Effect of Ozone and Antioxidants on Wheat and its Pathogen — Bipolaris sorokininana

Abstract

Tropospheric ozone (O3) adversely affects growth and productivity of crops and also influences crop–pathogen interactions. Adverse effects of O3 on crops can be mitigated by antioxidants application. In the present study through lab and field experiments impact of O3 and antioxidants treatment to wheat was assessed on growth of Bipolaris sorokiniana (BS-75 strain) pathogen responsible for Spot blotch disease, pathogenesis related (PR) proteins and chitinase content. Results showed that growth of Bipolaris was significantly higher in elevated ozone (EO3) exposed plants as compared to control plants. Antioxidants – ascorbic acid (AA), tagetes extract (T) and quercetin (Q) application on culture media and wheat plants, respectively, retarded the growth of Bipolaris sorokiniana. Among the three antioxidants minimum growth of Bipolaris was observed in AA-treated plants as compared to control plants. Reduction in chitinase activity and PR proteins content due to EO3 treatment in wheat plants was 18% and 78%, respectively, as compared to control plants. Increase in chitinase activity and PR proteins content due to antioxidants treatment in wheat plants was 45% and 60%, respectively, as compared to control plants.

References

  1. Barna, B., Fodor, J., Harrach, B.D., Pogány, M., Király, Z. 2012. The Janus face of reactive oxygen species in resistance and susceptibility of plants to necrotrophic and biotrophic pathogens. Plant Physiol. Biochem. 59:37–43.

    CAS  Article  Google Scholar 

  2. Chen, Z., Gallie, D. 2005. Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than increasing avoidance. Plant Physiol. 138:1673–1689.

    CAS  Article  Google Scholar 

  3. Chowdhury, A.K., Singh, G., Tyagi, B.S., Ojha, A., Dhar, T., Bhattacharya, P.M. 2013. Spot blotch disease of wheat–a new thrust area for sustaining productivity. J. of Wheat Res. 5:1–11.

    Google Scholar 

  4. Conklin, P.L., Barth, C. 2004. Ascorbic acid, a familiar small molecule intertwined in the response of plants to ozone pathogens, and the onset of senescence. Plant Cell Environ. 27:959–970.

    CAS  Article  Google Scholar 

  5. Cowan, M.M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12:564–582.

    CAS  Article  Google Scholar 

  6. Didyk, N.P., Blum, O.B. 2011. Natural antioxidants of plant origin against ozone damage of sensitive crops. Acta Physiol Plant. 33:25–34

    CAS  Article  Google Scholar 

  7. Ebrahim, S., Usha, K., Singh, B. 2011. Pathogenesis related (PR) proteins in plant defense mechanism. In: Mendez-Vilas, A. (ed.), Science against Microbial Pathogens: Communicating Current Research and Technological Advances. Formatex Microbiology Series. No. 3, 1, pp. 1043–1054.

  8. Elad, Y., Pertot, I. 2014. Climate change impacts on plant pathogens and plant diseases. J. Crop Improv. 28:99–139.

    CAS  Article  Google Scholar 

  9. Fuhrer, J. 2009. Ozone risk for crops and pastures in present and future climates. Naturwissenschaften 96:173–194.

    CAS  Article  Google Scholar 

  10. Ghude, S.D., Jena, C., Chate, D.M., Beig, G., Pfister, G.G., Kumar, R., Ramanathan, V. 2014. Reductions in India’s crop yield due to ozone. Geophys. Res. Lett. 41:5685–5691.

    Article  Google Scholar 

  11. Holland, M., Kinghorn, S., Emberson, L., Cinderby, S., Ashmore, M., Mills., G., Harmens, H., 2006. Development of a framework for probabilistic assessment of the economic losses caused by ozone damage to crops in Europe. CEH Project No. CO2309, New report to U.K. Department of Environment, Food and Rural Affairs under Contract 1/2/170–1/3/205.

  12. IPCC 2013. Climate change 2013: The physical science basis. In: Stocker, T.F., Qin, D., Plattner, G.K., Tignor, M., Allen, S.K., Boschung, J., Nauels, A., Xia, Y., Bex, V., Midgley, P.M. (eds.), Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press. Cambridge, UK and New York, NY, USA. 1535 p.

    Google Scholar 

  13. Manning, W.J., von Tiedemann, A. 1995. Climate change: potential effects of increased atmospheric carbon dioxide (CO2), ozone (O3), and ultraviolet B (UVB) radiation on plant diseases. Environ. Pollut. 88:219–245.

    CAS  Article  Google Scholar 

  14. Mina, U., Kumar, P., Varshney, C.K. 2010. Response of different growth stages of potato (Solanum tuberosum) to ozone stress. Phyton. 49:253–266.

    CAS  Google Scholar 

  15. Mikkelsen, B.L., Jørgensen, R.B., Lyngkjær, M.F. 2015. Complex interplay of future climate levels of CO2, ozone and temperature on susceptibility to fungal diseases in barley. Plant Pathol. 64:319–327.

    CAS  Article  Google Scholar 

  16. Morgan, W.T.J., Elson, L.A. 1934. A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamine. Biochemical J. 28:988–995.

    CAS  Google Scholar 

  17. Puckette, M.C., Tang, Y., Mahalingam, R. 2008. Transcriptomic changes induced by acute ozone in resistant and sensitive Medicago truncatula accessions. BMC Plant Biol. 8:46.

    Article  Google Scholar 

  18. Rice-Evans, C.A., Miller, N.J., Paganga, G. 1997. Antioxidant properties of phenolic compounds. Trends Plant Sci. 2:152–159.

    Article  Google Scholar 

  19. Saari, E.E. 1998. Leaf blight diseases and associated soil borne fungal pathogens of wheat in south and southeast Asia. In: Duveiller, E., Dubin, H.J., Reeves, J., McNab, A. (eds), Helminthosporium Blights of Wheat: Spot Blotch and Tan Spot. CIMMYT, Mexico D.F., pp. 37–51.

    Google Scholar 

  20. Schraudner, M., Graf, U., Langebartels, C., Sandermann, H. 1994. Ambient ozone can induce plant defence reactions in tobacco. Proc. of the Royal Society of Edinburgh. Section B. Biological Sciences 102:55–61.

    Article  Google Scholar 

  21. Shetty, N.P., Jørgensen, H.J.L., Jensen, J.D., Collinge, D.B., Shetty, H.S. 2008. Roles of reactive oxygen species in interactions between plants and pathogens. Eur. J. Plant Pathol. 121:267–280.

    CAS  Article  Google Scholar 

  22. Thalmair, M., Bauw, G., Thiel, S., Döhring, T., Langebartels, C., Sandermann, H., Jr. 1996. Ozone and ultraviolet B effects on the defense-related proteins β-1,3-glucanase and chitinase in tobacco. J. Plant Physiol. 148:222–228.

    CAS  Article  Google Scholar 

  23. Tiedemann, A.V. 1992. Ozone effects on fungal leaf diseases of wheat in relation to epidemiology. I. Necrotrophic pathogens. J. Phytopath. 134:177–186.

    Article  Google Scholar 

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Correspondence to U. Mina.

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Communicated by J. Kolmer

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Mina, U., Fuloria, A. & Aggarwal, R. Effect of Ozone and Antioxidants on Wheat and its Pathogen — Bipolaris sorokininana. CEREAL RESEARCH COMMUNICATIONS 44, 594–604 (2016). https://doi.org/10.1556/0806.44.2016.039

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Keywords

  • ozone
  • wheat
  • pathogen
  • Bipolaris sorokiniana