In Vitro Biological Activity of Secondary Metabolites from Seseli rigidum Waldst. Et Kit. (Apiaceae)
The antioxidant, antimicrobial activity, total phenolic content and flavonoid concentration of Seseli rigidum Waldst. et Kit. were evaluated. Five different extracts of the aboveground plant parts were obtained by extraction with distilled water, methanol, acetone, ethyl acetate and petroleum ether. Total phenols were determined using the Folin-Ciocalteu’s reagent, with the highest values obtained in the acetone extract (102.13 mg GAE/g). The concentration of flavonoids, determined by using a spectrophotometric method with aluminum chloride and expressed in terms of rutin equivalent, was also highest in the acetone extracts (291.58 mg RUE/g). The antioxidant activity was determined in vitro using DPPH reagent. The greatest antioxidant activity was expressed in the aqueous extract (46.15 μg/ml). In vitro antimicrobial activities were determined using a microdilution analysis method; minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. Methanolic extract had the greatest influence on bacilli (MIC at 0.0391 mg/ml), but the best antimicrobial effect had acetone and ethyl acetate extracts considering their broad impact on bacteria. According to our research, S. rigidum can be regarded as promising candidate for natural plant source with high value of biological compounds.
KeywordsAntioxidants antimicrobial activity flavonoids phenols Seseli rigidum
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- 2.Ball, P. W. (1968) Seseli In: Tutin, T. G., Heywood, V. H., Burges, N. A., Moore, D. M., Valentine, D. H., Walters, S. M., Webb, D. A., Flora Europaea II, Cambridge University Press, Cambridge, pp. 334–338.Google Scholar
- 6.El-Agbar, Z., Shakya, A., Khalaf N., Al-Haroon, M. (2008) Comparative antioxidant activity of some edible plants. Turk. J. Biol. 32, 193–196.Google Scholar
- 7.Jakovljević, D., Stanković, M. S., Topuzović, M. (2013) Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. EXCLIJ. 12, 260–268.Google Scholar
- 8.Khan, S., Ullah, F., Mahmood, T. (2013) In vitro antimicrobial and cytotoxic activity of Tamarix dioica Roxb. leaves. Turk. J. Biol. 37, 329–335.Google Scholar
- 9.Koksal, E., Bursal, E., Dikici, E., Tozoglu, F., Gulcin, I. (2011) Antioxidant activity of Melissa officinalis leaves. J. Med. Plant. Res. 5, 217–222.Google Scholar
- 16.Matejić, J. S., Džamić, A. M., Mihajilov-Krstev, T., Ranđelović, V. N., Krivošej, Z. Đ., Marin, P. D. (2012) Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Centr Eur. J. Biol. 7, 1116–1122.Google Scholar
- 18.Nikolić, V. (1973) Seseli. In: Josifović, M. (ed.) Flora of Republic of Serbia. SANU, Belgrade, pp. 241–249.Google Scholar
- 21.Šiljegović, J., Glamočlija, J., Soković, M., Vučković, I., Tešević, V., Milosavljević, S., Stešević, D. (2011) Composition and antimicrobial activity of Seseli montanum subsp. tommasinii essential oil. N.P.C. 6, 263–266.Google Scholar
- 22.Stanković, M. S., Nićiforović, N., Topuzović, M., Solujić, S. (2011) Total phenolic content, flavonoid concentrations and antioxidant activity of the whole plant and plant parts extracts from Teucrium montanum L. var. montanum, f supinum (L.) Reichenb. Biotechnol. Biotec. Eq. 25, 2222–2227.CrossRefGoogle Scholar
- 26.Turkoglu, S., Turkoglu, I., Kahyaoglu M., Cehk, S. (2010) Determination of antimicrobial and antioxidant activities of Turkish endemic Ajuga chamaepitys (L.) Schreber subsp. euphratica PH. Davis (Lamiaceae). J. Med. Plant. Res. 4, 1260–1268.Google Scholar
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