Cereal Research Communications

, Volume 45, Issue 4, pp 621–632 | Cite as

Enhancement of Leaf Rust Resistance of Siberian Winter Wheat Varieties by Marker-assisted Selection

  • I. N. LeonovaEmail author
  • A. I. Stasyuk
  • E. S. Skolotneva
  • E. A. Salina


Cultivation of winter wheat varieties in the West Siberian region of Russia has competitive advantages compared to spring varieties: utilization of spring-summer moisture, early maturation and harvest and a high yield potential. The poor resistance of winter varieties to foliar diseases results in significant yield losses and facilitates the spread of pathogens to the spring wheat cultivars. The present study was conducted to evaluate the effectiveness of molecular markers specific for VRN-1 and Lr loci in selecting winter wheat genotypes resistant to leaf rust. The winter wheat cultivars Biyskaya ozymaya and Filatovka were crossed with spring wheat introgression lines 21-4 and 5366-180 and the spring wheat cultivar Tulaikovskaya 10 carrying LrTt2, LrAsp5 and Lr6Ai#2 loci from Triticum timopheevii, Aegilops speltoides and Thynopyrum intermedium, respectively. To identify winter wheat plants homozygous for target loci, F2 populations were screened with functional markers to VRN-1 genes and with markers specific for alien genetic material. Based on the genotyping analysis of 371 F2 plants a total of 44 homozygous genotypes with winter habit was identified. There were eight genotypes containing Lr loci among them. Evaluation of F2-derived F3–4 families for both seedling and adult resistance showed that only one F3–4 family had moderate susceptible reaction type to the field population of leaf rust. Others ranged from nearly immune to resistant with severity of 5%. The data also indicated the utility of the VRN-1 allele-specific markers for detection of genotypes with winter habit without vernalization at early stages of plant breeding.


winter and spring wheats leaf rust VRN-1 genes Lr genes marker-assisted selection 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.



We thank the Russian Science Foundation (project no. 16-16-00011) for financial support of this study. We thank the Joint Access Center for Artificial Plant Cultivation supported by the IC&G Budgetary Project No. 0324-2015-0005. I.N. Leonova and A.I. Stasyuk have contributed equally to this work.


