Cereal Research Communications

, Volume 43, Issue 4, pp 649–660 | Cite as

Mapping QTL for Resistance against Pyrenophora tritici-repentis in Wheat

  • M. R. SimónEmail author
  • F. M. Ayala
  • M. V. Moreno
  • U. Lohwasser
  • A. Börner
Open Access


Tan spot, caused by the fungus Pyrenophora tritici-repentis (Died.) Drechs is an important foliar disease of wheat (Triticum aestivum L.). From a set of phenotypically and molecularly characterized set of Argentinean isolates, two isolates H0019 and H0120 which do not correspond to known races of the pathogen were selected. Segregation for resistance among a set of recombinant inbred lines bred from the cross ‘W7984’ × cv. ‘Opata 85’ was used to identify the basis for resistance at the seedling stage, against those fungal isolates (H0019 and H0120), across three independent environments. On the basis of the mean performance across all three environments, a QTL against chlorosis located on the 6AS and linked to the RFLP locus Xksuh4c was significant for both isolates (with a LOD of 3.76 for isolate H0019 and 5.87 for H0120).


tan spot resistance races QTL 



polymerase chain reaction


recombinant inbreed lines


growth stage


log likelihood ratio

Supplementary material

42976_2015_43040649_MOESM1_ESM.pdf (299 kb)
Mapping QTL for Resistance against Pyrenophora tritici-repentis in Wheat


