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Cereal Research Communications

, Volume 45, Issue 2, pp 248–259 | Cite as

Induction of Basal Resistance by Methyl Jasmonate against Fusarium culmorumin Bread Wheat

  • P. Motallebi
  • S. TontiEmail author
  • V. Niknam
  • H. Ebrahimzadeh
  • A. Pisi
  • P. Nipoti
  • M. Hashemi
  • A. Prodi
Article

Abstract

Fusarium culmorum is a soilborne fungal pathogen, agent of crown and root rot disease (FCRR), responsible of major economic losses in wheat plants. This host-pathogen interaction, following methyl jasmonate (MeJA) application at the beginning of the necrotrophic stage of infection, has not been previously studied at molecular level. In this study, using real-time quantitative PCR, the emerging role of MeJA in the basal resistance of two bread wheat cultivars against F. culmorum has been investigated. MeJA treatment was dispensed 6 hours after pathogen inoculation (6 hai) to detect the defense response at the beginning of the necrotrophic stage. The expression of phenylalanine ammonia-lyase (PAL), lipoxygenase (LOX), cytochrome P450 (CYP709C1) genes and of some pathogenesis related (PR) genes, including PR3, PR4 and PR9, was examined in both root and crown tissues of the susceptible wheat cultivar Falat and the tolerant cultivar Sumai3. The pathogen responsive defense genes were induced in both cultivars, with a higher level of induction in Sumai3 than in Falat. MeJA treatment reduced the symptoms in cv Falat, whereas no significant effects have been detected in cv Sumai3. In fact, MeJA treatment caused a striking difference in defense gene induction. The genetic change was present in root and crown tissues of both wheat cultivars, demonstrating a systemic signaling pathway. The chemically induced protection correlated with induction of the F. culmorum-responsive genes supports a possible role of jasmonate signaling in regulating basal resistance in wheat-F. culmorum interaction.

Keywords

Fusarium culmorum systemic acquired resistance methyl jasmonate realtime quantitative PCR Triticum aestivum 

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Induction of Basal Resistance by Methyl Jasmonate against Fusarium culmorumin Bread Wheat

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Copyright information

© Akadémiai Kiadó, Budapest 2017

Authors and Affiliations

  • P. Motallebi
    • 1
    • 2
  • S. Tonti
    • 2
    Email author
  • V. Niknam
    • 1
  • H. Ebrahimzadeh
    • 1
  • A. Pisi
    • 2
  • P. Nipoti
    • 2
  • M. Hashemi
    • 3
  • A. Prodi
    • 2
  1. 1.Department of Plant Biology, Center of Excellence in Phylogeny of Living Organisms, School of Biology, College of ScienceUniversity of TehranTehranIran
  2. 2.Department of Agricultural SciencesAlma Mater Studiorum University of BolognaBolognaItaly
  3. 3.Department of Plant PathologySeed and Plant Improvement InstituteKarajIran

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