Morphological and Physiological Changes in Esterase and Lipid Peroxidation of Two Bean Cultivars Pre-Soaked With Potassium Nitrate Under Salt Stress


Two broad bean cultivars (Vicia faba CV Nobaria3 and Vicia faba CV Sakha3) were obtained from Mallwi Agriculture Research Center, El Minia Governorate, Egypt. The seeds were divided into two groups, the first group soaked with distilled water, while the second group were soaked with 3 niM KN03, respectively, for 4 hours. Seeds were sown and left to grow for 3 weeks then treated with different concentrations of NaCl (0.0, 40, 80, 120 and 160 mM) by top irrigation, then they left to grow further for 65 days from sowing. Plant samples were collected for some measurements: leaf area, plant height, root length, fresh and dry weight, photo synthetic pigments, carotenoids, soluble sugars, soluble proteins, total free amino acids, esterase enzyme, as well as MDA (malondialdehyde) content. Salinity reduced both fresh and dry weight in two broad bean cultivars, this reduction were more pronounced in Sakha3 than Nobaria3. Seed pre-soaking with KN03 resulted in enhancement of fresh and dry weight production in both cultivars especially at 40 mM NaCl. Photosynthetic pigments were substantially affected by salt treatment while the carotenoids were increased, seed pre-soaking with KN03 improved these components. The soluble sugars, amino acids as well as soluble proteins showed various responses with increasing salinity in the cultivars, seed pre-soaking with KN03 has improved these parameters to some extent. The shoots of two cultivars exhibited significant accumulation of MDA, compared to roots exposed to the highest salinity levels. Pre-soaking seeds with KN03 did not improve MDA in shoots but enhanced it in roots, however, in most cases still lower than the absolute control. The assessment of the esterase isozyme profiles on 7.5% native polyacrylamide gel revealed the presence of 13 isoforms in two faba bean plants in response to KNO3 pre-soaking and treatments with different concentrations of NaCl.









(ethylene diaminetertaacetic acid)




(leaf area)




(trichloroacetic acid)


(water content)


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Correspondence to Gaber K. Abd El-Baki.

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Shaddad, M.A.K., Abd El-Baki, G.K., Doaa, M. et al. Morphological and Physiological Changes in Esterase and Lipid Peroxidation of Two Bean Cultivars Pre-Soaked With Potassium Nitrate Under Salt Stress. BIOLOGIA FUTURA 66, 419–435 (2015).

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  • Amino acids
  • chlorophylls
  • esterase enzyme
  • lipid peroxidation
  • salinity
  • Vicia faba