TaERECTA Responses to Phytohormones, Mg2+ Stress and Dehydration and Its Correlation with Stomatal Density in Bread Wheat
ERECTA is an ancient family of leucine-rich repeat receptor-like kinases (RLKs) that coordinate growth and development of plant. TaERECTA, one copy of the ERECTA homologs in wheat, was isolated from bread wheat Chinese Spring. The Ser/Thr kinase of TaERECTA was expressed in E. coli after IPTG induction and confirmed by immunoblot. TaERECTA showed higher expression in younger organs with rapid development, as well as great expression in younger spikes at booting stage. Under exogenous application of gibberellin (GA3) and abscisic acid (ABA), and Mg2+ stress, the expression of TaERECTA was largely suppressed, whereas under exogenous application of indole acetic acid (IAA) and brassinolide (BR), and dehydration stress, its expression was initially suppressed and then up-regulated. Natural variation was apparent in the relative expression of TaERECTA among 9 different bread wheat lines, and its expression level was negatively correlated with the stomatal density. These results suggested that TaERECTA could be exploitable for manipulating agronomical traits important through regulating stomata density, with potential implication for bread wheat improvement.
KeywordsTaERECTA immunoblot phytohormone Mg2+ stress dehydration stomatal density
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This work was financially supported by the open Project of the State Key Laboratory of Crop Stress Biology for Arid Areas (CSBAA2016012); a Joint PhD scholarship of the Chinese Scholarship Council; the China 111 Project (B12007) of the Ministry of Education of China; Science and Technology Innovation Team Plan of Shaanxi Province (2014KCT-25). The authors are grateful to Dr. Malcolm Hawkesford and Mr. Andrew Riche for providing the plant material of Paragon and the Watkins Collection accessions, Pippa Madgwick for the isolation of TaERECTA gene, Elizabete Carmo-Silva for collecting samples of nine wheat genotypes, Martin A. J. Parry for experiment guidance, Till Pellny for advice on qRT-PCR techniques and primers for the reference genes (TaActin and TaSand).
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