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Establishment of an Efficient Plant Regeneration Culture Protocol and Achievement of Successful Genetic Transformation in Jatropha curcas L.

Acta Biologica Hungarica volume 68, pages 428–442 (2017)Cite this article

Abstract

An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5–120 mg/L) of TDZ for short durations (5–80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium–mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants.

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Author information

Affiliations

  1. Department of Biotechnology, Faculty of Agricultural Science, Guang Dong Ocean University, Zhanjiang, Guangdong, P.R. China

    Ying Liu, Guoxuan Liu, Shaoxia Yang & Jianian Tang

  2. Department of Basic Medicine, School of Medicine, Jiaying University, Meizhou, Guangdong, P.R. China

    Yali Yang

  3. Department of Marine Biology, Fisheries College, Guang Dong Ocean University, Zhanjiang, Guangdong, P.R. China

    Sufang Niu

  4. Faculty of Mathematics and Computer Science, College of Software Engineering, Guang Dong Ocean University, Zhanjiang, Guangdong, P.R. China

    Fuguang Yang

  5. Department of Food Science and Engineering, College of Food Science and Technology, Guang Dong Ocean University, Zhanjiang, Guangdong, P.R. China

    Jianping Chen

Authors
  1. Ying Liu
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  2. Guoxuan Liu
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  3. Yali Yang
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  4. Sufang Niu
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  5. Fuguang Yang
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  6. Shaoxia Yang
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  7. Jianian Tang
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  8. Jianping Chen
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Correspondence to Ying Liu.

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Liu, Y., Liu, G., Yang, Y. et al. Establishment of an Efficient Plant Regeneration Culture Protocol and Achievement of Successful Genetic Transformation in Jatropha curcas L.. BIOLOGIA FUTURA 68, 428–442 (2017). https://doi.org/10.1556/018.68.2017.4.8

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Keywords

  • Jatropha curcas
  • Petiole explants
  • Plant regeneration
  • Genetic transformation