Advertisement

Acta Biologica Hungarica

, Volume 66, Issue 4, pp 395–405 | Cite as

In Vitro Biological Activity of Secondary Metabolites from Seseli rigidum Waldst. Et Kit. (Apiaceae)

  • Dragana JakovljevićEmail author
  • Sava Vasić
  • Milan Stanković
  • Ljiljana Čomić
  • Marina Topuzović
Article

Abstract

The antioxidant, antimicrobial activity, total phenolic content and flavonoid concentration of Seseli rigidum Waldst. et Kit. were evaluated. Five different extracts of the aboveground plant parts were obtained by extraction with distilled water, methanol, acetone, ethyl acetate and petroleum ether. Total phenols were determined using the Folin-Ciocalteu’s reagent, with the highest values obtained in the acetone extract (102.13 mg GAE/g). The concentration of flavonoids, determined by using a spectrophotometric method with aluminum chloride and expressed in terms of rutin equivalent, was also highest in the acetone extracts (291.58 mg RUE/g). The antioxidant activity was determined in vitro using DPPH reagent. The greatest antioxidant activity was expressed in the aqueous extract (46.15 μg/ml). In vitro antimicrobial activities were determined using a microdilution analysis method; minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined. Methanolic extract had the greatest influence on bacilli (MIC at 0.0391 mg/ml), but the best antimicrobial effect had acetone and ethyl acetate extracts considering their broad impact on bacteria. According to our research, S. rigidum can be regarded as promising candidate for natural plant source with high value of biological compounds.

