Hydrogen Peroxide Induced Oxidative Damage on Mechanical Properties of the Articular Cartilage

Abstract

Articular cartilage has unique mechanical and physicochemical properties which are responsible for its load carrying capabilities. This work investigates the effects of hydrogen peroxide induced oxidative damage on mechanical properties of articular cartilage. Bovine articular cartilage was exposed to hydrogen peroxide for a week. Dynamic and static mechanical tests applied to calculate articular cartilage compressive modulus. We observed higher control curve slopes than that of hydrogen peroxide curves which account for lesser stiffness values in the exposed articular cartilage. For the instantaneous experiments, results were statistically significant (p = 0.01, p > 0.05). Here report that hydrogen peroxide induced oxidative damage causes reduction in the stiffness of the articular cartilage.

References

  1. 1.

    Atiyeh, B. S., Dibo, S. A., Hayek, S. N. (2009) Wound cleansing, topical antiseptics and wound healing. Int. Wound J. 6, 420–430.

    Article  Google Scholar 

  2. 2.

    Bader, D. L., Kempson, G. E., Egan, J., Gilbey, W., Barrett, A. J. (1992) The effects of selective matrix degradation on the short-term compressive properties of adult human articular cartilage. Biochim. Biophys. Acta. 1116, 147–154.

    CAS  Article  Google Scholar 

  3. 3.

    Bhatti, F. U. R., Mehmood, A., Wajid, N., Rauf, M., Khan, S. N., Riazuddin, S. (2013) Vitamin e protects chondrocytes against hydrogen peroxide-induced oxidative stress in vitro. Inflamm. Res. 62, 781–789.

    CAS  Article  Google Scholar 

  4. 4.

    Bonifacio, A., Beleites, C., Vittur, F., Marsich, E., Semeraro, S., Paoletti, S., Sergoa, V. (2010) Chemical imaging of articular cartilage sections with Raman mapping, employing uni- and multivariate methods for data analysis. Analys. 135, 3193–3204.

    CAS  Article  Google Scholar 

  5. 5.

    Brandl, A., Hartmann, A., Bechmann, V., Graf, B., Nerlich, M., Angele, P. (2011) Oxidative stress induces senescence in chondrocytes. J. Orthop. Res. 29, 1114–1120.

    CAS  Article  Google Scholar 

  6. 6.

    Cicek E. (2016) Effect of X-ray irradiation on articular cartilage mechanical properties. Acta Phys. Pol.. 129, 200–202.

    CAS  Article  Google Scholar 

  7. 7.

    Drosou, A., Falabella, A., Kirsner, R. (2003) Antiseptics on wounds: an area of controversy. Wound. 15, 149–166.

    Google Scholar 

  8. 8.

    Greenwald, R. A., Moy, W. W. (1979) Inhibition of collagen gelation by action of the superoxide radical. Arthritis Rheum. 22, 251–259.

    CAS  Article  Google Scholar 

  9. 9.

    Henrotin, Y. E., Bruckner, P., Pujol, J. P. L. (2003) The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthr. Cartil. 11, 747–755.

    CAS  Article  Google Scholar 

  10. 10.

    Huber, M., Trattnig, S., Lintner, F. (2000) Anatomy, Biochemistry, and Physiology of Articular Cartilage. Invest. Radiol. 35, 573–580.

    CAS  Article  Google Scholar 

  11. 11.

    Julkunen, P., Wilson, W., Jurvelin, J. S., Rieppo, J., Qu, C. J., Lammi, M. J., Korhonen, R. K. (2008) Stress-relaxation of human patellar articular cartilage in unconfined compression: Prediction of mechanical response by tissue composition and structure. J. Biomech. 41, 1978–1986.

    Article  Google Scholar 

  12. 12.

    Korhonen, R. K., Laasanen, M. S., Toyras, J., Lappalainen, R., Helminen, H. J., Jurvelin, J. S. (2003) Fibril reinforced poroelastic model predicts specifically mechanical behavior of normal, proteoglycan depleted and collagen degraded articular cartilage. J. Biomech. 36, 1373–1379.

