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Acta Biologica Hungarica

, Volume 66, Issue 2, pp 222–230 | Cite as

Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus

  • Yoon J. Kim
  • Mi S. Lim
  • Su M. Kim
  • Ki H. Ryu
  • Sun H. ChoiEmail author
Article

Abstract

Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.

Keywords

Tobacco rattle virus Gladiolus Korean isolate RNA2 phylogeny 

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Copyright information

© Akadémiai Kiadó, Budapest 2015

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Yoon J. Kim
    • 1
  • Mi S. Lim
    • 1
  • Su M. Kim
    • 1
  • Ki H. Ryu
    • 1
  • Sun H. Choi
    • 1
    Email author
  1. 1.Department of Horticulture, Biotechnology and Landscape ArchitectureSeoul Women’s UniversitySeoulKorea

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