Salinity is one of the major abiotic stress factors affecting series of morphological, physiological, metabolic and molecular changes in plant growth. The effect of different concentrations (0, 25, 50, 100 and 150 mM) of NaCl on the vegetative growth and some physiological parameters of karkade (Hibiscus sabdariffa var. sabdariffa) seedling were investigated. NaCl affected the germination rate, delayed emergence and retarded vegetative growth of seedlings. The length of seedling as well as the leaf area was significantly reduced. The fresh weight remained lower in NaCl treated seedlings compared to control. NaCl at 100 and 150 mM concentrations had significant effect on the dry matter contents of the treated seedlings. The chloroplast pigments in the treated seedlings were affected, suggesting that the NaCl had a significant effect on the chlorophyll and carotenoid biosynthesis. The results showed that the salt treatments induced an increase in proline concentration of the seedlings. The osmotic potential (ψs) of NaCl treated seedlings decreased with increasing NaCl concentrations. Salt treatments resulted in dramatic quantitative reduction in the total sterol percent compared with control ones. Salt stress resulted in increase and decrease of Na+ and K+ ions, respectively. NaCl salinity increased lipid peroxidation. SDSPAGE was used to evaluate protein pattern after applying salt stress. High molecular weight proteins were intensified, while low molecular weight proteins were faint. NaCl at 100 and 150 mM concentration distinguished with new protein bands. Salt stress induced a new peroxidase bands and increased the band intensity, indicating the protective role of peroxidase enzyme.
A.O.A.C. (1986) Official analysis of the association of official analytical chemist. 14th ed. Washington, D.C.
Akinci, I. E., Akinci, S., Yilmaz, K., Dikici, H. (2004) Response of eggplant varieties (Solanum melongena) to salinity in germination and seedling stages. New Zealand J. Crop Horticul. Sci. 32, 193–200.
Ali, H. M., Siddiqui, M. H., Basalah, M. O., Al-Whaibi, M. H., Sakran, A. M., Al-Amri, A. (2012) Effects of gibberellic acid on growth and photosynthetic pigments of Hibiscus sabdariffa L. under salt stress. Afr. J. Biotechnol. 11, 800–804.
Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Inaba, M., Murata, N. (2000) Photosystems I and II in Synechococcus sp. Plant Physiol. 123, 1047–1056.
Almansouri, M., Kine, T. J. M., Lutts, S. (2001) Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant Soil 231, 243–254.
Andriolo, J. L., Luz, G. L., Witter, M. H., Godoi, R. S., Barros, G. T. (2005) Bortolotto OC Growth and yield of lettuce plants under salinity. Horticul. Brasil., Brasil. 23, 931–934.
Azevedo, N. A. D., Tabosa, J. N. (2000) Salt stress in maize seedlings: II. Distribution of cationic macronutrients and it’s relation with sodium. Rev. Bras. Eng. Agric. Amb. 4, 165–171.
Azoo, M. M. (2009) Foliar application of riboflavin (Vitamin B2) enhancing the resistance of Hibiscus sabdariffa L. (deep red petals variety) to salinity stress. J. Biol. Sci. 9, 109–118.
Azooz, M. M., Shaddad, M. A., Abdel-Latef, A. A. (2004) The accumulation and compartmentation of proline in relation to salt tolerance of three sorghum cultivars. Ind. J. Plant Physiol. 9, 1–8.
Bajji, M., Lutts, S., Kient, J. M. (2001) Water deficit effects on solute contribution to osmotic adjustment as a function of leaf aging in three durum wheat (Triticum durum Defs.) cultivars performing differently in arid conditions. Plant Sci. 160, 669–681.
Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39, 205–207.
Bellani, L. M., Guarnieri, M., Scialabba, A. (2002) Differences in the activity and distribution of peroxidases from three different portions of germinating Brassica oleracea seed. Physiol. Plant 114, 102–108.
Binzel, M. L., Reuveni, M. (1994) Cellular mechanisms of salt tolerance in plant cells. Hort. Rev. 16, 33–70.
Bor, M., Ozdemir, F., Turkan, I. (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet (Beta vulgaris L.) and wild beet (Beta maritima L.). Plant Sci. 164, 77–84.
Cha-um, S., Kirdmanee, C. (2009) Effect of salt stress on proline accumulation, photosynthetic ability and growth characters in two maize cultivars. Pak. J. Bot. 41, 87–98.
Cramer, G. R., Lauchli, A., Polito, V. S. (1985) Displacement of Ca from the plasmalemma of root cell. A primary response to salt stress. Plant Physiol. 79, 207–211.
Czabator, F. J. (1962) Germination value: An index combining speed and completeness of pine seed germination. Forest Sci. 8, 386–395.
Dagar, J. C., Bhagwan, H., Kumar, Y. (2004) Effect on growth performance and biochemical contents of Salvadora persica when irrigated with water of different salinity. Ind. J. Plant Physiol. 9, 234–238.
El Naim, A. M., Ahmed, S. E. (2010) Effect of weeding frequencies on growth and yield of two roselle (Hibiscus sabdariffa L.) varieties under rain fed. Aust. j. basic appl. Sci. 4, 4250–4255.
El-Sherif, M. H., Sarwat, M. I. (2007) Physiological and chemical variations in producing roselle plant (Hibiscus sabdariffa L.) by using some organic farmyard manure. World J. Agricul. Sci. 3, 609–616.
