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Cereal Research Communications

, Volume 47, Issue 2, pp 345–355 | Cite as

Characterization of Pakistani Wheat Germplasm for High and Low Molecular Weight Glutenin Subunits Using SDS-PAGE

  • A. KhalidEmail author
  • A. Hameed
Article

Abstract

High molecular weight (HMW-GS) and low molecular weight (LMW-GS) glutenin subunits play a significant role in bread making quality and extensibility, though they signify merely 10% and 40% of the entire seed storage proteins. For the estimation of bread quality on the basis of allelic difference in HMW-GS and LMW-GS at Glu-1 and 3 loci, wheat germplasm (77 genotypes) was collected from diverse agro-climatic regions of Pakistan and characterized by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Thirty distinct allelic arrangements were identified with a sum of thirteen Glu-1 alleles. Maximum frequency of allele 1 was found in twenty-nine genotypes at Glu-A1 locus while high proportion of subunit pairs 13 + 16 and 2 + 12 was detected in 33 and 32 genotypes at Glu-B1 as well as Glu-D1 locus, respectively. Few rare alleles were also separated out. The quality scores ranged from 4–10, however highest quality score of ten was more recurrent (36.36%). A good quality score of 8 and 6 were found in 32.47% as well as 19.48% of genotypes individually. In LMW-GS, seventeen diverse combinations of alleles with aggregate of ten Glu-3 alleles were detected. Glu-A3c and Glu-B3d alleles were observed in 33 (42.85%) genotypes, encoding high sedimentation and protein contents. Hence, this will enable the breeders to utilize both glutenin subunits as biochemical indicator for selecting superior wheat genotypes possessing enhanced bread making quality.

Keywords

wheat bread making quality HMW-GS LMW-GS SDS-PAGE 

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Copyright information

© Akadémiai Kiadó, Budapest 2019

Authors and Affiliations

  1. 1.Nuclear Institute for Agriculture and Biology (NIAB)FaisalabadPakistan
  2. 2.Pakistan Institute of Engineering and Applied SciencesIslamabadPakistan

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