Advertisement

Cereal Research Communications

, Volume 45, Issue 4, pp 633–646 | Cite as

Identifying some Additional Rust Resistance Genes in Indian Wheat Varieties Using Robust Markers

  • H. Khan
  • S. C. BhardwajEmail author
  • O. P. Gangwar
  • P. Prasad
  • P. L. Kashyap
  • S. Savadi
  • S. Kumar
  • R. Rathore
Physiology

Abstract

A set of forty wheat cultivars comprising bread wheat, durum and triticale identified during 2010–2014 were tested for resistance to Indian pathotypes of leaf, stem and yellow rusts at seedling stage under controlled conditions. Eight Lr genes (Lr1, Lr3, Lr10, Lr13, Lr14a, Lr23, Lr24 and Lr26) were characterized based on differential interactions with specific rust races. Genes Lr23, Lr26 and Lr13 conferred leaf rust resistance in most of the accessions. Three Yr genes (YrA, Yr2 and Yr9) were inferred in 40 genotypes, where Yr2 followed by Yr9 were most frequent in conferring stripe rust resistance. Ten Sr genes, namely, Sr2, Sr5, Sr8a, Sr7b, Sr9b, Sr9e, Sr11, Sr13, Sr24 and Sr31, were postulated in these lines with predominance of Sr11, Sr31 and Sr2. These Lr, Sr and Yr genes were observed singly or in combination. Robust DNA markers were used to identify adult plant resistance genes Yr18/Lr34/Sr57, Lr68 and Sr2 and all stage resistance genes Lr24/Sr24, Sr28 and Yr9/Lr26/Sr31. STS marker iag95 showed presence of Yr9 in four additional cultivars which were resistant to one or more rusts. Gene Sr28 was identified in seven durum cultivars with the wPt7004 marker. This is first report of Sr28 being present in many Indian wheat cultivars. CsGs-STS marker identified Lr68 in nine cultivars.

Keywords

wheat Puccinia rust resistance gene postulation molecular marker 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgement

We are grateful to the Director, ICAR-IIWBR, Karnal, Haryana, 132 001-India for providing the necessary facilities and funds to carry out the research.

Supplementary material

42976_2017_4504633_MOESM1_ESM.pdf (152 kb)
Identifying some Additional Rust Resistance Genes in Indian Wheat Varieties Using Robust Markers

