Acta Biologica Hungarica

, Volume 66, Issue 2, pp 222–230 | Cite as

Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus

  • Yoon J. Kim
  • Mi S. Lim
  • Su M. Kim
  • Ki H. Ryu
  • Sun H. ChoiEmail author


Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.


Tobacco rattle virus Gladiolus Korean isolate RNA2 phylogeny 


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© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Yoon J. Kim
    • 1
  • Mi S. Lim
    • 1
  • Su M. Kim
    • 1
  • Ki H. Ryu
    • 1
  • Sun H. Choi
    • 1
    Email author
  1. 1.Department of Horticulture, Biotechnology and Landscape ArchitectureSeoul Women’s UniversitySeoulKorea

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