  1. Baenziger, P.S., DePauw, R.M. 2009. Wheat breeding: procedures and strategies. In: Carver, B.F. (ed.), Wheat Science and Trade. Wiley-Blackwell. Oxford, UK. pp. 275–308.Google Scholar
  2. Cockram, J., Norris, C., Donal, M., O’Sullivan, D.M.O. 2009. PCR-based markers diagnostic for spring and winter seasonal growth habit in barley. Crop Sci. 49:403–410.CrossRefGoogle Scholar
  3. Deng, W., Casao, M.C., Wang, P., Sato, K., Hayes, P.H., Finnegan, E.J., Trevaskis, B. 2015. Direct links between the vernalization response and other key traits of cereal crops. Nature Commun. 6:5882.CrossRefGoogle Scholar
  4. Fu, D., Szücs, P., Yan, L., Helguera, M., Skinner, J.S., Zitzewitz, J.V., Hayes, P.M., Dubcovsky, J. 2005. Large deletions within the frst intron in VRN-1 are associated with spring growth habit in barley and wheat. Mol. Genet. Genomics 273:54–65.CrossRefGoogle Scholar
  5. Gultyaeva, E.I., Dmitriev, A.P., Kosman, E. 2012. Regional diversity of Russian populations of Puccinia triticina in 2007. Can. J. Plant Pathol. 34:213–224.CrossRefGoogle Scholar
  6. Gultyaeva, E.I., Shaidayuk, E.L., Kazartsev, I.A., Aristova, M.K. 2015. Structure of Russian populations of Puccinia triticina Eriks. Plant Protection News 3:5–10.Google Scholar
  7. Ishikawa, G., Nakamura, T., Ashida, T., Saito, M., Nasuda, S., Endo, T.R., Wu, J., Matsumoto, T. 2009. Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers. Theor. Appl. Genet. 118:499–514.CrossRefGoogle Scholar
  8. Kant, L., Mani, V.P., Gupta, H.S. 2008. Winter × spring wheat hybridization – A promising avenue for yield enhancement. Plant Breed. 120:255–258.CrossRefGoogle Scholar
  9. Kiseleva, A.A., Shcherban, A.B., Leonova, I.N., Frenkel, Z., Salina, E.A. 2016. Identification of new heading date determinants in wheat 5B chromosome. BMC Plant Biol. 16 (Suppl. 1):8.CrossRefGoogle Scholar
  10. Kolmer, J.A., Kabdulova, M.G., Mustafina, M.A., Zhemchuzhina, N.S., Dubovoy, V. 2015. Russian populations of Puccinia triticina in distant regions are not differentiated for virulence and molecular genotype. Plant Pathol. 64:328–336.CrossRefGoogle Scholar
  11. Lapochkina, I.F., Baranova, O.A., Shamanin, V.P., Volkova, G.V., Gainullin, N.R., Anisimova, A.V., Galinger, D.N., Lazareva, E.N., Gladkova, E.V., Vaganova, O.F. 2016. The development of initial material of spring common wheat for breeding for resistance to stem rust (Puccinia graminis Pers. f. sp. tritici), including race Ug99, in Russia. Vavilov J. Genet. Breeding 20:320–328.CrossRefGoogle Scholar
  12. Mains, E.B., Jackson, H.S. 1926. Physiological specialization in the leaf rust of wheat, Puccinia triticina Erikss. Phytopathol. 16:89–120.Google Scholar
  13. Mallick, N., Vinod, S.J.B., Sharma, J.B., Tomar, R.S., Sivasamy, M., Prabhu, K.V. 2015. Marker-assisted back-cross breeding to combine multiple rust resistance in wheat. Plant Breed. 134:172–177.CrossRefGoogle Scholar
  14. McIntosh, R.A., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Appels, R., Xia, X.C. 2013. Catalogue of Gene Symbols for Wheat.
  15. Miedaner, T., Korzun, V. 2012. Marker-assisted selection for disease resistance in wheat and barley breeding. Phytopathol. 102:560–566.CrossRefGoogle Scholar
  16. Morgounov, A.I., Vlasenko, V.A., McNab, A., Braun, H.J. 1996. Wheat breeding: Objectives, methodology, and progress. Scholar
  17. Morgounov, A., Ablova, L., Babayants, O., Babayants, L., Bespalova, L., Khudokormov, Zh., Litvinenko, N., Shamanin, V., Syukov, V. 2011. Genetic protection of wheat rusts and development of resistant varieties in Russia and Ukraine. Euphytica 179:297–311.CrossRefGoogle Scholar
  18. Nowak, M., Leśniowska-Nowak, J., Zapalska, M., Banaszak, Z., Kondracka, K., Dudziak, K., Kowalczyk, K. 2014. Analysis of VRN1 gene in triticale and common wheat genetic background. Scientia Agricola 71:345–355.CrossRefGoogle Scholar
