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Cereal Research Communications

, Volume 43, Issue 4, pp 638–648 | Cite as

Using Markers and Field Evaluation to Identify the Source of Eyespot Resistance Gene Pch1 in the Collection of Wheat Breeding Lines

  • M. KwiatekEmail author
  • H. Wiśniewska
  • Z. Kaczmarek
  • M. Korbas
  • M. Gawłowska
  • M. Majka
  • K. Pankiewicz
  • J. Danielewicz
  • J. Belter
Pathology

Abstract

Pch1 gene translocated from Aegilops ventricosa provides effective resistance to eyespot in wheat. To track the Pch1 gene introgression, we investigated 372 genotypes obtained from various breeding programs using endopeptidase EpD1b marker, sequence-tagged-site (STS) marker XustSSR2001-7DL, and the score of infection index (K-index) evaluated after in vivo inoculation test. These genotypes were divided into three groups with 136, 124 and 112 genotypes for the field test lasting three years. In 2011, the mean K-index was 0.81, while 2012 and 2013 the mean K-indexes were 1.60 and 1.46, respectively. Both marker results indicated that 18 genotypes possessed Pch1 gene. Statistical analysis of the level of K-index showed that these 18 genotypes were resistant to eyespot, which verified the proper assignment of wheat genotypes with Pch1 gene based on the marker data. Thus, the endopeptidase and XustSSR2001-7DL are useful for identifying sources of eyespot resistance gene Pch1 in wheat breeding program.

Keywords

endopeptidase eyespot disease inoculation tests isozyme STS marker Triticum aestivum 

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Notes

Acknowledgement

This work was financed by the Ministry of Agronomy and Rural Development (HOR hn - 801 - 11/13).

Supplementary material

42976_2015_43040638_MOESM1_ESM.pdf (242 kb)
Using Markers and Field Evaluation to Identify the Source of Eyespot Resistance Gene Pch1 in the Collection of Wheat Breeding Lines

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Copyright information

© Akadémiai Kiadó, Budapest 2015

Authors and Affiliations

  • M. Kwiatek
    • 1
    Email author
  • H. Wiśniewska
    • 1
  • Z. Kaczmarek
    • 1
  • M. Korbas
    • 2
  • M. Gawłowska
    • 1
  • M. Majka
    • 1
  • K. Pankiewicz
    • 1
  • J. Danielewicz
    • 2
  • J. Belter
    • 1
  1. 1.Institute of Plant Genetics Polish Academy of SciencePoznańPoland
  2. 2.Institute of Plant ProtectionNational Research InstitutePoznańPoland

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