Evaluation of Antiproliferative and Hepatoprotective Effects of Wheat Grass (Triticum Aestivum)
This study was aimed to evaluate the pharmacological potential of various extracts (hexane, chloroform, methanol and aqueous) of dried shoots of Triticum aestivum (wheat grass) in terms of antiproliferative and hepatoprotective potential of T. aestivum. The total chlorophyll content in dried shoots of T. aestivum was 0.54 ± 0.016 g/L (chlorophyll-a: 0.288 ± 0.05 g/L; and chlorophyll-b; 0.305 ± 0.05 g/L), while total carotene content was 0.42 ± 0.066 g/L. In addition, the chloroform extract of dried shoots of T. aestivum (250 µg/mL) exhibited 87.23% inhibitory effect with potent cytotoxicity against human hepatocellular carcinoma (HepG2) cancer cell line. Moreover, chloroform and methanol extracts significantly reduced the levels of SGOT, and SGPT enzymes, as well as total bilirubin content, while raised the level of total protein in a concentration-gradient manner, confirming the potent hepatoprotective effect of T. aestivum. A possible mechanism of apoptosis of the chloroform extract of dried shoots of T. aestivum in terms of its potent antiproliferative activity against HepG2 cancer cell line can also be proposed in this study. Our findings clearly demonstrate that T. aestivum has a significant pharmacological potential that night be used for antiproliferative and hepatoprotective purposes.
KeywordsT. aestivum (wheat grass) antiproliferative hepatoprotective apoptosis mechanism pharmacological significance
Unable to display preview. Download preview PDF.
- 2.Adewusi, E. A., Afolayan, A. J. (2010) A review of natural products with hepatoprotective activity. J. Med. Plant Res. 4, 1318–1334.Google Scholar
- 4.Cervantes-Paz, B., Yahia, E. M., Ornelas-Paz, J. J., Victoria-Campos, C. I., Ibarra-Junquera, V, Perez-Martinez, J. D., Escalante-Minakata, P. (2014) Antioxidant activity and content of chlorophylls and carotenoids in raw and heat-processed Jalapeño peppers at intermediate stages of ripening. Food Chem. 146, 188–196.CrossRefGoogle Scholar
- 9.Jayaraman, J. (2011) Estimation of Chlorophyll and Carotenoids. In Laboratory Manual in Biochemistry. New Age. Int. 1, 141–142.Google Scholar
- 10.Lee, R. H., Cho, J. H., Jeon, Y. J., Bang, W., Cho, J. J., Choi, N. J., Seo, K. S., Shim, J. H., Chae, J. I. (2015) Quercetin induces antiproliferative activity against human hepatocellular carcinoma (HepG2) cells by suppressing specificity protein 1 (Sp1). Drug Dev. Res. doi:10.1002/ddr.21235.Google Scholar
- 11.Maekawa, L., Lamping, R., Marcacci, M., Maekawa, M., Nassri, M., Koga, I. C. (2007) Antimicrobial activity of chlorophyll based solutions o. Candida albicans an. E. faecalis. RSBO 4, 36–40.Google Scholar
- 12.Malick, C. P., Singh, M. B. (1980) Plant Enzymology and Histoenzymology. Kalyani Publishers, New Delhi.Google Scholar
- 14.Reddy, V. R., Bikshapathi, G., Reddy, K. M. (2014) Hepatoprotective activity of extract o. Erythroxylum monogynum in albino rats. Int. J. Pure Appl. Biosci. 2, 58–62.Google Scholar
- 16.Shenoy, K. A., Soumyaji, S. N., Bairy, K. L. (2001) Hepatoprotective effect o. Ginkgo Biloba in carbon tetrachloride induced hepatic injury in rats. Indian J. Pharmacol. 33, 260–264.Google Scholar
- 17.Singh, S., Mehta, A., Mehta, P. (2011) Hepatoprotective activity o. Cajanus cajan against carbon tetrachloride induced liver damage. Int. J. Pharm. Sci. 3, 146–147.Google Scholar
- 18.Singh, N., Verma, P., Pandey, B. R. (2012) Therapeutic potential of organi. Triticum aestivum Linn. (wheat grass) in prevention and treatment of chronic diseases: An overview. Int. J. Pharm. Sci. Drug Res. 4, 10–14.Google Scholar
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.