Obtaining of Winter Rye (Secale Cereale L. ssp. Cereale) Haploid Embryos through Hybridization with Maize (Zea Mays L.)
The aim of this study was to determine the effect of selected factors on rye (Secale cereale L.) haploid embryo production by the wide crossing method. The study was performed on fifteen winter rye genotypes. This is the first time for rye when besides the genotype, on the enlargement of ovaries and haploid embryo production, such factors as: type of auxin analogues 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropyridine-2-carboxylic acid (picloram), and the time between florets emasculation and pollination were investigated. All factors had a significant impact on rye ovary enlargement, however the haploid embryo formation depended only on rye genotype, not on kind of auxin and days between emasculation to pollination. In total, twenty one haploid embryos were formed by six genotypes of fifteen tested. On average, 13.86% (after 2,4-D treatment) to 20.05% (after dicamba treatment) enlarged ovaries per emasculated florets were obtained. Most of the ovaries enlarged when florets were pollinated 4 and 6 days after emasculation. Most of the haploid embryos formed when florets were pollinated 6 days after emasculation. The obtained haploid embryos did not germinate.
Keywordsauxin haploid embryo maize rye wide crossing
Unable to display preview. Download preview PDF.
- Altenhofer, P., Oertel, C., Matzk, F. 1997. Chromosome elimination in wide crosses of Poaceae. Current topics in plant cytogenetics related to plant improvement. International Symposium held at Tulln, Austria, February 21–22, pp. 310–317.Google Scholar
- Deimling, S., Flehinghaus-Roux, T., Rober, F., Schechert, A., Roux, S.R., Geiger, H.H. 1994. Doubled haploid production-now reproducible in rye. In: Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, June 12–17, p. 95.Google Scholar
- Hoagland, D.R., Arnon, D.I. 1938. A water culture method for growing plants without soil. Circ. Univ. Calif., Agric. Exp. Stn., No. 347.Google Scholar
- Hörlein, A.J. 1991. Metodische Untersuchungen zur Antherenkultur bei Roggen. Metodische Untersuchungen zur Antherenkultur bei Roggen. Dissertation zur Erlagung des Grades eines Doktors der Agrarwissenschaften, Universität Hohenheim, Stuttgart.Google Scholar
- Hromada-Judycka, A., Bolibok-Brągoszewska, H., Rakoczy-Trojanowska, M. 2010. Genetically directed differential subtraction chain products related to in vitro response of immature embryos of rye (Secale cereale L.): isolation, characterization, and expression analysis Plant Cell Tiss. Org. Cult. 100:131–138.CrossRefGoogle Scholar
- Immonen, S., Anttila, H. 1996. Success in anther culture of tye. Proc. EUCARPIA Int. Symp. Rye Breeding & Genetics. Vortr. Pflanzenziichtg. 35:237–244.Google Scholar
- Mikołajczyk, S., Broda, Z., Weight, D. 2012. The effect of cold temperature stress on the viability of rye (Secale cereale L.) microspores. J. Biotech. 93(2):139–142.Google Scholar
- Noga A., Skrzypek, E., Warchoł, M., Czyczyło-Mysza, I., Dziurka, K., Marcińska, I., Juzoń, K., Warzecha, T., Sutkowska, A., Nita, Z., Werwińska, K. 2016. Conversion of oat (Avena sativa L.) haploid embryos into plants in relation to embryo developmental stage and regeneration media. In Vitro Cellular & Developmental Biology – Plant 52:590–597.CrossRefGoogle Scholar
- Ponitka, A., Ślusarkiewicz-Jarzina, A. 2004. Cleared-ovule technique used for rapid access to elary embryo development in Secale cereale x Zea mays crosses. Acta Biol. Cracov. Series Botanica 46:133–137.Google Scholar
- Wenzel, G., Thomas, E. 1974. Observations on the growth in culture of anthers of Secale cereale. Z. Pflanzenzüchtg. 72:89–94.Google Scholar
- Zhuang, J.J., Xu, J. 1983. Increasing differentiation frequencies in wheat pollen callus. In: Hu, H., Vega, M.R. (eds) Cell and Tissue Culture Techniques for Cereal Crop Improvement, Science Press, Beijing, p. 431.Google Scholar