Keywords

Antioxidants antimicrobial activity flavonoids phenols Seseli rigidum 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Andrews, J. M. (2005) BSAC standardized disc susceptibility testing method (version 4). J. Antimicrob. Chemother. 56, 60–76.CrossRefGoogle Scholar
  2. 2.
    Ball, P. W. (1968) Seseli In: Tutin, T. G., Heywood, V. H., Burges, N. A., Moore, D. M., Valentine, D. H., Walters, S. M., Webb, D. A., Flora Europaea II, Cambridge University Press, Cambridge, pp. 334–338.Google Scholar
  3. 3.
    Bulatović, V. M., Šavikin-Fodulović, K. P., Zdunić, G. M., Popović, M. P. (2006) Essential oil of Seseli peucedanoides (MB) Kos.-Pol. J. Essent. Oil Res. 18, 286–287.CrossRefGoogle Scholar
  4. 4.
    Chebil, L., Humeau, C., Anthoni, J., Dehez, E., Engasser, J., Ghoul, M. (2007) Solubility of flavonoids in organic solvents. J. Chem. Eng. Data 52, 1552–1556.CrossRefGoogle Scholar
  5. 5.
    Ćurčić, M., Stanković, M. S., Radojević, I., Stefanović, O., Čomić, Lj., Topuzović, M., Djačić D., Marković, S. (2012) Biological effects, total phenolic content and flavonoid concentrations of fragrant yellow onion (Alliumflavum L.). Med. Chem. 8, 46–51.CrossRefGoogle Scholar
  6. 6.
    El-Agbar, Z., Shakya, A., Khalaf N., Al-Haroon, M. (2008) Comparative antioxidant activity of some edible plants. Turk. J. Biol. 32, 193–196.Google Scholar
  7. 7.
    Jakovljević, D., Stanković, M. S., Topuzović, M. (2013) Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. EXCLIJ. 12, 260–268.Google Scholar
  8. 8.
    Khan, S., Ullah, F., Mahmood, T. (2013) In vitro antimicrobial and cytotoxic activity of Tamarix dioica Roxb. leaves. Turk. J. Biol. 37, 329–335.Google Scholar
  9. 9.
    Koksal, E., Bursal, E., Dikici, E., Tozoglu, F., Gulcin, I. (2011) Antioxidant activity of Melissa officinalis leaves. J. Med. Plant. Res. 5, 217–222.Google Scholar
  10. 10.
    Kukula-Koch, W., Aligiannis, N., Halabalaki, M., Skaltsounis, A. L., Glowniak, K., Kalpoutzakis, E. (2013) Influence of extraction procedures on phenolic content and antioxidant activity of Cretan barberry herb. Food Chem. 138, 406–413.CrossRefGoogle Scholar
  11. 11.
    Kumarasamy, Y., Byres, M., Cox, P. J., Jasapars, M., Nahar, L., Sarker, S. D. (2007) Screening seeds of some Scottish plants for free-radical scavenging activity. Phytother Res. 21, 615–621.CrossRefGoogle Scholar
  12. 12.
    Küpeli, E., Tosun, A., Yesilada, E. (2006) Anti-inflammatory and antinociceptive activities of Seseli L. species (Apiaceae) growing in Turkey. J. Ethnopharmacol. 104, 310–314.CrossRefGoogle Scholar
  13. 13.
    Lee, S. E., Hwang, H. J., Ha, J. S., Jeong, H. S., Kim, J. H. (2003) Screening of medicinal plant extracts for antioxidant activity. Life Sci. 73, 167–179.CrossRefGoogle Scholar
  14. 14.
    Marčetić, M. D., Lakušić, B. S., Lakušić, D. V., Kovačević, N. N. (2013) Variability of the root essential oils of Seseli rigidum Waldst. et Kit. (Apiaceae) from different populations in Serbia. Chem. Biodivers. 10, 1653–1666.CrossRefGoogle Scholar
  15. 15.
    Marčetić, M., Božić, D., Milenković, M., Lakušić, B., Kovačević, N. (2012) Chemical composition and antimicrobial activity of essential oil of different parts of Seseli rigidum. Nat. Prod. Commun. 7, 1091–1094.PubMedGoogle Scholar
  16. 16.
    Matejić, J. S., Džamić, A. M., Mihajilov-Krstev, T., Ranđelović, V. N., Krivošej, Z. Đ., Marin, P. D. (2012) Total phenolic content, flavonoid concentration, antioxidant and antimicrobial activity of methanol extracts from three Seseli L. taxa. Centr Eur. J. Biol. 7, 1116–1122.Google Scholar
  17. 17.
    Milosavljević, S., Tešević, V., Vučković, I., Jadranin, M., Vajs, V., Soković, M., Janačković, P., Jovanović, A. (2007) Composition and antifungal activity of the essential oil of Seseli annuum wild-growing in Serbia. Fitoterapia 78, 319–322.CrossRefGoogle Scholar
  18. 18.
    Nikolić, V. (1973) Seseli. In: Josifović, M. (ed.) Flora of Republic of Serbia. SANU, Belgrade, pp. 241–249.Google Scholar
  19. 19.
    Ozturk, S., Ercisli, S. (2006) Chemical composition and in vitro antibacterial activity of Seseli libano-tis. World J. Microb. Biot. 22, 261–265.CrossRefGoogle Scholar
  20. 20.
    Sarker, S. D., Nahar, L., Kumarasamy, Y. (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42, 321–324.CrossRefGoogle Scholar
  21. 21.
    Šiljegović, J., Glamočlija, J., Soković, M., Vučković, I., Tešević, V., Milosavljević, S., Stešević, D. (2011) Composition and antimicrobial activity of Seseli montanum subsp. tommasinii essential oil. N.P.C. 6, 263–266.Google Scholar
  22. 22.
    Stanković, M. S., Nićiforović, N., Topuzović, M., Solujić, S. (2011) Total phenolic content, flavonoid concentrations and antioxidant activity of the whole plant and plant parts extracts from Teucrium montanum L. var. montanum, f supinum (L.) Reichenb. Biotechnol. Biotec. Eq. 25, 2222–2227.CrossRefGoogle Scholar
  23. 23.
    Stojković, S., Petrović, S., Kukić, J., Džamić, A., Ristić, M., Milenković, M., Glamočlija, J., Soković, M., Stojković, D. (2009) Chemical composition and antimicrobial and antioxidant activity of Seseli rigidum flower essential oil. Chem. Nat. Comp. 45, 253–256.CrossRefGoogle Scholar
  24. 24.
    Sultana, B., Anwar, F., Ashraf M. (2009) Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14, 2167–2180.CrossRefGoogle Scholar
  25. 25.
    Tosun, A., Kürkçüoglu, M., Dogan, E., Duman, H., Başer, K. H. C. (2006) Essential oil composition of Seseli petraeum M. Bieb. and Seseli andronakii Woron. growing in Turkey. Flavour Frag. J. 21, 257–259.CrossRefGoogle Scholar
  26. 26.
    Turkoglu, S., Turkoglu, I., Kahyaoglu M., Cehk, S. (2010) Determination of antimicrobial and antioxidant activities of Turkish endemic Ajuga chamaepitys (L.) Schreber subsp. euphratica PH. Davis (Lamiaceae). J. Med. Plant. Res. 4, 1260–1268.Google Scholar
  27. 27.
    Wink, M. (2012) Medicinal plants: a source of anti-parasitic secondary metabolites. Molecules 17, 12771–12791.CrossRefGoogle Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Dragana Jakovljević
    • 1
    Email author
  • Sava Vasić
    • 1
  • Milan Stanković
    • 1
  • Ljiljana Čomić
    • 1
  • Marina Topuzović
    • 1
  1. 1.Department of Biology and Ecology, Faculty of ScienceUniversity of KragujevacKragujevacRepublic of Serbia

Personalised recommendations