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Cereal Research Communications

, Volume 44, Issue 1, pp 131–140 | Cite as

Spray-dried Powder of Bacillus megaterium for Control of Rice Sheath Blight Disease: Formulation Protocol and Efficacy Testing in Laboratory and Greenhouse

  • A. ChumthongEmail author
  • R. Wiwattanapatapee
  • H. Viernstein
  • A. Pengnoo
  • M. Kanjanamaneesathian
Physiology

Abstract

A spray-dried powder containing Bacillus megaterium was developed and tested for control of rice sheath blight disease in the greenhouse. The formulation consisted of 20 ml of an endospore suspension of B. megaterium, 20% w/v of skim milk powder and 1.25% w/v of polyvinyl pyrrolidone k-90, that were mixed and spray dried at 120 °C. The powder displayed good physical characteristics, such as a low-moisture content and a high solubility in water. Bacterial viability in the powder was 3.5±0.1 × 1011 cfu/g after production and remained relatively stable (at 2.2±0.1 × 1010 cfu/g) after 12 months of storage at room temperature. In the laboratory, a 0.1% (w/v) aqueous solution of the formulation was effective in inhibiting the mycelia growth of Rhizoctonia solani (98.5±0.1% inhibition). Under greenhouse conditions, a 0.1% (w/v) aqueous solution applied by either spraying 1 day before inoculating R. solani or spraying 1, 7 and 15 days after inoculation of rice plants with R. solani was more effective in suppressing sheath blight disease than the blank formulation but was less effective than a chemical fungicide control.

Keywords

Bacillus megaterium bio-pesticide formulation rice disease spray-dried powder 

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Notes

Acknowledgement

We would like to thank Dr. Brian Hodgson for assistance with the English.

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • A. Chumthong
    • 1
    Email author
  • R. Wiwattanapatapee
    • 2
  • H. Viernstein
    • 3
  • A. Pengnoo
    • 4
  • M. Kanjanamaneesathian
    • 5
  1. 1.Department of Agricultural Technology, Faculty of Agricultural TechnologySongkhla Rajabhat UniversityMuang, SongkhlaThailand
  2. 2.Department of Pharmaceutical Technology, Faculty of Pharmaceutical SciencesPrince of Songkla UniversityHat Yai, SongkhlaThailand
  3. 3.Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life SciencesUniversity of ViennaViennaAustria
  4. 4.Department of Earth Sciences, Faculty of Natural ResourcesPrince of Songkla UniversityHatYai, SongkhlaThailand
  5. 5.Plant Production Technology Program, Faculty of Animal Science and Agricultural TechnologySilpakorn University, Phetchaburi IT campusCha-Am, PhetchaburiThailand

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