  1. Ali, S., Francl. L.J. 2003. Population race structure of Pyrenophora tritici-repentis prevalent on wheat and noncereal grasses in the great plains. Plant Dis. 87:418–422.PubMedCrossRefPubMedCentralGoogle Scholar
  2. Ali, S., Gurung, S., Adhikari, T.B. 2010. Identification and characterization of novel isolates of Pyrenophora tritici-repentis from Arkansas. Plant Dis. 94:229–235.PubMedCrossRefPubMedCentralGoogle Scholar
  3. Anderson, J.A., Effertz, R.J. Faris, J.D., Francl, L.J., Meinhardt, S.W., Gill, B.S. 1999. Genetic analysis of sensitivity to Pyrenophora tritici-repentis necrosis inducing toxin in durum and common wheat. Phytopathol. 89:293–297.CrossRefGoogle Scholar
  4. Andrie, R.M., Pandelova, I., Ciuffetti, L.M. 2007. A combination of phenotypic and genotypic characterization strengthens Pyrenophora tritici-repentis race identification. Phytopathol. 97:694–701CrossRefGoogle Scholar
  5. Arseniuk, E., Czembor, P.C., Czaplicki, A., Song, Q., Cregan, P.B., Hoffman, D.L., Ueng, P.P. 2004. QTL controlling partial resistance to Stagonospora nodorum leaf blotch in winter wheat cultivar Alba. Euphytica 137:225–231.CrossRefGoogle Scholar
  6. Bockus, W.W., Su, Z., Garret, K.A. Gill, B.S., Stack, J., Fritz, A.K., Roozeboom, K.L., Martin, T.J. 2007. Number of experiments needed to determine wheat disease phenotypes for four wheat diseases. Plant Dis. 91:103–108.PubMedCrossRefPubMedCentralGoogle Scholar
  7. Effertz, R.J., Anderson J.A., Francl, L.J. 2001. Restriction fragment length polymorphism mapping of resistance to two races of Pyrenophora tritici-repentis in adult and seedling wheat. Phytopathol. 91:572–578.CrossRefGoogle Scholar
  8. Effertz, R.J., Meinhardt, J.A., Anderson, J.A, Jordahl, J.G., Francl, L.J. 2002. Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat. Phytopathol. 92:527–533.CrossRefGoogle Scholar
  9. Faris, J.D., Anderson, J.A.,Francl, L.J., Jordahl, J.G. 1996. Chromosomal location of a gene conditioning insensitivity in wheat to a necrosis-inducing culture filtrate from Pyrenophora tritici-repentis in wheat. Phytopathol. 86:459–463CrossRefGoogle Scholar
  10. Faris, J.D., Anderson, J.A., Francl, L.J., Jordahl, J.G. 1997. RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat. Theor. Appl. Genet. 94:98–103.PubMedCrossRefPubMedCentralGoogle Scholar
  11. Faris, J.D. Liu, Z., Xu, S. 2013. Genetics of tan spot resistance. Theor. Appl. Genet. 126:2197–2217.PubMedCrossRefPubMedCentralGoogle Scholar
  12. Ferreira, M.E., Grattapaglia, D. 1998. Introdução ao Uso de Marcadores Moleculares em Análise Genética (Introduction to the use of molecular markers in genetic analysis). 3ª ediçã. M.A. Embrapa, Brasilia. 220 p. (in Portuguese)Google Scholar
  13. Friesen, T.L., Faris, J.D. 2004. Molecular mapping of resistance to Pyrenophora tritici-repentis race 5 and sensitivity to Ptr Tox B in wheat. Theor. Appl. Genet. 109:464–471.PubMedCrossRefPubMedCentralGoogle Scholar
  14. Gamba, F.M., Lamari, L., Brule-Babel, A. 1998. Inheritance of race-specific necrotic and chlorotic reactions induced by Pyrenophora tritici-repentis in hexaploid wheats. Can. J. Plant Pathol. 20:401–407.CrossRefGoogle Scholar
  15. Ganal, M.W, Röder. M.S. 2007. Microsatellite and SNP markers in wheat breeding. In: Varshney, R.K., Tuberosa, R. (eds.), Genomics Assisted Crop Improvement: Vol. 2: Genomics Applications in Crops. Springer. Dordrecht, The Netherlands. pp. 1–24.Google Scholar
  16. Genstat 2008. 3rd edition. VSN International, Hemel Hempstead, UK.Google Scholar
  17. Gurung, S., Mamidi, S., Bonman, J.M. Jackson, E.W., del Rio, L.E., Acevedo, M. Mergoum, M., Adhikari, T.B. 2011. Identification of novel genomic regions associated with resistance to Pyrenophora tritici-repentis races 1 and 5 in spring wheat landraces using association analysis. Theor. Appl. Gen. 123:1029–1041. Hosford, R.M. Jr. 1971. A form of Pyrenophora tricosthoma pathogenic to wheat and other grasses. Phytopathol. 61:28–32.Google Scholar
