Carotenoids are important micronutrients required by humans for growth and development. Yellow maize among cereals possesses sufficient carotenoids, and thus, it is important to genetically dissect such traits for proper utilization in breeding programme. Twenty-one maize hybrids generated using novel inbreds with rare allele of β-carotene hydroxylase (crtRB1) that enhances kernel β-carotene, were evaluated at two diverse maize growing locations. Lutein, zeaxanthin and β-cryptoxanthin were positively correlated, while β-carotene showed negative correlation with other carotenoids. Grain yield did not show association with carotenoids. Preponderance of additive gene action was observed for lutein, zeaxanthin, β-cryptoxanthin and β-carotene. Experimental hybrids were much superior for kernel β-carotene compared to commercial hybrids. Based on SCA effects, high yielding experimental hybrids were identified for provitamin A and non-provitamin A carotenoids. These novel hybrid combinations of maize possessing rare allele of crtRB1 hold promise in maize biofortification programme to alleviate vitamin A deficiency and degenerative diseases in humans.
Babu, R., Rojas, N.P., Gao, S., Yan, J., Pixley, K. 2013. Validation of the effects of molecular marker polymorphisms in lcyE and crtRB1 on provitamin A concentrations for 26 tropical maize populations. Theor. Appl. Genet. 126:389–399.
Bouis, H., Hotz, C., McClafferty, B., Meenakshi, J.V., Pfeiffer, W. 2011. Biofortification: A new tool to reduce micronutrient malnutrition. Food Nutr. Bull. 32:S31–S40.
Buckner, B., Kelson, T.L., Robertson, D.S. 1990. Cloning of the y1 locus of maize, a gene involved in the biosynthesis of carotenoids. Plant Cell 2:867–876.
Burri, B.J. 1997. Beta-carotene and human health: A review of current research. Nutr. Res. 17:547–580.
Choudhary, M., Muthusamy, V., Hossain, F., Thirunavukkarasu, N., Pandey, N., Jha, S.K., Gupta, H.S. 2014. Characterization of β-carotene rich MAS-derived maize inbreds possessing rare genetic variation in β-carotene hydroxylase gene. Indian J. Genet. 74:620–623.
Choudhary, M., Hossain, F., Muthusamy, V., Thirunavukkarasu, N., Saha, S., Pandey, N., Jha, S.K., Gupta, H.S. 2015. Microsatellite marker-based genetic diversity analyses of novel maize inbreds possessing rare allele of β-carotene hydroxylase (crtRB1) for their utilization in β-carotene enrichment. J. Plant Biochem. Biotechnol. 25:12–20.
Dauchet, L., Amouyel, P., Dallongeville, J. 2009. Fruits, vegetables and coronary heart disease. Nat. Rev. Cardiol. 6:599–608.
Egesel, C.O., Wong, J.C., Lambert, R.J., Rocheford, T.R. 2003. Combining ability of maize inbreds for carotenoids and tocopherols. Crop Sci. 43:818–823.
Fraser, B.D., Bramley, P.M. 2004. The biosynthesis and nutritional uses of carotenoids. Prog. Lipid Res. 43:228–265.
Galobart, J., Sala, R., Rincon-Carruyo, X., Manzanilla, E.G., Vila, B., Gasa, J. 2004. Egg yolk colour as affected by saponification of different natural pigmenting sources. J. Appl. Poult. Res. 13:328–334.
Gupta, H.S., Hossain, F., Muthusamy, V. 2015. Biofortification of maize: An Indian perspective. Indian J. Genet. 75:1–22.
Hammershoj, M., Kidmose, U., Steenfeldt, S. 2010. Deposition of carotenoids in egg yolk by short-term supplement of coloured carrot (Daucus carota) varieties as forage material for egg-laying hens. J. Sci. Food Agri. 90:1163–1171.
Kljak, K., Drdic, M., Karolyi, D., Grbesa, D. 2012: Pigmentation efficiency of Croatian corn hybrids in egg production. Croatian J. Food Tech. Biotechnol. Nutr. 7:23–27.
Kurilich, A., Juvik, J.A. 1999. Quantification of carotenoid and tocopherol antioxidants in Zea mays. J. Agri. Food Chem. 47:1948–1955.
