Changes in Antioxidant Enzymes Activities and Proline, Total Phenol and Anthocyanine Contents in Hyssopus Officinalis L. Plants Under Salt Stress
The relationships between salt stress and antioxidant enzymes activities, proline, phenol and anthocyanine contents in Hyssopus officinalis L. plants in growth stage were investigated. The plants were subjected to five levels of saline irrigation water, 0.37 (tap water as control) with 2, 4, 6, 8 and 10 dSm–1 of saline water. After two months the uniform plants were harvested for experimental analysis. Antioxidant enzymes activities and proline, phenol and anthocyanine contents of the plants were examinated. Enhanced activities of peroxidase, catalase and superoxide dismutase were determined by increasing salinity that plays an important protective role in the ROS-scavenging process. Proline, phenol and anthocyanine contents increased significantly with increasing salinity. These results suggest that salinity tolerance of Hyssopus officinalis plants might be closely related with the increased capacity of antioxidative system to scavenge reactive oxygen species and with the accumulation of osmoprotectant proline, phenol and anthocyanine contents under salinity conditions.
KeywordsAbiotic stress antioxidant enzymes proline salinity Hyssopus officinalis
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- 2.Ashraf, M. A., Ashraf, M., Ali, Q. (2010) Response of two genetically diverse wheat cultivars to salt stress at different growth stages: leaf lipid peroxidation and phenolic contents. Pak. J. Bot. 42, 559–565.Google Scholar
- 9.Buchanan, B. B., Gruissem, W., Jones, R. (2000) Biochemistry and Molecular Biology of Plants. The American Society of Plant Physiologists. USA. Maryland.Google Scholar
- 16.Fathiazad, F., Hamedeyazdan, S. (2011) A review on Hyssopus officinalis L.: Composition and biological activities. Afr. J. Pharm. Pharmacol. 5, 1959–1966.Google Scholar
- 23.Kizil, S., Toncer, O., Ipek, A., Arslan, N., Saglam S., Khawar, K. M. (2008) Blooming stages of Turkish hyssop (Hyssopus officinalis L.) affect essential oil composition. Acta Agric. Scand, Sect. B. 58, 273–279.Google Scholar
- 33.Sadder, M. T., Anwar, F., Al-Doss, A. A. (2013) Gene expression and physiological analysis of Atriplex halimus (L.) under salt stress. Aust. J. Crop Sci. 7, 112–118.Google Scholar
- 34.Singleton, U. L., Rossi, J. A. (1965) Colorimetry of total phenolics with phosphomolybdic- posphotungustic acid reagent. Am. J. Enol. Vitic. 16, 144–158.Google Scholar
- 36.Tereshchenko, O. Y., Gordeeva, E. I., Arbuzova, V. S., Khlestkina, E. K. (2012) Anthocyanin pigmentation in Triticum aestivum L.: Genetc basis and role under abiotic stress conditions. J. Stress Physiol. Biochem. 8, pp.16.Google Scholar
- 39.Wolski, T., Baj, T., Kwiatkowski, S. (2006) Hyzop lekarski (Hyssopus officinalis L.) zapomniana roślina lecznicza, przyprawowa oraz miododajna. Annales Universitatis Mariae Curie-Skłodowska Lublin-Polonia. 61, 1–10.Google Scholar
- 42.Zhu, H., Chen, X., Pan, X., Zhang, D. (2011) Effects of chloramphenicol on pigmentation, proline accumulation and chlorophyll fluorescence of maize (Zea mays) seedlings. Int. J. Agric. Biol. 13, 677–682.Google Scholar
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