Cereal Research Communications

, Volume 44, Issue 3, pp 381–392 | Cite as

Hydroponic Culturing Upregulates Sucrose and Glutamine Metabolism by Enhancing Their Utilization via Intermediates of Aerobic Pathway in Wheat

  • B. Kaur
  • B. AsthirEmail author


Two wheat genotypes were grown in hydroponic culture, containing 4 mM KNO3, NH4Cl and NH4NO3. Activities of N metabolizing enzymes, aminotransferases, carbohydrate and TCA cycle enzymes were analyzed along with protein, amino acid, N, sugar content and growth parameters in shoot and root. After 12 days, the size of shoot and root system decreased significantly when plants were supplied with NH4Cl as exclusive N source. Under NH4NO3 growth parameters, N and carbon metabolism were elevated as compared to NH4Cl but less than KNO3 source indicating inhibition of NH4+ toxicity by NO3 uptake. Our results suggested that GDH, aminotransferases and PEPC play an important role in ammonium detoxification by its incorporation into amino acids. Thus, the morphologic differences among plants growing in NH4+ or NO3 nutrition confirm the hypothesis that N source determines the growth habit of plant in wheat by modulating the endogenous levels of protein and sugar content.


nitrogen carbon hydroponics wheat genotypes 





glutamate dehydrogenase


glutamate synthase


glutamate oxaloacetate transaminase


glutamate pyruvate transaminase


glutamine synthetase


isocitrate dehydrogenase


malate dehydrogenase


nitrate reductase


phosphoenolpyruvate carboxylase


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Hydroponic Culturing Upregulates Sucrose and Glutamine Metabolism by Enhancing Their Utilization via Intermediates of Aerobic Pathway in Wheat


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Authors and Affiliations

  1. 1.Department of BiochemistryPunjab Agricultural UniversityLudhiana, PunjabIndia

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