Six wheat cultivars, namely PBW 343, PBW 550 (stress susceptible), PBW 621, PBW 175 (drought tolerant), C 306 and HD 2967 (heat tolerant), were used in this study to evaluate the effect of heat and drought stress on the activities of peroxidases (POXs), diamine oxidase (DAO), polyamine oxidase (PAO) and arginine decarboxylase (ADC) and ornithine decarboxylase (ODC) in relation to contents of polyamines (PAs), lipid peroxide and lignin. High temperature (HT) elevated activities of syringaldazine peroxidase (SPX), guaiacol peroxidase (GPX) and coniferyl alcohol peroxidase (CPX) in heat tolerant cultivars while, drought stress accentuated ADC/ODC activities in drought tolerant cultivars. Both heat and drought stress enhanced activities of DAO and PAO alongwith contents of H2O2 in PBW 175 and C 306. Amongst studied POXs, SPX activity was relatively more and coincided well with lignin content under HT stress while, the levels of ADC/ODC paralleled with putrescine and spermidine contents under drought stress. Higher build up of thiobarbituric acid reactive substances in cultivars PBW 343 and PBW 550 indicated their membrane instability during both the stresses. Our results revealed that SPX mediated lignification leading to higher cell wall rigidity under heat stress and drought increased PAs involved in ROS scavenging due to presence of positive charges which can bind strongly to the negative charges in cellular components such as proteins and phospholipids and thereby stabilize the membranes under stress conditions.
Asthir, B., Duffus, C.M., Smith, R.C. Spoor, W. 2002. Diamine oxidase is involved in H2O2 production in the chalazal cells during barley grain filling. J. Exp. Bot. 53:677–682.
Asthir, B., Spoor, W., Duffus, C.M. 2004. Involvement of polyamine, diamine oxidase and polyamine oxidase in resistance of barley to Blumeria graminis f. Sp. hordei. Euphytica 136:307–312.
Birecka, H., Bitonti, A.J., McCann, P.P. 1985. Assaying ornithine and arginine decarboxylase in some plant species. Plant Physiol. 79:509–514.
Bouchereau, A., Aziz, A., Larher, F., Martin-Tanguy, J. 1999. Polyamines and environmental challenges: recent development. Plant Sci. 140:103–125.
Denness, L., McKenna, J.F., Segonzac, C., Wormit, A., Madhou, P., Bennett, M., Mansfield, J., Zipfel, C., Hamann, T. 2011. Cell wall damage-induced lignin biosynthesis is regulated by a reactive oxygen species and jasmonic acid dependent process in Arabidopsis. Plant Physiol. 156:1364–1374.
Dhillon-Grewal, R., Virk, D.S., Mangat, B.K., Basra, R.K., Basra, A.S. 1992. Polyamine levels in anthers of poly-cytoplasmic isonuclear male sterile lines of pearl millet. Bot. Bull. Acad. Sin. 33:97–100.
Duan, J.J., Li, J., Guo, S., Kang, Y. 2008. Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity. J. Plant Physiol. 165:1620–1635.
Gao, J.M., Xiao, Q., Ding, L.P., Chen, M.J., Yin, L., Li, J.Z., Zhou, S.Y., He, G.Y. 2008. Differential responses of lipid peroxidation and antioxidants in Alternanthera phioxeroides and Oryza sativa subjected to drought stress. Plant Growth Regul. 56:89–95.
Goyal, M., Asthir, B. 2010. Polyamine catabolism influences antioxidative defense mechanism in shoots and roots of five wheat genotypes under high temperature stress. Plant Growth Regul. 60:13–25.
Groppa, M.D., Benavides, M.P. 2008. Polyamines and abiotic stress: recent advance. Amino Acids 34:35–45.
Heath, R.L., Packer, L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:189–198.
Hu, W.H., Xiao, Y.A., Zeng, J.J., Hu, X.H. 2010. Photosynthesis, respiration and antioxidant enzymes in pepper leaves under drought and heat stresses. Biol. Plant. 54:761–765.
Iannone, M.F., Rosales, E.P., Groppa, M.D., Benavides, M.P. 2013. H2O2 involvement in polyamine-induced cell death in tobacco leaf discs. J. Plant Growth Regul. 32:745–757.
Kotak, S., Larkindale, J., Lee, U., von Koskull-Doring, P., Vierling, E., Scharf, K.D. 2007. Complexity of heat stress response in plants. Curr. Opin. Plant Biol. 10:310–316.