    Article  Google Scholar 

  13. 13.

    Laasanen, M. S., Töyräs, J., Korhonen, R. K., Rieppo, J., Saarakkala, S., Nieminen, M., Hirvonen, J., Jurvelin, J. (2003) Biomechanical properties of knee articular cartilage. Biorheolog. 40, 133–140.

    CAS  Google Scholar 

  14. 14.

    Lean, J., Davies, J., Fuller, K., Jagger, C., Kirstein, B., Partington, G., Urry, Z., Chambers, T. (2003) A crucial role for thiol antioxidants in estrogen-deficiency bone loss. J. Clin. Invest. 112, 915–923.

    CAS  Article  Google Scholar 

  15. 15.

    Monboisse, J. C., Braquet, P., Randoux, A., Borel, J. P. (1983) Non-enzymatic degradation of acidsoluble calf skin collagen by superoxide ion: protective effect of flavonoids. Biochem. Pharmacol. 32, 53–58.

    CAS  Article  Google Scholar 

  16. 16.

    Mow, V. C., Ratcliffe, A., Poole, A. R. (2000) Cartilage and diarthrodial joints as paradigms for hierarchical materials and structures. Biomaterial. 13, 67–97.

    Article  Google Scholar 

  17. 17.

    Na, J. Y., Song, K., Kim, S., Kwon, J. (2016) Rutin protects rat articular chondrocytes against oxidative stress induced by hydrogen peroxide through SIRT1 activation. Biochem. Biophys. Res. Commun. 473, 1301–1308.

    CAS  Google Scholar 

  18. 18.

    Omata, S., Sawae, Y., Murakami, T. (2012) Tissue Development and Mechanical Property in the Regenerated-Cartilage Tissue. In: Ceccherini-Nelli, L., Matteoli, B. (ed.) Biomedical Tissue Culture. InTech, Italy, pp. 1–8.

    Google Scholar 

  19. 19.

    Ouyang, X., Wei, B., Hong, S. D., Wang, J. R., Xin, F., Wang, L., Yang, X. W., Wang, L. M. (2015) Study on the Mechanisms of Cartilage Tissue Damage Caused by Hydrogen Peroxide. Cell Biochem. Biophys. 72, 343–348

    CAS  Article  Google Scholar 

  20. 20.

    Röhner, E., Kolar, P., Seeger, J. B., Arnholdt, J., Thiele, K., Perka, C., Matziolis, G. (2011) Toxicity of antiseptics against chondrocytes: What is best for the cartilage in septic joint surgery? Int. Orthop. 35, 1719–1723.

    Article  Google Scholar 

  21. 21.

    Schaumburger, J., Beckmann, J., Springorum, H., Handel, M., Anders, S., Kalteis, T., Grifka, J., Rath, B. (2010) Toxizität lokaler Antiseptika auf Chondrozyten in vitro. Z. Orthop. Unfall. 148, 39–43.

    CAS  Article  Google Scholar 

  22. 22.

    Young, I. C., Chuang, S. T., Hsu, C. H., Sun, Y. J., Lin, F. H. (2016) C-phycocyanin alleviates osteoarthritic injury in chondrocytes stimulated with H2O2 and compressive stress. Int. J. Biol. Macromol. 93, 852–859.

    CAS  Article  Google Scholar 

  23. 23.

    Zhuang, C., Xu, N. W., Gao, G. M., Ni, S., Miao, K. S., Li, C. K., Wang, L. M., Xie, H. G. (2016) Polysaccharide from Angelica sinensis protects chondrocytes from H2O2-induced apoptosis through its antioxidant effects in vitro. Int. J. Biol. Macromol. 87, 322–328.

    CAS  Article  Google Scholar 

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Correspondence to Ekrem Cicek.

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Cicek, E. Hydrogen Peroxide Induced Oxidative Damage on Mechanical Properties of the Articular Cartilage. BIOLOGIA FUTURA 68, 368–375 (2017). https://doi.org/10.1556/018.68.2017.4.3

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Keywords

  • Antiseptic
  • articular cartilage
  • compressive modulus
  • hydrogen peroxide