Novel D-hordein-like HMW Glutenin Sequences Isolated from Psathyrostachys juncea by Thermal Asymmetric

Abstract

New high-molecular-weight glutenin (HMW glutenin) sequences isolated from six Psathyrostachys juncea accessions by thermal asymmetric interlaced PCR differ from previous sequences from this species. They showed novel modifications in all of the structural domains, with unique C-terminal residues, and their N-terminal lengths were the longest among the HMW glutenins reported to date. In their repetitive domains, there were three repeatable motif units: 13-residue [GYWH(/I/Y)YT(/Q)S(/T)VTSPQQ], hexapeptide (PGQGQQ), and tetrapeptide (ITVS). The 13-residue repeats were restricted to the current sequences, while the tetrapeptides were only shared by D-hordein and the current sequences. However, these sequences were not expressed as normal HMW glutenin proteins because an in-frame stop codon located in the C-termini interrupted the intact open reading frames. A phylogenetic analysis supported different origins of the P. juncea HMW glutenin sequences than that revealed by a previous study. The current sequences showed a close relationship with D-hordein but appeared to be more primitive.

References

  1. Anderson, O.D., Greene, F.C. 1989. The characterization and comparative analysis of high molecular weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theor. Appl. Genet. 77:689–700.

    CAS  Article  Google Scholar 

  2. Cao, Z., Deng, Z., Wang, M., Wang, X., Jing, J., Zhang, X., Shang, H., Li, Z. 2008. Inheritance and molecular mapping of an alien stripe-rust resistance gene from a wheat-Psathyrostachys huashanica translocation line. Plant Sci. 174:544–549.

    CAS  Article  Google Scholar 

  3. Garg, M., Tanaka, H., Tsujimoto, H. 2009. Exploration of Triticeae seed storage proteins for improvement of wheat end product quality. Breed. Sci. 59:519–528.

    CAS  Article  Google Scholar 

  4. Gu, Y.Q., Anderson, O.D., Londeorë, C.F., Kong, X.Y., Chibbar, R.N., Lazo, G.R. 2003. Structural organization of the barley D-hordein locus in comparison with its orthologous regions of wheat genomes. Genome 46:1084–1097.

    CAS  Article  Google Scholar 

  5. Hsiao, C., Wang, R.R-C., Dewey, D.R. 1986. Karyotype analyses and genome relationships of 22 diploid species in the tribe Triticeae. Can. J. Genet. Cytol. 28:109–120.

    Article  Google Scholar 

  6. Kong, L.N., Liang, Y., Qin, L.M., Sun, L., Xia, G.M., Liu, S.W. 2014. Characterization of high molecular weight glutenin subunit genes from the Ns genome of Psathyrostachys juncea. Dev. Genes Evol. 224:189–196.

    CAS  Article  Google Scholar 

  7. Liu, Y.G., Cheng, Y.L. 2007. High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. Biotechniques 43:649–656.

    CAS  Article  Google Scholar 

  8. Mackie, A.M., Lagudah, E.S., Sharp, P.J., Lafiandra, D. 1996. Molecular and biochemical characterisation of HMW glutenin subunits from T. tauschii and the D genome of hexaploid wheat. J. Cereal Sci. 23:213–225.

    CAS  Article  Google Scholar 

  9. Mujeeb-Kazi, A., Cortes, A., Riera-Lizarazu, O. 1995. The cytogenetics of a Triticum turgidum × Psathyrostachys juncea hybrid and its backcross derivatives. Theor. Appl. Genet. 90:430–437.

    CAS  Article  Google Scholar 

  10. Pistón, F., Shewry, P.R., Barro, F. 2007. D hordeins of Hordeum chilense: a novel source of variation for improvement of wheat. Theor. Appl. Genet. 115:77–86.

    Article  Google Scholar 

  11. Plourde, A., Fedak, G., St-Pierre, C.A., Comeau, A. 1990. A novel intergeneric hybrid in the Triticeae: Triticum aestivum × Psathyrostachys juncea. Theor. Appl. Genet. 79:45–48.

    CAS  Article  Google Scholar 

  12. Rasheed, A., Xia, X.C., Yan, Y.M., Appels, R., Mahmood, T., He, Z.H. 2014. Wheat seed storage proteins: Advances in molecular genetics, diversity and breeding applications. J. Cereal Sci. 60:11–24.

    CAS  Article  Google Scholar 

  13. Shewry, P.R., Halford, N.G., Tatham, A.S., Popineau, Y., Lafiandra, D., Belton, P.S. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing properties. Adv. Food Nutr. Res. 45:219–302.

    CAS  Article  Google Scholar 

  14. Sugiyama, T., Rafalski, A., Peterson, D., Soll, D. 1985. A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res. 13:8729–8737.

    CAS  Article  Google Scholar 

  15. Sun, Y.Q., Pu, Z.J., Dai, S.F., Pu, X.X., Liu, D.C., Wu, B.H., Lan, X.J., Wei, Y.M., Zheng, Y.L., Yan, Z.H. 2014. Characterization of y-type high-molecular-weight glutenins in tetraploid species of Leymus. Dev. Genes Evol. 224:57–64.

    CAS  Article  Google Scholar 

  16. Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725–2729.

    CAS  Article  Google Scholar 

  17. Wan, Y.F., Yan, Z.H., Liu, K.F., Zheng, Y.L., D’ Ovidio, R., Shewry, P.R., Halford, N.G., Wang, D.W. 2005. Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin. Theor. Appl. Genet. 111:1183–1190.

    CAS  Article  Google Scholar 

  18. Wang, X.Q., Yan, Z.H., Dai, S.F., Liu, D.C., Wei, Y.M., Zheng, Y.L. 2012. Molecular characterization of four HMW glutenin genes from Heteranthelium piliferum C. E. Hubbard and Henrardia persica (Banks et Solander) Hochstetter. Genet. Resour. Crop Evol. 59:1309–1318.

    CAS  Article  Google Scholar 

  19. Cereal Research Communications 46, 2018

  20. Yang, F., Yan, Z.H., Dai, S.F., Han, C., Hu, X.G. 2010. Isolation and characterization of upstream sequences of two high molecular weight glutenin subunit genes from Psathyrostachys juncea. J. Sichuan Agricultural University 28:397–402.

    Google Scholar 

  21. Yuan, Z.W., Chen, Q.J., Zhang, L.Q., Yan, Z.H., Zheng, Y.L., Liu, D.C. 2009. Molecular characterization of two silenced y-type genes for Glu-B1 in Triticum aestivum ssp. yunnanese and ssp. tibetanum. J. Integr. Plant Biol. 51:93–99.

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The authors acknowledge financial support from the National Natural Science Foundation of China (U1403185) and the Key Fund Project of the Sichuan Provincial Department of Education (15ZA0021).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Z. H. Yan.

Additional information

Communicated by F. Békés

Electronic Supplementary Material (ESM)

Rights and permissions

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Dai, S.F., Xue, X.F., Wang, Y.F. et al. Novel D-hordein-like HMW Glutenin Sequences Isolated from Psathyrostachys juncea by Thermal Asymmetric. CEREAL RESEARCH COMMUNICATIONS 46, 135–145 (2018). https://doi.org/10.1556/0806.45.2017.060

Download citation

Keywords

  • Psathyrostachys juncea
  • HMW glutenin
  • sequence analysis
  • thermal asymmetric interlaced PCR
  • phylogenetic analysis