Advertisement

Acta Biologica Hungarica

, Volume 52, Issue 2–3, pp 365–373 | Cite as

Variability of Isozyme and Rapd Markers Among Isolates of Mucor genevensis

  • Cs. VágvölgyiEmail author
  • Zs. Kasza
  • Ildikó Nyilasi
  • Klára Ács
  • T. Papp
Article

Abstract

Mucor genevensis is a dimorphic and homothallic fungal species (Zygomycetes). Ten M. genevensis strains, each strain of the recently described new homothallic species (M. meguroense and M. hachijyoensis) and strains of M. hiemalis and M. piriformis (as outgroups for numerical analysis) were investigated. Five different enzyme systems (CAT, GDH, G6D, MDH and SOD) and five 10-bp random primers were used in isoenzyme and random amplified polymorphic DNA analyses, respectively. The data from these studies were subjected to numerical analyses. Substantial intraspecific variability was detected in M. genevensis with both of the methods applied. Though both the M. meguroense strain and the M. hachi-jyoensis strain revealed characteristic differences, they grouped closer to the homothallic M. genevensis than to the heterothallic M. piriformis and M. hiemalis strains.

Keywords

Genetic variability homothallism isoenzyme analysis Mucor genevensis RAPD analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anné, J., Peberdy, J. F. (1981) Characterisation of interspecific hybrids between Penicillium chryso-genum and P. roqueforti by iso-enzyme analysis. Trans. Br. Mycol. Soc. 77, 401–408.CrossRefGoogle Scholar
  2. 2.
    Beauchamp, C., Fridovich, J. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287.CrossRefGoogle Scholar
  3. 3.
    Brewer, G. J., Sing, C. F. (1970) An introduction to isozyme techniques. Academic Press, New York.Google Scholar
  4. 4.
    Havens, P. L. (1976) Comparative zone electrophoresis and mating experiments in the taxonomy of Mucor hiemalis. Mycotaxon 4, 218–232.Google Scholar
  5. 5.
    Hesseltine, C. W. (1954) The section Genevensis of the genus Mucor. Mycologia 46, 358–366.CrossRefGoogle Scholar
  6. 6.
    Kanouse, B. B. (1924) The life history of a new homothallic Mucor. Pap. Mich. Acad. Sci. 3, 120–130.Google Scholar
  7. 7.
    Leach, J., Finkelstein, D. B., Rambosek, J. A. (1986) Rapid miniprep of DNA from filamentous fungi. Fungal Genet. Newslett. 33, 32–33.Google Scholar
  8. 8.
    Mulvey, M., Vrijenhoek, R. C. (1981) Genetic variation among laboratory strains of the planorbid snail Biomphalaria glabrata. Biochem. Genet. 19, 1169–1182.CrossRefGoogle Scholar
  9. 9.
    Naumov, N. A. (1935) Opredelitel Mukorovych (Mucorales). 2nd revised ed. Bot. Inst. Acad. Sci. USSR, Moscow and Leningrad. 140 pp. (In Russian).Google Scholar
  10. 10.
    Papp, T., Vágvölgyi, Cs., Kerényi, Z., Nagy, Á., Michailides, T. J. (1997) DNA amplification polymorphisms of Mucor piriformis. Ant. Leeuwenhoek 72, 167–173.CrossRefGoogle Scholar
  11. 11.
    Podani, J. (1993) SYN-TAX-pc, Computer programs for multivariate data analysis in ecology and systematics. Version 5.0. User’s Guide. Scientia Publishing, Budapest.Google Scholar
  12. 12.
    Schipper, M. A. A. (1973) A study on variability in Mucor hiemalis and related species. Stud. Mycol. 4, 1–40.Google Scholar
  13. 13.
    Schipper, M. A. A. (1978) On certain species of Mucor with a key to all accepted species. Stud. Mycol. 17, 1–52.Google Scholar
  14. 14.
    Schipper, M. A. A. (1986) Mating ability and the species concept of the Zygomycetes. In: Rayner, A. D. M., Brasier, C. M., Moore, D. (eds) Evolutionary Biology of Fungi. Cambridge Univ. Press, pp. 261–269.Google Scholar
  15. 15.
    Sneath, P. H. A., Sokal, R. R. (1973) Numerical taxonomy. W. H. Freeman and Co., San Francisco.Google Scholar
  16. 16.
    Vágvölgyi, Cs., Papp, T., Palágyi, Zs., Michailides, T. J. (1996) Isozyme variation among isolates of Mucor piriformis. Mycologia 88, 602–607.CrossRefGoogle Scholar
  17. 17.
    Vágvölgyi, Cs., Vastag, M., Acs, K., Papp, T. (1999) Rhizomucor tauricus: a questionable species of the genus. Mycol. Res. 103, 612–618.CrossRefGoogle Scholar
  18. 18.
    Vastag, M., Papp, T., Kasza, Zs., Vágvölgyi, Cs. (1998) Differentiation of Rhizomucor species by means of carbon source utilization and isoenzyme analysis. J. Clin. Microbiol. 36, 2153–2156.PubMedPubMedCentralGoogle Scholar
  19. 19.
    Watanabe, T. (1994) Two new species of homothallic Mucor in Japan. Mycologia 86, 691–695.CrossRefGoogle Scholar
  20. 20.
    Williams, J. G. K., Kubelik, A. R., Rafalski, J. A., Tingey, S. V (1991) Genetic analysis with RAPD markers. In: Bennett, J. W., Lasure, L. L. (eds) More genetic manipulations in fungi. Academic Press, San Diego. pp. 431–439.CrossRefGoogle Scholar
  21. 21.
    Woodbury, W., Spencer, A. K., Stanmann, M. A. (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal. Biochem. 44, 301–305.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2001

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Cs. Vágvölgyi
    • 1
    Email author
  • Zs. Kasza
    • 1
  • Ildikó Nyilasi
    • 2
  • Klára Ács
    • 1
  • T. Papp
    • 1
  1. 1.Department of Microbiology, Faculty of SciencesUniversity of SzegedHungary
  2. 2.Department of Microbiology, Faculty of Sciences, HAS Microbiological Research GroupUniversity of SzegedSzegedHungary

Personalised recommendations