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International Journal of Hematology

, Volume 84, Issue 2, pp 143–150 | Cite as

Interferon γ Has Dual Potential in Inhibiting or Promoting Survival and Growth of Hematopoietic Progenitors: Interactions with Stromal Cell-Derived Factor 1

  • Jin-Hee Hwang
  • Seong-Woo Kim
  • Hyo-Jin Lee
  • Hwan-Jung Yun
  • Samyong Kim
  • Deog-Yeon Jo
Review Article

Abstract

We explored the possibility that interferon γ (IFN-γ) has bidirectional functions in the survival and growth of hematopoietic progenitors, especially with regard to interactions with stromal cell-derived factor 1 (SDF-1). IFN-γ partially rescued normal bone marrow CD34+ cells and colony-forming cells from apoptosis induced by serum and hematopoietic growth factor (HGF) deprivation, and SDF-1 further enhanced cell survival. Short-term IFN-γ treatment of CD34+ cells in the absence of serum and HGFs enhanced the clonal growth of the cells in synergy with SDF-1. In contrast, IFN-γ inhibited the clonal growth of hematopoietic progenitor cells in a standard methylcellulose clonogenic assay and inhibited the HGF-mediated survival of normal CD34+ cells. The addition of SDF-1 did not alter these outcomes. IFN-γ did not enhance SDF-1-induced activation of PI3K/Akt or up-regulate the expression of CXCR4 or its function in bone marrow CD34+ cells. IFN-γ up-regulated Socs1 messenger RNA expression in normal CD34+ cells, which was further enhanced with the addition of HGFs. These results indicate that IFN-γ, partly in concert with SDF-1, exerts dual effects on the survival and growth of hematopoietic progenitor cells; the effects of IFN-γ on hematopoietic progenitor cells can differ, depending on the particular in vitro experimental conditions, especially the presence of HGFs.

Key words

Interferon-γ Hematopoietic progenitor cells Survival Dual potential SDF-1 

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Copyright information

© The Japanese Society of Hematology 2006

Authors and Affiliations

  • Jin-Hee Hwang
    • 1
  • Seong-Woo Kim
    • 1
  • Hyo-Jin Lee
    • 1
  • Hwan-Jung Yun
    • 1
  • Samyong Kim
    • 1
  • Deog-Yeon Jo
    • 1
  1. 1.Division of Hematology/Oncology, Department of Internal MedicineChungnam National University HospitalDaejonKorea

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