Cell aggregation (flocculation) of the yeast Schizosaccharomyces pombe strain RIVE 4-2-1 developed in glucose-containing medium, but only in the presence of ethanol. Cell surface proteins which participated in cell to cell interactions were characterised by the susceptibility of flocculation to different proteolytic enzymes, heat treatment, denaturing and thiol compounds and by the inhibition of flocculation by sugars and derivatives. It was shown that a galactose-specific lectin was involved in this new type of flocculation.
Al-Mahmood S., Giummelly S. P., Bonaly R., Delmotte F., Monsigny, M. (1988) Kluyveromyces bulgaricus yeast lectin. J. Biol. Chem. 263, 3930–3934.
Hodgson J. A., Berry D. R., Johnston, J. R. (1985) Discrimination by heat and protease treatment between flocculent phenotypes conferred on Saccharomyces cerevisiae by the genes FLOl and FL05. J. Gen. Microbiol. 131, 3219–3227.
Horisberger M., Rossett, J. (1977) Localization of a-galactomannan on the surface of Schizosaccharomycespom.be cells by scanning electron microscopy. Arch. Microbiol. 112, 123–126.
Hussain T., Salhi O., Lematre J., Charpentier, C., Bonaly, R. (1986) Comparative studies of floc-culation and deflocculation of Saccharomyces uvarum and Kluyveromyces bulgaricus. Appl. Microbiol. Biotechnol. 23, 269–273.
Johnson B. F., Walker T., Calleja G. B., Seligy, V. L. (1988) Sexual co-flocculation and asexual self-flocculation in budding and fission yeasts: experimental establishment of fundamental difference. Can. J. Microbiol. 34, 1105–1107.
Mbawala A., Al-Mahmood S., Loppinet V., Bonaly, R. (1990) Acetolysis and ’H NMR studies on mannans isolated from very flocculent cells of Pichia pastoris IFP 206. J. Gen. Microbiol. 136, 1279–1284.
Miki B. L. A., Poon N. H., James A. P., Seligy, V. L. (1982) Possible mechanism for flocculation interaction governed by gene FLOl in Saccharomyces cerevisiae. J. Bacteriol. 150, 878–889.
Mill, P. J. (1964) The nature of interactions between flocculent cells in the flocculation of Saccharomyces cerevisiae. J. Gen. Microbiol. 35, 63–68.
Nishihara H., Toraya T., Fukui, S. (1982) Flocculation of cell wall of brewer’s yeast and effect of metal ions, protein denaturants and enzyme treatments. Arch. Microbiol. 131, 112–115.
Soares E. V., Teixeira J. A., Mota, M. (1994) Effect of cultural and nutritional conditions on the control of flocculation expression in Saccharomyces cerevisiae. Can. J. Microbiol. 40, 851–857.
Stratford, M. (1989) Yeast flocculation: Calcium specificity. Yeast 5, 487–496.
Stratford, M. (1992) Yeast flocculation: a new perspective. Adv. Microb. Physiol. 33, 1–71.
Stratford, M. (1992) Yeast flocculation: Receptor definition by mnn mutants and concanavalin A. Yeast S, 635–645.
Stratford M., Pearson, B. M. (1992) Lectin-mediated flocculation of the yeast Saccharomycodes lud-wzgzz NCYC 734. Lett. Appl. Microbiol. 14, 214–216.
Suzzi G., Romano, R., Benevelli, M. (1992) The flocculation of wine yeasts: biochemical and morphological characteristics in Zygosaccharomyces — flocculation in Zygosaccharomyces. A. van Leeuwenhoek 61, 317–322.
Taylor N. W., Orton, W. L. (1973) Effect of the alkaline earth metal salts on flocculence in Saccharomyces cerevisiae. J. Inst. Brew. 79, 294–297.
Taylor N. W., Orton, W. L. (1975) Calcium in flocculence of Saccharomyces cerevisiae. J. Inst. Brew. 81, 53–57.
Teunissen, A. W. R. H., Steensma, H. Y. (1995) Review: The dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family. Yeast 11, 1001–1013.
Dedicated to Professor Lajos Ferenczy on the occasion of his 70th birthday.
About this article
Cite this article
Maráz, A., Geleta, A. Ethanol-Induced Cell Aggregation (Flocculation) and its Physiological Background in Schizosaccharomyces pombe Rive 4-2-1. BIOLOGIA FUTURA 52, 231–239 (2001). https://doi.org/10.1556/ABiol.52.2001.2-3.7
- Schizosaccharomyces pombe