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Acta Biologica Hungarica

, Volume 52, Issue 4, pp 411–416 | Cite as

Tight Junctional Changes Upon Microwave and X-Ray Irradiation

  • Z. PálfiaEmail author
  • Z. Somosy
  • G. Réz
Article

Abstract

Tight junctions (zonulae occludentes, ZO) are cellularly regulated dynamic structures sensitive to environmental stress agents including ionizing radiation. Radiation induced pathological alterations of the small intestine (gastrointestinal radiation syndrome) are related to altered ZO-mediated paracellular transport. We carried out a quantitative morphological evaluation of the murine jejunal epithelial tight junctional structure in freeze fracture replicas as changed upon whole body X-ray irradiation and low energy microwave exposition. X-ray treatment (4 Gy, 1, 24 h) brought about a partial dearrangement of the ZO strand network which regenerated only partially by 24 h. This observation is in line with data on paracellular permeability increases and ZO-bound calcium drop caused by X-ray irradiation. On the other hand, microwave treatment (16 Hz-modulated 2.45 GHz wave, 1 mW/cm2 power density, 1 h exposition, samples at 1 and 3 h after exposition) did not cause dearrangement but, rather an increase in the integration of thight junctional structure, which is in agreement with an increase in cytochemically detectable ZO-bound calcium.

Keywords

Tight junction zonula occludens freeze-fracture X-ray microwave 

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© Akadémiai Kiadó, Budapest 2001

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of General ZoologyEötvös Loránd UniversityBudapestHungary
  2. 2.“Fodor József” National Center of Public HealthNational “Frédéric Joliot-Curie” Research Institute for Radiobiology and RadiohygieneBudapestHungary

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