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Cereal Research Communications

, Volume 45, Issue 4, pp 633–646 | Cite as

Identifying some Additional Rust Resistance Genes in Indian Wheat Varieties Using Robust Markers

  • H. Khan
  • S. C. BhardwajEmail author
  • O. P. Gangwar
  • P. Prasad
  • P. L. Kashyap
  • S. Savadi
  • S. Kumar
  • R. Rathore
Physiology
First Online:

Abstract

A set of forty wheat cultivars comprising bread wheat, durum and triticale identified during 2010–2014 were tested for resistance to Indian pathotypes of leaf, stem and yellow rusts at seedling stage under controlled conditions. Eight Lr genes (Lr1, Lr3, Lr10, Lr13, Lr14a, Lr23, Lr24 and Lr26) were characterized based on differential interactions with specific rust races. Genes Lr23, Lr26 and Lr13 conferred leaf rust resistance in most of the accessions. Three Yr genes (YrA, Yr2 and Yr9) were inferred in 40 genotypes, where Yr2 followed by Yr9 were most frequent in conferring stripe rust resistance. Ten Sr genes, namely, Sr2, Sr5, Sr8a, Sr7b, Sr9b, Sr9e, Sr11, Sr13, Sr24 and Sr31, were postulated in these lines with predominance of Sr11, Sr31 and Sr2. These Lr, Sr and Yr genes were observed singly or in combination. Robust DNA markers were used to identify adult plant resistance genes Yr18/Lr34/Sr57, Lr68 and Sr2 and all stage resistance genes Lr24/Sr24, Sr28 and Yr9/Lr26/Sr31. STS marker iag95 showed presence of Yr9 in four additional cultivars which were resistant to one or more rusts. Gene Sr28 was identified in seven durum cultivars with the wPt7004 marker. This is first report of Sr28 being present in many Indian wheat cultivars. CsGs-STS marker identified Lr68 in nine cultivars.

Keywords

wheat Puccinia rust resistance gene postulation molecular marker 
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Notes

Acknowledgement

We are grateful to the Director, ICAR-IIWBR, Karnal, Haryana, 132 001-India for providing the necessary facilities and funds to carry out the research.

Supplementary material

42976_2017_4504633_MOESM1_ESM.pdf (152 kb)
Identifying some Additional Rust Resistance Genes in Indian Wheat Varieties Using Robust Markers

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© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • H. Khan
    • 1
  • S. C. Bhardwaj
    • 1
    Email author
  • O. P. Gangwar
    • 1
  • P. Prasad
    • 1
  • P. L. Kashyap
    • 1
  • S. Savadi
    • 1
  • S. Kumar
    • 1
  • R. Rathore
    • 1
  1. 1.ICAR - Indian Institute of Wheat and Barley ResearchFlowerdale, ShimlaIndia

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