References

  1. Bansal, U.K., Hayden, M.J., Gill, M.B., Bariana, H.S. 2010. Molecular mapping of a seedling stripe rust resistance gene in wheat cultivar Rubric. Euphytica 171:121–127.CrossRefGoogle Scholar
  2. Bansal, U., Bariana, H., Wong, D., Randhawa, M., Wicker, T., Hayden, M., Keller, B. 2014. Molecular mapping of an adult plant stem rust resistance gene Sr56 in winter wheat cultivar Arina. Theor. Appl. Genet. 127:1441–1448.CrossRefGoogle Scholar
  3. Bariana, H.S., McIntosh, R.A. 1995. Genetics of adult plant stripe rust resistance in four Australian wheats and the French cultivar ‘Hybride de Bersee’. Plant Breed. 114:485–491.CrossRefGoogle Scholar
  4. Bariana, H.S., McIntosh, R.A. 1993. Cytogenetic studies in wheat XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482.PubMedGoogle Scholar
  5. Bariana, H.S., Brown, G.N., Ahmed, N.U., Khatkar S., Conner, R.L., Wellings, C.R., Haley, S., Sharp, P.J., Laroche, A. 2002. Characterization of Triticum vavilovii-derived stripe rust resistance using genetic, cytogenetic and molecular analyses and its marker-assisted selection. Theor. Appl. Genet. 104:315–320.CrossRefGoogle Scholar
  6. Bariana, H.S., Brown, G.N., Bansal, U.K., Miah, H., Standen, G.E., Lu, M. 2007. Breeding triple rust resistant wheat cultivars for Australia using conventional and marker-assisted selection technologies. Austr. J. Agric. Res. 58:576–587.CrossRefGoogle Scholar
  7. Bhardwaj, S.C. 2011. Resistance genes and adult plant resistance of released wheat varieties of India. Regional Station, Directorate of Wheat Research. Flowerdale, Shimla-171002 HP. India. Res. Bull. No. 5. 31 p.Google Scholar
  8. Bhardwaj, S.C., Nayar, S.K., Prashar, M., Jain, S.K., Singh, S.B. 2003. Diversity of resistance for Puccinia graminis tritici in wheat (Triticum aestivum) and triticale material. Indian J. Agric. Sci. 73:676–679.Google Scholar
  9. Bhardwaj, S.C. 2013. PucciniaTriticum interaction: an update. Indian Phytopathol. 66:14–19.Google Scholar
  10. Bhardwaj, S.C., Prashar, M., Jain, S. K., Kumar, S., Datta, D. 2010a. Adult plant resistance in some Indian wheat genotypes and postulation of leaf rust resistance genes. Indian Phytopathol. 63:174–180.Google Scholar
  11. Bhardwaj, S.C., Prashar, M., Jain, S.K., Kumar, S., Sharma, Y.P., Sivasamy, M. 2010b. Virulence of Puccinia triticina on Lr28 in wheat and its evolutionary relation to prevalent pathotypes in India. Cereal Res. Commun. 38:83–89.CrossRefGoogle Scholar
  12. Bhardwaj, S.C., Gangwar, O.P., Singh, S.B., Saharan, M.S., Sharma, S. 2012. Rust situation and pathotypes of Puccinia species in Leh Ladakh in relation to recurrence of wheat rusts in India. Indian Phytopathol. 65:230–232.Google Scholar
  13. Biffen, R.H. 1931. The cereal rusts and their control. Transactions of the British Mycological Society 16:19–37.CrossRefGoogle Scholar
  14. Browder, L.E. 1973. Probable genotype of some Triticum aestivum ‘Agent’ derivatives for reaction to Puccinia recondita f. sp. tritici. Crop Sci. 13:203–206.CrossRefGoogle Scholar
  15. Brown, G.N. 1997. The inheritance and expression of leaf chlorosis associated with gene Sr2 for adult plant resistance to wheat stem rust. Euphytica 95:67–71.CrossRefGoogle Scholar
  16. Don, R.H., Cox, P.T., Wainwright, B.J., Baker, K., Mattick, J.S. 1991. Touchdown PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.CrossRefGoogle Scholar
  17. Herrera-Foessel, S.A., Singh, R.P., Huerta-Espino, J., Rosewarne, G.M., Periyannan, S.K., Viccar, L., Calvo-Salazar, V., Lan, C., Lagudah, E.S. 2012. Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor. Appl. Genet. 124:1475–1486.CrossRefGoogle Scholar
  18. Johnson, R. 1988. Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding. In: Simmonds, N.W., Rajaram, S. (eds), Breeding Strategies for Resistance to the Rusts of Wheat. CIMMYT. Mexico. pp. 63–75.Google Scholar
  19. Kolmer, J.A. 1996. Genetics of resistance to wheat leaf rust. Annu. Rev. of Pathol. 34:435–455.Google Scholar
  20. Lagudah, E.S., Mcfadden, H., Singh, R.P., Heurta-Espino, J., Bariana, H.S., Spielmyer, W. 2006. Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theor. Appl. Genet. 114:21–30.CrossRefGoogle Scholar
  21. Loegering, W.Q., McIntosh, R.A., Burton, C.H. 1971. Computer analysis of disease data to drive hypothetical genotypes for reaction of host varieties to pathogens. Can. J. of Genet. and Cytol. 13:149–154.CrossRefGoogle Scholar
