Advertisement

Acta Biologica Hungarica

, Volume 68, Issue 4, pp 428–442 | Cite as

Establishment of an Efficient Plant Regeneration Culture Protocol and Achievement of Successful Genetic Transformation in Jatropha curcas L.

  • Ying LiuEmail author
  • Guoxuan Liu
  • Yali Yang
  • Sufang Niu
  • Fuguang Yang
  • Shaoxia Yang
  • Jianian Tang
  • Jianping Chen
Article

Abstract

An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5–120 mg/L) of TDZ for short durations (5–80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium–mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants.

Keywords

Jatropha curcas Petiole explants Plant regeneration Genetic transformation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adebowale, K. O., Adedire, C. O. (2006) Chemical composition and insecticidal properties of the underutilized Jatropha curcas seed oil. Afr. J. Biotechnol. 5, 901–906.Google Scholar
  2. 2.
    Attaya, A. S., Geelen, D., Belal, A. E. H. (2012) Progress in Jatropha curcas tissue culture. Am-Eurasian J. Sustain. Agric. 6, 6–13.Google Scholar
  3. 3.
    Deore, A. C., Johnson, T. S. (2008) High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.: an important biodiesel plant. Plant Biotech Rep. 2, 10–15.CrossRefGoogle Scholar
  4. 4.
    Hamilton, C. M., Frary, A., Lewis, C. (1996) Stable transfer of intact high molecular weight DNA into plant chromosomes. Proc. Natl. Acad. Sci. 93, 9975–9979.CrossRefGoogle Scholar
  5. 5.
    Höfgen, R., Willmitzer, L. (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res. 16, 9877.CrossRefGoogle Scholar
  6. 6.
    Jaganath, B., Subramanyam, K., Mayavan, S. (2014) An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting. Protoplasm. 251, 591–601.CrossRefGoogle Scholar
  7. 7.
    Jefferson, R. A. (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5, 387–405.CrossRefGoogle Scholar
  8. 8.
    Jefferson, R. A., Kavanagh, T. A., Bevan, M. W. (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. E. M. B. O. J. 6, 3901–3907.Google Scholar
  9. 9.
    Liberalino, A. A. A., Bambirra, E. A., Moraes-Santos, T. (1988) Jatropha curcas L. seeds: chemical analysis and toxicity. Arq. Biol. Technol. 31, 539–550.Google Scholar
  10. 10.
    Khemkladngoen, N., Cartagena, J. A., Fukui, K. (2011) Physical wounding-assisted Agrobacteriummediated transformation of juvenile cotyledons of a biodiesel-producing plant, Jatropha curcas L. Plant Biotechnol. Rep. 5, 235–243.CrossRefGoogle Scholar
  11. 11.
    Kumar, N., Anand, K. G. V., Reddy, M. P. (2011) In vitro regeneration from petiole explants of nontoxic Jatropha curcas. Ind. Crops Prod. 33, 146–151.CrossRefGoogle Scholar
  12. 12.
    Kumar, N., Reddy, M. P. (2012) Thidiazuron (TDZ) induced plant regeneration from cotyledonary petiole explants of elite genotypes of Jatropha curcas: a candidate biodiesel plant. Ind. Crops Prod. 39, 62–68.CrossRefGoogle Scholar
  13. 13.
    Liberalino, A. A. A., Bambirra, E. A., Moraes-Santos, T. (1988) Jatropha curcas L. seeds: chemical analysis and toxicity. Arquivos de biologia e tecnologi. 31, 539–550.Google Scholar
  14. 14.
    Liu, Y., Lu, J., Zhu, H. (2016) Efficient culture protocol for plant regeneration from cotyledonary petiole explants of Jatropha curcas L. Biotechnol. Biotec. Eq. 30, 907–914.CrossRefGoogle Scholar
  15. 15.
    Liu, Y., Tong, X., Hui, W. (2015) Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees of Jatropha curcas L. Biotechnol. Biotec. Eq. 29, 479–488.CrossRefGoogle Scholar
  16. 16.
    Liu, Y., Yin, X. G., Zhu, H. B. (2016) An efficient protocol for inducing regeneration in physic Nut (Jatropha curcas L.). Bangladesh J. Botan. 45, 87–93.Google Scholar
  17. 17.
    Liu, Y., Yu, L., Fu, Y. L. (2012) Development of an in vitro grafting method for the enhancement of growth of isolated shoots and buds in soybean (Glycine max L.). Biomed. Eng. Biotechnol. 1, 1003–1006.Google Scholar
  18. 18.
    Liu, Y., Yu, L., Zhang, Q. (2013) High concentration short duration treatment of benzyladenine stimulates adventitious bud regeneration from hypocotyl explants in soybean. Adv. Mater. Res. 647, 331–337.CrossRefGoogle Scholar
  19. 19.
    Liu, Z. L., Liu, Y., Yang, Y. S. (2016) An Agrobacterium - mediated genetic transformation method of Jatropha curcas. Patent filed China and PCT. Authorization No. ZL201410275476.6, 2016-05-25. (in Chinese).Google Scholar
  20. 20.
    Lodhi, M. A., Ye, G. N., Weeden, N. F. (1994) A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Bio. Rep. 12, 6–13.CrossRefGoogle Scholar
  21. 21.
    Lu W. (2003) Induction of callus from Jatropha curcas and rapid propagation. Chin. J. Appl. Environ. Biol. 9, 127–130.Google Scholar
  22. 22.
    Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.CrossRefGoogle Scholar
  23. 23.
    Ötvös, K., Pasternak, T. P., Miskolczi, P. (2005) Nitric oxide is required for, and promotes auxinmediated activation of, cell division and embryogenic cell formation but does not influence cell cycle progression in alfalfa cell cultures. The Plant J. 43, 849–860.CrossRefGoogle Scholar
  24. 24.
    Petri, C., Wang, H., Alburquerque, N. (2008) Agrobacterium-mediated transformation of apricot (Prunus armeniaca L.) leaf explants. Plant Cell Rep. 27, 1317–1324.CrossRefGoogle Scholar
  25. 25.
    Petřivalský, M., Vaníčková, P., Ryzí, M. (2012) The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts. Plant Cell Tiss. Org. Cult. 108, 237–249.CrossRefGoogle Scholar
  26. 26.
    Wei, Q., Lu, W. D., Liao, Y. (2004) Plant regeneration from epicotyl explant of Jatropha curcas. J. Plant Physiol. Mol. Biol. 30, 475–478.Google Scholar
  27. 27.
    Zhou, L., Ni, E., Yang, J. (2013) Rice OsGL1-6 is involved in leaf cuticular wax accumulation and drought resistance. PloS One 8, e65139.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2017

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ying Liu
    • 1
    Email author
  • Guoxuan Liu
    • 1
  • Yali Yang
    • 2
  • Sufang Niu
    • 3
  • Fuguang Yang
    • 4
  • Shaoxia Yang
    • 1
  • Jianian Tang
    • 1
  • Jianping Chen
    • 5
  1. 1.Department of Biotechnology, Faculty of Agricultural ScienceGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  2. 2.Department of Basic Medicine, School of MedicineJiaying UniversityMeizhou, GuangdongP.R. China
  3. 3.Department of Marine Biology, Fisheries CollegeGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  4. 4.Faculty of Mathematics and Computer Science, College of Software EngineeringGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China
  5. 5.Department of Food Science and Engineering, College of Food Science and TechnologyGuang Dong Ocean UniversityZhanjiang, GuangdongP.R. China

Personalised recommendations