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Interferon γ Has Dual Potential in Inhibiting or Promoting Survival and Growth of Hematopoietic Progenitors: Interactions with Stromal Cell-Derived Factor 1

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Abstract

We explored the possibility that interferon γ (IFN-γ) has bidirectional functions in the survival and growth of hematopoietic progenitors, especially with regard to interactions with stromal cell-derived factor 1 (SDF-1). IFN-γ partially rescued normal bone marrow CD34+ cells and colony-forming cells from apoptosis induced by serum and hematopoietic growth factor (HGF) deprivation, and SDF-1 further enhanced cell survival. Short-term IFN-γ treatment of CD34+ cells in the absence of serum and HGFs enhanced the clonal growth of the cells in synergy with SDF-1. In contrast, IFN-γ inhibited the clonal growth of hematopoietic progenitor cells in a standard methylcellulose clonogenic assay and inhibited the HGF-mediated survival of normal CD34+ cells. The addition of SDF-1 did not alter these outcomes. IFN-γ did not enhance SDF-1-induced activation of PI3K/Akt or up-regulate the expression of CXCR4 or its function in bone marrow CD34+ cells. IFN-γ up-regulated Socs1 messenger RNA expression in normal CD34+ cells, which was further enhanced with the addition of HGFs. These results indicate that IFN-γ, partly in concert with SDF-1, exerts dual effects on the survival and growth of hematopoietic progenitor cells; the effects of IFN-γ on hematopoietic progenitor cells can differ, depending on the particular in vitro experimental conditions, especially the presence of HGFs.

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Authors and Affiliations

  1. Division of Hematology/Oncology, Department of Internal Medicine, Chungnam National University Hospital, 640 Daesa-dong, Jung-gu, 301-721, Daejon, Korea

    Jin-Hee Hwang, Seong-Woo Kim, Hyo-Jin Lee, Hwan-Jung Yun, Samyong Kim & Deog-Yeon Jo

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  1. Jin-Hee Hwang
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  2. Seong-Woo Kim
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  3. Hyo-Jin Lee
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Correspondence to Deog-Yeon Jo.

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Hwang, JH., Kim, SW., Lee, HJ. et al. Interferon γ Has Dual Potential in Inhibiting or Promoting Survival and Growth of Hematopoietic Progenitors: Interactions with Stromal Cell-Derived Factor 1. Int J Hematol 84, 143–150 (2006). https://doi.org/10.1532/IJH97.A30606

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  • DOI: https://doi.org/10.1532/IJH97.A30606

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