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Cereal Research Communications

, Volume 46, Issue 1, pp 21–30 | Cite as

Hydrogen Peroxide is Involved in the Regulation of Ascorbate and Glutathione Metabolism in Wheat Leaves under Water Stress

  • C. ShanEmail author
  • X. Ou
Physiology

Abstract

This study investigated the regulation of ascorbic acid and glutathione metabolism by hydrogen peroxide in wheat leaves under water stress. The results showed that H2O2 level, the activities of ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase, L-galactono-1,4-lactone dehydrogenase and gamma-glutamylcysteine synthetase, and the contents of reduced ascorbate, reduced glutathione, total ascorbate and total glutathione were increased by water stress. And above increases were suppressed by pre-treatment with NADPH oxidase inhibitor diphenyleneiodonium chloride. Application of DPI also reduced H2O2 level, the activities of γ-ECS, GalLDH, APX, GR, DHAR and MDHAR, and the contents of AsA, GSH, total ascorbate and total glutathione, compared with control. Application of H2O2 to DPI-inhibited wheat seedlings prevented the reduction in the activities of APX, GR, MDHAR, DHAR, GalLDH and γ-ECS, and the contents of AsA, GSH, total ascorbate and total glutathione induced by DPI under water stress. Meanwhile, pre-treatment with DPI increased the malondialdehyde content and electrolyte leakage. Application of H2O2 to DPI-inhibited wheat seedlings prevented the increases in MDA content and EL. Our results suggested that water stress-induced H2O2 is a signal that leads to the up-regulation of ascorbate and glutathione metabolism and has an important role for acquisition of water-stress tolerance in wheat.

Keywords

water stress hydrogen peroxide ascorbate glutathione wheat 

Abbreviations

APX

ascorbate peroxidase

DHAR

dehydroascorbate reductase

DPI

diphenyleneiodonium chloride

EL

electrolyte leakage

γ-ECS

gamma-glutamylcysteine synthetase

GR

glutathione reductase

H2O2

hydrogen peroxide

GalLDH

L-galactono-1,4-lactone dehydrogenase

MDA

malondialdehyde

MDHAR

monodehydroascorbate reductase

AsA

reduced ascorbate

GSH

reduced glutathione

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Notes

Acknowledgements

Our study was funded by “Open project of Crop Science Characteristic Discipline of Henan Province” and “Important Science and Technology Specific Project of Henan Province (151100110700)”.

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Copyright information

© Akadémiai Kiadó, Budapest 2018

Authors and Affiliations

  1. 1.School of Science and TechnologyHenan Institute of Science and TechnologyXinxiangChina
  2. 2.Collaborative Innovation Center of Modern Biological BreedingHenan Province, XinxiangChina

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