Liu, Y.Q., Davis, C.R., Schmaelzle, S.T., Rocheford, T., Cook, M.E., Tanumihardjo, S.A. 2012. β-Cryptoxanthin biofortified maize (Zea mays) increases β-cryptoxanthin concentration and enhances the color of chicken egg yolk. Poult Sci. 91:432–438.
Lokaewmanee, K., Yamauchi, K., Tsutomu, K., Saito, K. 2010. Effects on egg yolk color of paprika or paprika combined with marigold flower extracts. Italian J. Anim. Sci. 9:356–359.
Menkir, A., Liu, W., White, W.S., Maziya-Dixon, B., Rocheford, T. 2008. Carotenoid diversity in tropical-adapted yellow maize inbred lines. Food Chem. 109:521–529.
Muthusamy, V., Hossain, F., Thirunavukkarasu, N., Choudhary, M., Saha, S., Bhat, J.S., Prasanna, B.M., Gupta, H.S. 2014. Development of β-carotene rich maize hybrids through marker-assisted introgression of β-carotene hydroxylase allele. PLoS One 9:e113583
Muthusamy, V., Hossain, F., Thirunavukkarasu, N., Saha, S., Agrawal, P.K., Gupta, H.S. 2015a. Genetic variability and inter-relationship of kernel carotenoids among indigenous and exotic maize (Zea mays L.) inbreds. Cereal Res. Commun. 43:567–578.
Muthusamy, V., Hossain, F., Thirunavukkarasu, N., Pandey, N., Vishwakarma, A.K., Saha, S., Gupta, H.S. 2015b. Molecular characterization of exotic and indigenous maize inbreds for biofortification with kernel carotenoids. Food Biotechnol. 29:276–295.
Muthusamy, V., Hossain, F., Thirunavukkarasu, N., Saha, S., Gupta, H.S. 2015. Allelic variations for lycopene ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme. Cogent Food and Agric. 1:1033141.
Olson, J.A. 1989. Biological actions of carotenoids. J. Nutr. 119:94–95.
Prasanna, B.M., Pixley, K.V., Warburton, M., Xie, C. 2010. Molecular marker-assisted breeding for maize improvement in Asia. Mol. Breed. 26:339–356.
Senete, C.T., Guimaraes, P.E.D., Paes, M.C.D., Souza, J.C. 2011. Diallel analysis of maize inbred lines for carotenoids and grain yield. Euphytica 182:395–404.
Suwarno, W.B., Pixley, K.V., Palacios-Rojas, N., Kaeppler, S.M., Babu, R. 2014. Formation of heterotic groups and understanding genetic effects in provitamin A biofortified maize breeding program. Crop Sci. 54:14–24.
Vignesh, M., Hossain, F., Nepolean, T., Saha, S., Agrawal, P.K., Guleria, S.K., Prasanna, B.M., Gupta, H.S. 2012. Genetic variability for kernel β-carotene and utilization of crtRB1 3’TE gene for biofortification in maize (Zea mays L.). Indian J. Genet. 72:189–194.
Vignesh, M., Nepolean, T., Hossain, F., Singh, A.K., Gupta, H.S. 2013. Sequence variation in 3′UTR region of crtRB1 gene and its effect on β-carotene accumulation in maize kernel. J. Plant Biochem. Biotechnol. 22:401–408.
Yan, J., Kandianis, B.C., Harjes, E.C., Bai, L., Kim, H.E., Yang, X., Skinner, D.J., Fu, Z., Mitchell, S., Li, Q., Fernandez, S.M., Zaharoeva, M., Babu, R., Fu, Y., Palacios, N., Li, J., DellaPenna, D., Brutnell, T., Buckler, S.E., Warburton, L.M., Rocheford, T. 2010. Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain. Nat. Genet. 42:322–329.
Zunjare, R., Hossain, F., Muthusamy, V., Jha, S.K., Kumar, P., Sekhar, J.C., Guleria, S.K., Singh, N.K., Nepolean, T., Gupta, H.S. 2015. Genetics of resistance to stored grain weevil (Sitophilus oryzae L.) in maize. Cogent Food Agric. 1:1075934.
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Muthusamy, V., Hossain, F., Thirunavukkarasu, N. et al. Genetic Analyses of Kernel Carotenoids in Novel Maize Genotypes Possessing Rare Allele of β-carotene hydroxylase Gene. CEREAL RESEARCH COMMUNICATIONS 44, 669–680 (2016). https://doi.org/10.1556/0806.44.2016.035
- combining ability
- Zea mays