Effect of Seed Pre-sowing Gamma-irradiation Treatment in Bread Wheat Lines Differing by Anthocyanin Pigmentation
Anthocyanins are natural antioxidants able to scavenge free radicals, which appear in plant cells under various environmental stresses. In wheat, anthocyanin pigments can be synthesized in vegetative and reproductive organs. The objective of the current study was to estimate the significance of these substances for wheat seedlings protection under irradiation stress (after treatment of dry seeds with moderate doses of gamma-irradiation, 50, 100 and 200 Gy). For this goal a set of near-isogenic lines (8 NILs) carrying different combinations of the Pp (purple pericarp) and Rc (red coleoptile) alleles were used. The effect of gammairradiation on the growth parameters and anthocyanin content in coleoptiles was studied at the 4th day after germination. The germination rate was not affected, while roots’ and shoots’ lengths and fresh weights as well as root number decreased significantly under irradiation treatment. The effect was deeper under higher doses. Irradiation treatment also induced change of root morphology (‘hairy roots’). The effect of treatment on coleoptile anthocyanin content depended on allelic combination at the Rc loci. At the presence of ‘weak’ Rc-A1 allele anthocyanin content decreased, while it did not change in lines with Rc-A1 + Rc-D1 combination (NILs with intensively colored coleoptiles). Factors ‘pericarp color’ and ‘coleoptile color’ influenced vigor of the seedlings under 50 Gy, whereas under higher doses (100 and 200 Gy) these factors did not contribute to growth parameters changes. Statistically significant positive effect of anthocyanins synthesized in coleoptile (in the presence of Rc-A1 + Rc-D1 dominant alleles) on root growth of seedling germinated from 50 Gy-treated seeds was observed.
Keywordsanthocyanins coleoptile color gamma-irradiation pericarp color plant vigor protective effect Pp genes Rc genes seed germination seedlings growth Triticum aestivum L.
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This work was supported by the Russian Science Foundation (project 16-14-00086). Growing of wheat plants in ICG Plant Growth Core Facility was supported by the ICG project 0324-2016-001. We thank Ms Galina Generalova and Mrs Olga Zakharova for technical assistance and Dr Elena Antonova for fruitful discussion.
- Abou-Zeid, H.M., Abdel-Latif, S.A. 2014. Effects of gamma irradiation on biochemical and antioxidant defense system in wheat (Triticum aestivum L.) seedlings. Int. J. Adv. Res. 2:287–300.Google Scholar
- Arbuzova, V.S., Maystrenko, O.I., Popova, O.M. 1998. Development of near-isogenic lines of the common wheat cultivar ‘Saratovskaya 29’. Cereal Res. Commun. 26:39–46.Google Scholar
- Caverzan, A., Casassola, A., Patussi Brammer, S.P. 2016. Reactive oxygen species and antioxidant enzymes involved in plant tolerance to stress. In: Shanker, A. (ed.), Abiotic and Biotic Stress in Plants – recent advances and future perspectives. InTech. Rijeka, Croatia. pp. 463–480.Google Scholar
- Hong, M.J., Kim, J.B., Yoon, Y.H., Kim, S.H., Ahn, J.W., Jeong, I.Y., Kang, S.Y., Seo, Y.W., Kim, D.S. 2014. The effects of chronic gamma irradiation on oxidative stress response and the expression of anthocyanin biosynthesis-related genes in wheat (Triticum aestivum). Int. J. Radiat. Biol. 17:1–31.Google Scholar
- Khlestkina, E.K. 2012. Genes determining coloration of different organs in wheat. Vavilovskii Zhurnal Genetiki i Selektsii (Vavilov J. of Genetics and Breeding) 16:202–216.Google Scholar
- Karabanov, I.A., Veremeitschik, V.Ye. 1972. The influence of irradiation on the productivity and the content of polyphenols in buckwheat. Dokl. AN SSSR. 203:488–490. (in Russian)Google Scholar
- Mohajer, S., Taha, R.M., Lay, M.M., Esmaeili, A.K., Khalili, M. 2014. Stimulatory effects of gamma irradiation on phytochemical properties, mitotic behaviour, and nutritional composition of sainfoin (Onobrychis viciifolia Scop.). Sci. World. J. Article ID 854093:1–9. http://dx.doi.org10.1155/2014/854093.
- Ramabulana, T., Mavunda, R.D., Steenkamp, P.A., Piater, L.A., Dubery, I.A., Madala, N.E. 2016. Perturbation of pharmacologically relevant polyphenolic compounds in Moringa oleifera against photo-oxidative damages imposed by gamma radiation. J. Photochem. Photobiol. B. 156:79–86.CrossRefGoogle Scholar