Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus

Abstract

Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.

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Correspondence to Sun H. Choi.

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Kim, Y.J., Lim, M.S., Kim, S.M. et al. Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus. BIOLOGIA FUTURA 66, 222–230 (2015). https://doi.org/10.1556/018.66.2015.2.8

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Keywords

  • Tobacco rattle virus
  • Gladiolus
  • Korean isolate
  • RNA2
  • phylogeny