  19. Olson, E., Brown-Guedira, G., Marshall, D., Stack, E., Bowden, R.L., Jin, Y., Rouse, M., Pumphrey, M.O. 2010. Development of lines with a small introgressed segment carrying stem rust resistance gene Sr22. Crop Sci. 50:1823–1830.CrossRefGoogle Scholar
  20. Peterson, R.F., Campbell, A.B., Hannah, A.E. 1948. A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can. J. Research Sect. C 26:496–500.CrossRefGoogle Scholar
  21. Petrash, N.V., Leonova, I.N., Adonina, I.G., Salina, E.A. 2016. Effect of translocations from Aegilops speltoides Tausch on resistance to fungal diseases and productivity in common wheat. Rus. J. Genet. 52:1253–1262.CrossRefGoogle Scholar
  22. Pugsley, A.T. 1971. A genetic analysis of spring-winter habit in wheat. Austr. J. Agric. Res. 22:21–31.CrossRefGoogle Scholar
  23. Rizza, F., Karsai, L., Morcia, K., Badeck, F.W., Terzi, V., Pagani, D., Kiss, T., Stanca, A.M. 2016. Association between the allele compositions of major plant developmental genes and frost tolerance in barley (Hordeum vulgare L.) germplasm of different origin. Mol Breed. 36:156–172.CrossRefGoogle Scholar
  24. Roelfs, A.P., Singh, R.P., Saari, E.E. 1992. Rust diseases of wheat: concepts and methods of disease management. CIMMYT. Mexico.Google Scholar
  25. Salina, E.A, Adonina, I.G., Badaeva, E.D., Kroupin, P.Yu., Stasyuk, A.I., Leonova, I.N., Shishkina, A.A., Divashuk, M.G., Starikova, E.V., Khuat Thi Mai, L., Syukov, V.V., Karlov, G.I. 2015. A Thinopyrum intermedium chromosome in bread wheat cultivars as a source of genes conferring resistance to fungal diseases. Euphytica 204:91–101.CrossRefGoogle Scholar
  26. Sanin, S.S., Nazarova, A.N. 2010. The phytosanitary situation in the wheat felds in the Russian Federation (1991–2008). Plant Protection and Quarantine 2:70–78. (in Russian)Google Scholar
  27. Sharma, S., Chaudhary, H.R. 2009. Combining ability and gene action studies for yield-contributing traits in crosses involving winter and spring wheat genotypes. Acta Agronomica Hungarica 57:417–423.CrossRefGoogle Scholar
  28. Shcherban, A.B., Efremova, T.T., Salina, E.A. 2012. Identification of a new Vrn-B1 allele using two near-isogenic wheat lines with difference in heading time. Mol. Breed. 29:675–685.CrossRefGoogle Scholar
  29. Shcherban, A.B., Börner, A., Salina, E.A. 2015. Effect of VRN-1 and PPD-1 genes on heading time of European bread wheat cultivars. Plant Breed. 134:49–55.CrossRefGoogle Scholar
  30. Somers, D.J., Isaac, P., Edwards, K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 109:1105–1114.CrossRefGoogle Scholar
  31. Song, Q.J., Shi, J.R., Singh, S., Fickus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.B. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110:550–560.CrossRefGoogle Scholar
  32. Sutka, J. 2001. Genes for frost resistance in wheat. Euphytica 119:167–172.CrossRefGoogle Scholar
  33. Timonova, E.M., Leonova, I.N., Röder, M.S., Salina, E.A. 2013. Marker-assisted development and characterization of a set of Triticum aestivum lines carrying different introgressions from the T. timopheevii genome. Mol. Breed. 31:123–136.CrossRefGoogle Scholar
  34. Witcombe, J., Virk, D. 2001. Number of crosses and population size for participatory and classical plant breeding. Euphytica 122:451–462.CrossRefGoogle Scholar
  35. Xu, Y. 2010. Molecular Plant Breeding. CAB International. Cambridge, MA, USA.CrossRefGoogle Scholar
  36. Yan, L., Helguera, M., Kato, K., Fukuyama, S., Sherman, J., Dubcovsky, J. 2004. Allelic variation at the VRN-1 promoter region in polyploid wheat. Theor. Appl. Genet. 109:1677–1686.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • I. N. Leonova
    • 1
    Email author
  • A. I. Stasyuk
    • 1
  • E. S. Skolotneva
    • 1
  • E. A. Salina
    • 1
  1. 1.Federal Research Center Institute of Cytology and Genetics SB RASNovosibirskRussia

Personalised recommendations