  18. Hosford, R.M. Jr. 1982. Tan spot developing knowledge 1902–1981. In: Tan Spot of Wheat and Related Diseases Workshop. Horsford, R.M., Jr. (ed.), North Dakota State University. Fargo, USA. pp. 1–5.Google Scholar
  19. Hosford, R.M. Jr., Busch, R.H. 1974. Losses in wheat caused by Pyrenophora trichostoma and Leptosphaeria avenaria f. sp. triticea. Phytopathol. 64:184–187.Google Scholar
  20. Kohli, M., Mehta, Y., Ackerman de, M.D. 1992. Spread of tan spot in the Southern Cone Region of South America. In: Francl, L., Krupinsky, J., Mc Mullen, M. (eds), Advances in Tan Spot. Proc. Second Int. Tan Spot Workshop North Dakota State University. Fargo, ND, USA. pp. 86–90.Google Scholar
  21. Lamari, L., Bernier, C.C. 1989a. Toxin of Pyrenophora tritici-repentis: Host specificity, significance in disease, and inheritance of host reaction. Phytopathol. 79:740–744.CrossRefGoogle Scholar
  22. Lamari, L., Bernier, C.C. 1989b. Virulence of isolates of Pyrenophora tritici-repentis on 11 wheat cultivars and cytology of the differential host reaction. Can. J. Plant Pathol. 11:284–290CrossRefGoogle Scholar
  23. Lamari, L., Bernier, C.C. 1991. Genetics of tan necrosis and extensive chlorosis in tan spot of wheat caused by Pyrenophora tritici-repentis. Phytopathol. 81:1092–1095.CrossRefGoogle Scholar
  24. Lamari, L., Strelkov, S.E. Yahyaoui, A., Orabi, J., Smith, R.B. 2003. The identification of two new races of Pyrenophora tritici-repentis from the host centre of diversity confirms a one-to one relationship in tan spot of wheat. Phytopathol. 93:391–396.CrossRefGoogle Scholar
  25. Lepoint, P., Renard, E. Legreve, E. Duveiller, E., Maraite, H. 2010. Genetic diversity of the mating type and toxin production genes in Pyrenophora tritici-repentis. Phytopathol. 100:474–483.CrossRefGoogle Scholar
  26. Martinez, J.P., Oesch, N.W., Ciuffetti, L.M. 2004. Characterization of the multiple- copy host-selective toxin gene, ToxB, in pathogenic and nonpathogenic isolates of Pyrenophora tritici-repentis. Mol. Plant Microbe Interactions 17:467–474.CrossRefGoogle Scholar
  27. Moreno, M.V., Stenglein, S., Perello, A.E., Balatti, P. 2008. Pathogenic and genetic diversity of isolates of Pyrenophora tritici-repentis causing tan spot of wheat in Argentina. Eur. J. Plant Pathol. 122:239–252.CrossRefGoogle Scholar
  28. Moreno, M.V., Stenglein, S., Perelló, A.E. 2015. Distribution of races and Tox genes in Pyrenophora tritici-repentis isolates from wheat in Argentina. Tropical Plant Pathol, 40:141–146.CrossRefGoogle Scholar
  29. Nelson, J.C. 1997. QGene: Software for mapping-based genomic analysis and breeding. Mol. Breed. 3:239–245.CrossRefGoogle Scholar
  30. Noriel, A.J., Sun, X., Bockus, W., Bai, G. 2011. Resistance to tan spot and insensitivity to Ptr ToxA in wheat. Crop Sci. 51:1059–1067CrossRefGoogle Scholar
  31. Odvody, G., Boosalis, M.G.. 1982. An efficient conidial production technique for Pyrenophora trichostoma. In: Tan Spot of Wheat and Related Disease Workshop. Hosford, R. M., Jr. (ed.), North Dakota State University, Fargo, USA. 116 p.Google Scholar
  32. Orolaza. N.P., Lamari, L., Balance, G.M. 1995. Evidence of a host-specific chlorosis toxin from Pyrenophora tritici-repentis, the causal agent of tan spot of wheat. Phytopathol. 85:1282–1287.CrossRefGoogle Scholar
  33. Patel, J.S., Mamidi, S., Michael Bonman, J., Adhikari, T.B. 2013. Identification of QTL in spring wheat associated with resistance to a novel isolate of Pyrenophora tritici-repentis. Crop Sci. 53:453–483.CrossRefGoogle Scholar
  34. Perelló, A.E., Moreno, M.V., Simón, M.R., Sisterna, M. 2003. Tan spot of wheat infection at different stage of crop development and inoculums type. Crop Prot. 22:157–169.CrossRefGoogle Scholar
  35. Platt, H., Morrall, R.A., Gruen, H.E. 1977. The effects of substrate, temperature and photoperiod on conidiation of Phyrenophora tritici-repentis. Can. J. Bot. 55:254:259.CrossRefGoogle Scholar