  22. Luig, N.H. 1983. A survey of virulence genes in wheat stem rust Puccinia graminis f. sp. tritici. Advances in Plant Breeding (Suppl. 11 to Plant Breeding). Verlag Paul Parey. Berlin, Germany.Google Scholar
  23. Mago, R., Bariana, H.S., Dundas, L.S., Speilmeyer, W., Lawrence, G.J., Pryor, A.J., Ellis, J.G. 2005. Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theor. Appl. Genet. 111:496–504.CrossRefGoogle Scholar
  24. McCartney, C.A., Somers, D.J., McCallum, B.D., Thomas, J., Humpherys, D.G., Menzies, J.G. Brown, P.D. 2005. Microsatellite tagging of leaf rust resistance gene Lr16 on wheat chromosome 2BS. Mol. Breed. 15:329–337.CrossRefGoogle Scholar
  25. McIntosh, R.A., Dubcovsky, J., Rogers, W.J., Morris, C., Xia, X.C. 2017. Catalogue of Gene Symbols for Wheat: 2017 Supplement. In: KOMUGI–Integrated Wheat Science Database. http://shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2017.pdfGoogle Scholar
  26. Nagarajan, S., Nayar, S.K., Bahadur, P., Kumar, J. 1986. Wheat Pathology and Wheat Improvement. I.A.R.I., Regional Station, Flowerdale, Shimla 171002, India. 12 p.Google Scholar
  27. Nayar, S.K., Prashar, M., Bhardwaj, S.C. 1997. Manual of current techniques in wheat rusts. Regional Station, Directorate of Wheat Research. Flowerdale, Shimla-171002, India. Res. Bull. No. 2: 32 p.Google Scholar
  28. Park, R.F. 2007. Stem rust of wheat in Australia. Austr. J. Agric. Res. 58:558–566.CrossRefGoogle Scholar
  29. Prins, R., Groenewald, J.Z., Marais, G.F., Snape, J.W., Koebner, R.M.D. 2001. AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theor. Appl. Genet. 103:618–624.CrossRefGoogle Scholar
  30. Rouse, M.N., Wanyera, R., Njau, P., Jin, Y. 2011. Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Dis. 95:762–766.CrossRefGoogle Scholar
  31. Rouse, M.N., Nava, I.C., Chao, S., Anderson, J.A., Jin, Y. 2012. Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.). Theor. Appl. Genet. 125:877–885.CrossRefGoogle Scholar
  32. Saini, R.G., Kaur, M., Singh, B., Sharma, S., Nanda, G.S., Nayar, S.K., Gupta, A.K., Nagrajan, S. 2002. Lr48 and Lr49, novel hypersensitive adult plant leaf rust resistance genes in wheat (Triticum aestivum L.). Euphytica 124:365–370.CrossRefGoogle Scholar
  33. Silva, P., Calvo-Salazar, V., Condón, F., Quincke, M., Pritsch, C., Gutiérrez, L., Herrera-Foessel, S., von Zitzewitz, J., German, S. 2015. Effects and interactions of genes Lr34, Lr68 and Sr2 on wheat leaf rust adult plant resistance in Uruguay. Euphytica 204:599–608.CrossRefGoogle Scholar
  34. Singh, R.P. 1992. Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathol. 32:835–838.CrossRefGoogle Scholar
  35. Singh, R.P., Rajaram, S. 1994. Genetics of adult plant resistance to stripe rust in ten bread wheats. Euphytica 72:1–7.CrossRefGoogle Scholar
  36. Singh, R.P., Huerta-Espino, J., Rajaram, S. 2000. Achieving near immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol. et Entomologica Hungarica 35:133–139.Google Scholar
  37. Singh, R.P., Hodson, D.P., Huerta-Espino, J., Jin, Y., Bhavani, S., Njau, P., Herrera-Foessel, S., Singh, P.K., Singh, S., Singh, V.G. 2011. The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu. Rev. Phytopathol. 49:465–481.CrossRefGoogle Scholar
  38. Spielmeyer, W., Sharp, P.J., Lagudah, E.S. 2003. Identification and validation of markers linked to broad spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci. 43:333–336.Google Scholar
  39. Stakman, E.C., Stewart, D.M., Loegering, W.Y. 1962. Identification of physiologic races of Puccinia graminis var. tritici. United States Department of Agriculture Technical Bulletin ARS E-617.Google Scholar
  40. Stubbs, R.W. 1985. Stripe rust. In: Roelfs, A.P., Bushnell, W.R. (eds), The Cereal Rusts. Vol. 2. Diseases, Distribution, Epidemiology and Control. Academic Press. Orlando, FL, USA, pp. 61–101.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • H. Khan
    • 1
  • S. C. Bhardwaj
    • 1
    Email author
  • O. P. Gangwar
    • 1
  • P. Prasad
    • 1
  • P. L. Kashyap
    • 1
  • S. Savadi
    • 1
  • S. Kumar
    • 1
  • R. Rathore
    • 1
  1. 1.ICAR - Indian Institute of Wheat and Barley ResearchFlowerdale, ShimlaIndia

Personalised recommendations