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Acta Biologica Hungarica

, Volume 67, Issue 3, pp 318–332 | Cite as

D-Galactose Catabolism in Penicillium Chrysogenum: Expression Analysis of the Structural Genes of the Leloir Pathway

  • Ágota Jónás
  • Erzsébet Fekete
  • Zoltán Németh
  • Michel Flipphi
  • Levente KaraffaEmail author
Article

Abstract

In this study, we analyzed the expression of the structural genes encoding the fve enzymes comprising the Leloir pathway of D-galactose catabolism in the industrial cell factory Penicillium chrysogenum on various carbon sources. The genome of P. chrysogenum contains a putative galactokinase gene at the annotated locus Pc13g10140, the product of which shows strong structural similarity to yeast galactokinase that was expressed on lactose and D-galactose only. The expression profle of the galactose-1-phosphate uridylyl transferase gene at annotated locus Pc15g00140 was essentially similar to that of galactokinase. This is in contrast to the results from other fungi such as Aspergillus nidulans, Trichoderma reesei and A. niger, where the ortholog galactokinase and galactose-1-phosphate uridylyl transferase genes were constitutively expressed. As for the UDP-galactose-4-epimerase encoding gene, fve candidates were identifed. We could not detect Pc16g12790, Pc21g12170 and Pc20g06140 expression on any of the carbon sources tested, while for the other two loci (Pc21g10370 and Pc18g01080) transcripts were clearly observed under all tested conditions. Like the 4-epimerase specifed at locus Pc21g10370, the other two structural Leloir pathway genes–UDP-glucose pyrophosphorylase (Pc21g12790) and phosphoglucomutase (Pc18g01390)–were expressed constitutively at high levels as can be expected from their indispensable function in fungal cell wall formation.

Keywords

Penicillium chrysogenum lactose D-galactose Leloir pathway 

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© Akadémiai Kiadó, Budapest 2016

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Ágota Jónás
    • 1
  • Erzsébet Fekete
    • 1
  • Zoltán Németh
    • 1
  • Michel Flipphi
    • 1
  • Levente Karaffa
    • 1
    Email author
  1. 1.Department of Biochemical Engineering, Faculty of Science and TechnologyUniversity of DebrecenDebrecenHungary

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