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Acta Biologica Hungarica

, Volume 66, Issue 3, pp 258–269 | Cite as

Antioxidative Effects of α-Lipoic Acid in the Brain, Liver and Kidneys in selected Mouse Organs Exposed to Zymosan

  • Zofia GocEmail author
  • Agnieszka Greń
  • Edyta Kapusta
  • Karol Dziubek
  • Waldemar Szaroma
Article

Abstract

This study investigated the role of exogenous α-lipoic acid (ALA) in the inflammation caused by zymosan application. Seventy-two adult male white mice were divided into twelve groups: three control groups, three Zymosan groups, three ALA groups and three groups being the combination of Zymosan and ALA. In the experimental groups, the animals were decapitated after 3, 6 and 24 hours after the injection. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined in the brain, liver and kidneys of the mice. After the injection of Zymosan, it was found that the activity of SOD, CAT and GSH-Px in the brain, liver and kidneys of mice was significantly lower in all time periods. The administration of ALA resulted in an opposite effect, namely, it increased the activity of the enzymes studied in the selected organs of mice. The Zymosan and ALA combination significantly inhibited the decrease in the activity of the enzymes compared with the values obtained in the groups of animals which received Zymosan only. The results of our study, using the Zymosan-induced inflammation, clearly indicate that ALA is an anti-inflammatory agent.

Keywords

Superoxide dismutase glutathione peroxidase catalase a-lipoic acid Zymosan 

Abbreviations

ALA

a-lipoic acid

ANOVA

analysis of variance

BSA

bovine serum albumin

b.w.

body weight

CAT

catalase

DHLA

dihydrolipoic acid

EDTA

ethylenediaminetetra acetic acid disodium salt

GSH

reduced glutathione

GSH-Px

glutathione peroxidase

H2O2

hydrogen peroxide

HO

hydroxyl radical

HOCl

hypochlorous acid

i.p.

intraperitoneal injection

K2HPO4

dipotassium hydrogen phosphate

KH2PO4

potassium dihydrogen phosphate

LA

DL-a-lipoic acid

NO

nitric oxide

ONOO

peroxynitrite

PUFA

polyunsaturated fatty acids

RNS

reactive nitrogen species

ROS

reactive oxygen species

1O2

singled oxygen

SOD

superoxide dis-mutase

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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Zofia Goc
    • 1
    Email author
  • Agnieszka Greń
    • 1
  • Edyta Kapusta
    • 1
  • Karol Dziubek
    • 1
  • Waldemar Szaroma
    • 1
  1. 1.Department of Animal Physiology and Toxicology, Institute of BiologyPedagogical University of CracowCracowPoland

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