Molecular Characterization of the Tobacco Rattle Virus RNA2 Genome Isolated from Gladiolus
Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
KeywordsTobacco rattle virus Gladiolus Korean isolate RNA2 phylogeny
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- 6.Koenig, R., Lesemann, D. E., Pfeilstetter, E., Winter, S., Pleij, C. W. A. (2011) Deletions and recombinations with the RNA1 3’ ends of different tobraviruses have created a multitude of tobacco rattle virus TCM-related RNA2 species in Alstroemeria and tulip. J. Gen. Virol. 92, 988–996.CrossRefGoogle Scholar
- 9.Park, I. S., Chang, M. U., Kim, K. W., Kyun, H. J. (1998) The viruses in Gladiolus hybridus cultivated in Korea 2. Broad bean wilt virus, Cucumber mosaic virus and Tobacco rattle virus. Plant Pathol. J. 14, 83–91.Google Scholar
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