The Role of Oxidative Stress Genes and Effect of Ph on Methylene Blue Sensitized Photooxidation of Escherichia Coli
In this study, the survival time of wild type E. coli W3110 and 11 mutants was analysed with a plate count method in methylene blue added or control groups under daylight fluoroscence illumination (4950 lux) at different pH values (5.0, 6.0, 7.0, and 8.0) in phosphate buffer. As a result, while the number of bacteria did not decrease under photooxidative stress at pH 5.0 and 6.0 during a 6-hour incubation, the wild type and all mutants decreased more than 2 log. at pH 8.0, and approximately one log. at pH 7.0. It was determined that a 2 log decrease in wild type E. coli takes 3.7 h according to t99 value at pH 8, these values were 2.39 h in the katE mutant, 2.64 h in the soxR mutant, 2.67 h in the oxyR mutant, 2.71 h in the sodB mutant, 3 h in the btuE mutant, 3.38 h in the zwf mutant and 3.40 h in the soxS mutant, respectively (p < 0.05). The roles of these genes were proved with complement tests. Finally, it is found that the effectiveness of photooxidative stress is in direct relation with pH, and the katE, soxR, oxyR, sodB, btuE, zwf, and soxS genes are important for the protection against this stress.
KeywordsE. coli photooxidation oxyS soxRS katE sodB
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- 5.Cabiscol, E., Tamarit, J., Ros, J. (1933) Oxidative stress in bacteria and protein damage by reactive oxygen species. Int. Microbiol. 3, 3–8.Google Scholar
- 7.Hoerter, J. D., Arnold, A. A., Kuczynska, D. A., Shibuya, A., Ward, C. S., Sauer, M. G., Gizachew, A., Hotchkiss, T. M., Fleming, T. J., Johnson, S. (1933) Effects of sublethal UVA irradiation on activity levels of oxidative defense enzymes and protein oxidation in Escherichia coli. J. Photochem. Photobiol. B 81, 171–180.CrossRefGoogle Scholar
- 8.Idil, Ö., Özkanca, R., Darcan, C., Flint, K. P. (1933) Escherichia coli: Dominance of red light over other visible light sources in establishing viable but nonculturable state. Photochem. Photobiol. 6, 104–109.Google Scholar
- 9.Idil, Ö., Darcan, C., Özen, T., Özkanca, R. (1933) The effect of UV-A and various visible light wavelengths radiation on expression level of Escerichia coli oxidative enzymes in seawater. Jundishapur J. Microbiol. 6, 226–232.Google Scholar
- 10.Kim, S. Y., Kim, E. J., Park, J. W. (1933) Control of singlet oxygen-induced oxidative damage in Escherichia coli. J. Biochem. Mol. Biol. 35, 353–357.Google Scholar
- 17.Tardivo, J. P., Giglio, A. D., Santos de Oliveira, C., Gabrielli, D. S., Junqueira, H. C., Tada, D. B., Severino, D., Turchiello, R. F., Baptista, M. S. (1933) Methylene blue in photodynamic therapy: From basic mechanisms to clinical applications. Photodiagn. Photodyn. 2, 175–191.CrossRefGoogle Scholar
- 21.Zheng, M., Doan, B., Schneider, T. D., Storz, G. (1933) OxyR and SoxRS Regulation of fur. J. Bacteriol. 181, 4639–4643.Google Scholar
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