Kumar, S., Kaur, R., Kaur, N., Bhandhari, K., Kaushal, N., Gupta, K., Bains, T.S., Nayyar, H. 2011. Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress. Acta Physiol. Plant. 33:2091–2101.
Lee, T.M, Lin, Y.H. 1995. Changes in soluble and cell wall-bound peroxidase activities with growth in anoxia-treated rice (Oryza sativa L.) coleoptiles and roots. Plant Sci. 106:1–7.
Lin, C.C., Kao, C.H. 2001. Cell wall peroxidase activity, hydrogen peroxidase level and NaCl-inhibited root growth of rice seedling. Plant Soil. 230:135–143.
Liu, H.P., Dong, B.H., Zhang, Y., Liu, Z.P., Liu, Y.L. 2010. Relationship between osmotic stress and the levels of free, conjugated and bound polyamines in leaves of wheat seedlings. Plant Sci. 166:1261–1267.
Liu, J.H., Kitashiba, H., Wang, J., Ban, Y., Moriguchi, T. 2007. Polyamine and their ability to provide environmental stress tolerance to plants. Plant Biotechnol. 24:117–126.
Liu, J.H., Wang, W., Wu, H., Gong, X., Moriguchi, T. 2015. Polyamines function in stress tolerance: from synthesis to regulation Front. Plant Sci. 6:827.
Magda, P., Szalai, G., Janda, T. 2015. Speculation: Polyamines are important in abiotic stress signalling. A review. Plant Sci. 237:16–23.
Nakashima, J., Chen, F., Jackson, L., Shadle, G., Dixon, R.A. 2008. Multi-site genetic modification of monolignol biosynthesis in alfalfa (Medicago sativa): effects on lignin composition in specific cell types. New Phytol. 179:738–750.
Oktem, H.A., Eyidooan, F., Demirba, D., Bayrac, A.T., Oz, M.T., Ozgur, E., Selcuk, F., Yucel, M. 2008. Antioxidant responses of lentil to cold and drought stress. J. Plant Biochem. Biotechnol. 17:15–21.
Pathak, M.R., da Silva, J.A.T., Wani, S.H. 2014. Polyamines in response to abiotic stress tolerance through transgenic approaches. GM Crops and Food 5:87–96.
Quiroga, M., Guerrero, C., Botella, M.A., Barcelo, A., Amaya, I., Medina, M.I., Alonso, F.J., Forchetti, S.M., Tigier, H., Valpuesta, V. 2000. A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol. 122:1119–1127.
Ros-Barcelo, A., Pomar, F., Lopes-Serrano, M., Martinez, P., Pendreno, M.A. 2002. Developmental regulation of the H2O2 producing system and basic peroxidase isoenzyme in the Zinnia elegans lignifying xylem. Plant Physiol. Biochem. 40:325–332.
Savidge, R., Udagama-Randeniya, P. 1992. Cell-wall-bound coniferyl alcohol oxidase associated with lignification in conifers. Phytochem. 31:2959–2966.
Sergiev, I., Alexieva, V., Karanov, E. 1997. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. C. R. Acad. Bulg. Sci. 51:121–124.
Shu, S., Yuan, Y., Chen, J., Jin, S., Zhang, W., Tang, Y., Zhong, M., Guo, S. 2015. The role of putrescine in the regulation of proteins and fatty acids of thylakoid membranes under salt stress. Scientific Reports DOI: 10.1038/srep14390
Suzuki, N., Mittler, R. 2006. Reactive oxygen species and temperature stresses: a delicate balance between signalling and destruction. Physiol. Plant. 126:45–51.
Tao, S., Khanizadeh, S., Zhang, H., Zhang, S. 2009. Anatomy, ultrastructure and lignin distribution of stone cells in two Pyrus species, Plant Sci. 176:413–419.
Zao, F.G., Liu, Y.L. 2000. Study on determination of ADC and TGase activities. Plant Physiol. Commun. 36:442–444.
Communicated by A. Pécsváradi
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Bala, S., Asthir, B. & Bains, N.S. Syringaldazine Peroxidase Stimulates Lignification by Enhancing Polyamine Catabolism in Wheat during Heat and Drought Stress. CEREAL RESEARCH COMMUNICATIONS 44, 561–571 (2016). https://doi.org/10.1556/0806.44.2016.028
- arginine decarboxylase
- membrane stability
- ornithine decarboxylase