Farag, R. S., Hallabo, S. A. S., Hewedi, F. M., Basyony, A. E. (1986) Chemical evaluator of rap seed–Feheseifen. Anstrichmi Hel. 88, 391–397.
Farida, A. K., Das, A. B., Mittra, B., Mohanty, P. (2004) Salt-stress induced alterations in protein profile and protease activity in the mangrove (Bruguiera parvi). Z Naturforsch C. 59, 408–414.
Fernandes, P., Cabral, J. M. S. (2007) Phytosterols: applications and recovery methods. Bioresou. Tech. 9, 2335–2350.
Flowers, T. J., Yeo, A. R. (1989) Effects of salinity on plant growth and crop yield. In: Cherry, J. H. (Ed.). Environmental Stress in Plants. Springer-Verlag: Berlin, pp. 101–119.
Gaspar, T., Penel, C., Castillo, F. J., Greppin, H. (2001) A two-step control of basic and acidic peroxidases and its significance for growth and development. Physiol. Plant 64, 418–423.
Hartmann, M. A. (2004) Sterol metabolism and function in higher plants. In: Daum, G. (Ed.) Lipid Metabolism and Membrane Biogenesis. Springer-Verlag: Heidelberg, pp. 183–211.
Heath, R., Packer, L. (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 196, 385–395.
Hernandez, A., Almansa, M. S. (2002) Short-term effects of salt stress on antioxidant systems and leaf water deficits in leaves. Aust. J. Boil. Sci. 15, 413–428.
Khan, M. H., Panda, S. K. (2008) Alterations in root lipid peroxidation and antioxidative responses in two rice cultivars under NaCl-salinity stress. Acta Physiol. Plant 30, 81–89.
Laemmli, U. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Levitt, J. (1980) Responses of Plants to Environmental Stresses. Vol. II, 2nd ed. Academic Press, New York, 607. p.
Luna, C., Gonzalez, C., Trippi, V. (1994) Oxidative damage caused by an excess of copper in oat leaves. Plant Cell Physiol. 35, 11–15.
Mahadevan, N., Shivali, K. P. (2009) Hibiscus sabdariffa Linn: An overview. Natural Product Radiance 8, 77–83.
Mandhania, S., Madan. S., Sawhney, V. (2006) Antioxidant defense mechanism under salt stress in wheat seedlings. Biol. Plant. 227, 227–231.
Metzner, R. H., Rau, H., Senger, H. (1965) Untersuchunger zur Synchronisierbarkeit einzelner-pigment-Mangel Mutanten Von Chlorella. Planta 65, 186–194.
Muhammad, Z., Hussain, F. (2010) Vegetative growth performance of five medicinal plants under NaCl salt stress. Pak. J. Bot. 42, 303–316.
Munns, R., Husain, S., Rivelli, A. R., James, R. A., Condon, A. G., Lindsay, M. P., Laguda, E. S., Schachtman, D. P., Hare, R. A. (2002) Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant and Soil 247, 93–105.
Murashige, T., Skooge, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473–497.
Reddy, M. P., Vora, A. B. (2002) Changes in pigment composition, hill reaction activity and saccharides relations of pea leaves. Physiol. Plant 115, 251–257.
Rozbeh, F., Adel, F., Ali, A. (2015) Effect of salt stress on physiological and morphological parameters of rapeseed cultivar. J. Sci. Res. Develop. 2, 111–117.
SAS (2003) Proc User’s Manual, Version 9.1., SAS Institute, Cary, N., US.
Savvas, D., Lenz, F. (2000) Effects of NaCl or nutrient-induced salinity on growth, yield, and composition of eggplants grown in rock wool. Scientia Horticulturae 84, 37–47.
Sharma, P. K., Hall, D. O. (1991) Interaction of salt stress and photo inhibition on photosynthesis in barley and sorghum. J. Plant Physiol. 138, 614–619.
Singh, P. K., Shahi, S. K., Singh, A. P. (2015) Effect of salt stress on physico-chemical changes in maize (Zea maize L.) plants in response to salicylic acid. Ind. J. Plant Sci. 4, 69–77.
Stigmann, H., Burgermeister, W., Francksen, H., Krogerrecklen, F. (1983) Manual of gel electrophoresis and isoelectrofocusing with the apparatus Planta–Phor. Inst. Biochem., Messeweg II. D-3300 Braunschweig West Germany.
Taleisnik, E., Peyrano, G., Arias, C. (1997) Response of Chloris gayana cultivars to salinity. 1. Germination and early vegetative growth. Trop. Grassl. 31, 232–240.
Turner, N. C. (1981) Techniques and experimental approaches for the measurement of plant water status. Plant Chem. 28, 350–356.
Wilson, J. R., Ludlow, M. M., Fisher, M. J., Schulze, E. E. (1989) Adaptations to water stress of the leaf water relations of four tropical forage species. Aust. J. Plant Physiol. 7, 207–220.
Zhu, J. K. (2003) Regulation of ion homeostasis under salt stress. Curr. Opin. Plant Biol. 6, 441–445.
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Galal, A. Physico-Chemical Changes in Karkade (Hibiscus sabdariffa L.) Seedlings Responding to Salt Stress. BIOLOGIA FUTURA 68, 73–87 (2017). https://doi.org/10.1556/018.68.2017.1.7
- salt stress
- seedling emergence