  36. Rees, R.G., Mayer, R.J., Platz, G.J. 1981. Yield losses in wheat from yellow spot: A disease-loss relationship derivated from single tillers. Aust. J. Agr. Res. 32:851–859.CrossRefGoogle Scholar
  37. Rees, R.G., Platz, G.J. 1983. Effects of yellow spot on wheat: Comparison of epidemics at different stages of crop development. Aust. J. Agric. Res. 4:39–46.CrossRefGoogle Scholar
  38. Simón, M.R., Ayala, F., Terrile, I., Golik, S., Perelló, A., Cordo, C., Chidichimo, H. 2011. Integrated foliar disease management to prevent yield loss in Argentinian wheat production. Agron. J. 103:1441–1451.CrossRefGoogle Scholar
  39. Singh, P.K., Gonzalez-Hernandez, J.L., Mergoum, M., Ali, S., Adhikari, T.B., Kianian, S.F., Elias, E.M., Hughes, G.R. 2006. Identification and molecular mapping of a gene conferring resistance to Pyrenophora tritici-repentis race 3 in tetraploid wheat. Phytopathol. 96:885–889.CrossRefGoogle Scholar
  40. Singh, P.K., Mergoum, M., Gonzalez-Hernandez, J.L., Ali, S., Adhikari, T.B., Kianian, S.F., Elias, E.M. Hughes, G.R. 2008. Genetics and molecular mapping of resistance to necrosis inducing race 5 of Pyrenophora tritici-repentis in tetraploid wheat. Mol. Breeding 21:293–304.CrossRefGoogle Scholar
  41. Stenglein, S.A., Balatti, P.A. 2006. Genetic diversity of Phaeoisariopsis griseola in Argentina as revealed by pathogenic and molecular markers. Physiol. and Mol. Plant Pathol. 68:158–167.CrossRefGoogle Scholar
  42. Strelkov, S.E., Lamari, L. 2003 Host-parasite interaction in tan spot Pyrenophora tritici-repentis of wheat. Can. J. Plant Pathol. 25:339–349.CrossRefGoogle Scholar
  43. Strelkov, S.E., Kowatsch, R.F., Balance, G.M., Lamari, L. 2006. Characterization of the ToxB gene from North America and Canadian isolates of Pyrenophora tritici-repentis. Physiol. and Mol. Plant Pathol. 67:164–170.CrossRefGoogle Scholar
  44. Sun,X.C., Bockus, W., Bai, G.H. 2010. Quantitative trait loci for resistance to Pyrenophora tritici-repentis race 1 in Chinese wheat. Phytopathol. 100:468–473.CrossRefGoogle Scholar
  45. Tadesse, W., Hsam, S.L.K., Wenzel, G., Zeller, F.J. 2006a. Identification and monosomic analysis of tan spot resistance and chromosomal location of a resistance gene in the cultivar Salamouni. Plant Breed. 125:318–322.CrossRefGoogle Scholar
  46. Tadesse, W., Hsam, S.L.K., Zeller, F.J. 2006b. Evaluation of common wheat cultivars for tan spot (Pyrenophora tritici-repentis) in synthetic wheat lines. Theor. Appl. Genet. 14:885–862.Google Scholar
  47. Tadesse, W., Schmolke, M., Mohler, V., Wenzel, G., Hsam, S.L.K., Zeller, F.J. 2007. Molecular mapping of resistance genes to tan spot (Pyrenophora tritici-repentis) in synthetic wheat lines. Theor. Appl. Genet. 14:885–862.Google Scholar
  48. Van Deynze, A.E., Dubcovsky, J., Gill, K.S., Nelson, J.C., Sorrells, M.E., Dvořák, J., Gill, B.S., Lagudah, E.S., McCouch, S.R., Appels, R. 1995. Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome 38:45–49.CrossRefGoogle Scholar
  49. Wiese, M.V. 1987. Tan spot (yellow leaf spot). In: Wiese, M.V. (ed.), Compendium of wheat diseases. The Am. Phytopathol. Soc., APS Press. St. Paul, MN, USA. pp. 42–43.Google Scholar
  50. Zadoks, J.C., Chang, T.T., Konzak, C.F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415–421.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • M. R. Simón
    • 1
    Email author
  • F. M. Ayala
    • 1
    • 4
  • M. V. Moreno
    • 2
  • U. Lohwasser
    • 3
  • A. Börner
    • 3
  1. 1.Cerealicultura, Facultad de Ciencias Agrarias y ForestalesUniversidad Nacional de La PlataLa PlataArgentina
  2. 2.CONICETLaboratorio de Biología Funcional y Biotecnología (BIOLAB)-CEBB-CONICET, Cátedra de Microbiología Agrícola, Facultad de Agronomía de Azul, UNCPBAArgentina
  3. 3.Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)GaterslebenGermany
  4. 4.Instituto de Agrobiotecnología de Rosario (INDEAR)RosarioArgentina

Personalised recommendations