Advertisement

Cereal Research Communications

, Volume 44, Issue 3, pp 361–369 | Cite as

Assessing Genetic Diversity of Wheat Genotypes from Different Origins by SNP Markers

  • V. SpanicEmail author
  • V. Korzun
  • E. Ebmeyer
Genetics

Abstract

Genetic diversity was investigated in a set of eleven hexaploid wheat genotypes originated from CIMMYT, Turkey in comparison with some modern European mostly originated from KWS wheat breeding program using 24 wheat SNP markers. The lowest and highest genetic dissimilarities were observed between genotypes Opus and LDO 330/06, KWS Salix and LDO 330/06, respectively. Based on cluster analysis, 38 wheat genotypes were grouped in two main clusters. Although the grouping pattern is very origin heterogeneous in each group, the grouping pattern of some genotypes appeared to be associated, to some extent. Principal coordinate analysis (PCoA) was used as an alternative way of visualizing the genotypic data. The first, second and third principal components explained 17.79%, 14.39% and 12.24% of the variation, respectively. This study can also be an indicator for breeders to evolve genotypes with diverse genetic background to achieve sustainability in wheat production, to get favorable heterotic combinations in a wheat improvement program.

Keywords

genetic diversity bread wheat (Triticum aestivum L.) SNP markers 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Notes

Acknowledgements

We acknowledge KWS-Borlaug fellowship for this study and KWS LOCHOW GMBH, Germany for supporting this work.

References

  1. Abdellatif, K.F., AbouZeid, H.M. 2011. Assessment of genetic diversity of Mediterranean bread wheat using Randomly Amplified Polymorphic DNA (RAPD) markers. J. of Genetic Engineering and Biotechnol. 9:157–163.CrossRefGoogle Scholar
  2. Drikvand, R., Bihamta, M.R., Najafian, G., Ebrahimi, A. 2013. Investigation of genetic diversity among bread wheat cultivars (Triticum aestivum L.) using SSR markers. J. Agric. Sci. 5:122–129.Google Scholar
  3. Eivazi, A.R., Naghavi, M.R., Hajheidari, M., Pirseyedi, S.M., Ghaffari, M.R., Mohammadi, S.A., Majidi, I., Salekdeh, G.H., Mardi, M. 2008. Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Ann. Appl. Biol. 152:81–91.Google Scholar
  4. FAO 2011. Food and agricultural commodities production. http://faostat.fao.org/site/339/default.aspx.Google Scholar
  5. Fu, Y.B., Peterson, G.W., Yu, J.K., Gao, L., Jia, J., Richards, K.W. 2006. Impact of plant breeding on genetic diversity of the Canadian hard red spring wheat germplasm as revealed by ESTderived SSR markers. Theor. Appl. Genet. 112:1239–1247.CrossRefGoogle Scholar
  6. Gorji, A.H., Zolnoori, M. 2011. Genetic diversity in hexaploid wheat genotypes using microsatellite markers. Asian J. of Biotechnol. 3:368–377.CrossRefGoogle Scholar
  7. Gower, J.C. 1966. Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 53:325–338.CrossRefGoogle Scholar
  8. He, C., Holmes, J., Anthony, J. 2014. SNP genotyping: the KASP assay. Methods Mol. Biol. 1145:75–86.CrossRefGoogle Scholar
  9. Huang, X.Q., Hsam, S.L.K., Zeller, F.J., Wenzel, G., Mohler, V. 2000. Molecular mapping of wheat powdery mildew resistance gene Pm24 and marker validation for molecular bredding. Theor. Appl. Genet. 101:407–414.CrossRefGoogle Scholar
  10. Landjeva, S., Korzun, V., Ganeva, G. 2006. Evaluation of genetic diversity among Bulgarian winter wheat (Triticum aestivum L.) varieties during the period 1925–2003 using microsatellites. Genet. Resour. Crop Evol. 53:1605–1614.CrossRefGoogle Scholar
  11. Liu, K., Muse, S.V. 2005. Powermarker: An integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129.CrossRefGoogle Scholar
  12. Pandey, A., Khan, M.K., Thomas, G., Hakki, E.E., Kayis, S.A., Hamurcu, M., Gezgin, S., Topal, A., Akkaya, M.S. 2015. Estimation of Indian and Turkish hexaploid wheat population structure employing molecular markers. Not. Bot. Horti. Agrobo. 43:70–78.CrossRefGoogle Scholar
  13. Prasad, B., Babar, M.A., Xu, X.Y., Bai, G.H., Klatt, A.R. 2009. Genetic diversity in the U.S. hard red winter wheat cultivars as revealed by microsatellite markers. Crop and Pasture Sci. 60:16–24.Google Scholar
  14. Rafalski, A. 2002. Applications of single nucleotide polymorphisms in crop genetics. Curr. Opin. Plant Biol. 5:94–100.CrossRefGoogle Scholar
  15. Ren, J., Sun, D., Chen, L., You, F.M., Wang, J., Peng, Y., Nevo, E., Sun, D., Luo, M.C., Peng, J. 2013. Genetic diversity revealed by single nucleotide polymorphism markers in a worldwide germplasm collection of durum wheat. Int. J. Mol. Sci. 14:7061–7088.CrossRefGoogle Scholar
  16. Sears, E.R. 1954. The aneuploids of common wheat. Bull. 572. University of Missouri Agricultural. Columbia, SC, USA.Google Scholar
  17. Semagn, K., Babu, R., Hearne, S., Olsen, M. 2014. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): Overview of the technology and its application in crop improvement. Mol. Breed. 33:1–14.CrossRefGoogle Scholar
  18. Song, Q.J., Shi, J.R., Singh, S., Fickus, E.W., Costa, J.M., Lewis, J., Gill, B.S., Ward, R., Cregan, P.B. 2005. Development and mapping of microsatellite (SSR) markers in wheat. Theor. Appl. Genet. 110:550–556.CrossRefGoogle Scholar
  19. Wright, S. 1978. Evolution and Genetics of Populations, Variability within and among Natural Populations, 4th edn. The University of Chicago Press. Chicago, USA.Google Scholar
  20. Würschum, T., Langer, S.M., Longin, C.F.H., Korzun, V., Akhunov, E., Ebmeyer, E., Schachschneider, R., Schacht, J., Kazman, E., Reif, J.C. 2013. Population structure, genetic diversity and linkage disequilibrium in elite winter wheat assessed with SNP and SSR markers. Theor. Appl. Genet. 126:1477–1486.CrossRefGoogle Scholar
  21. Zhu, Y., Chen, H., Fan, J., Wang, Y., Li, Y., Chen, J., Fan, J.X., Yang, S., Hu, L., Leung, H., Mew, T.W., Teng, P.S., Wang, Z., Mundt, C.C. 2000. Genetic diversity and disease control in rice. Nature 406:718–722.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department for Breeding and Genetics of Small Cereal Crops, Agricultural Institute OsijekOsijekCroatia
  2. 2.KWS LOCHOW GMBH, Ferdinand von-Lochow-Straße 5BergenGermany

Personalised recommendations