology forensic medicine RFLP VNTR chemiluminescent probe 

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Copyright information

© Akadémiai Kiadó, Budapest 2002

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • L. Keresztury
    • 1
  • A. Lászik
    • 2
  • Hargita Hegyesi
    • 3
  • A. Falus
    • 3
    • 4
    Email author
  1. 1.National Institute of Haematology and ImmunologyBudapestHungary
  2. 2.Department of Forensic MedicineSemmelweis University of MedicineBudapestHungary
  3. 3.Department of Genetics, Cell and ImmunobiologySemmelweis University of MedicineBudapestHungary
  4. 4.Molecular Immunology Research GroupHungarian Academy of SciencesBudapestHungary

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