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International Journal of Hematology

, Volume 83, Issue 1, pp 66–73 | Cite as

Adoptive Transfer of Epstein-Barr Virus-Specific Cytotoxic T-Lymphocytes for the Treatment of Angiocentric Lymphomas

  • Eun-Kyung Kim
  • Sung-Hee Yoon
  • Hyun-Il Cho
  • Tai-Gyu Kim
  • Young Seon Hong
  • Myung Ah Lee
  • Chun-Choo Kim
Article

Abstract

Angiocentric lymphoma, known as natural killer (NK)/T-cell non-Hodgkin’s lymphoma, has been reported to be associated with the Epstein-Barr virus (EBV). We performed adoptive transfer of EBV-specific polyclonal T-cell lines in 3 patients with extranodal NK/T-cell lymphoma, nasal type, and evaluated the treatment for safety, immunologic reconstitution, and clinical outcomes. The tissue samples collected from the 3 patients were confirmed by polymerase chain reaction analysis to be EBV positive. In the cases of the first and second patients, EBV-transformed B-lymphoblastoid cell lines (LCLs) and T-cell lines were generated from peripheral lymphocytes of HLA-matched sibling donors. The third patient’s T-cell lines were induced with autologous lymphocytes. Polyclonal T-cell infusion was carried out after high-dose radiotherapy because active relapsed disease remained in all of the patients. The first patient received 4 weekly infusions of 2 X 107 cells/m2, and the second and third patients underwent treatment with 2 cycles of infusions of the same dosage. All T-cell lines showed >60% NK activity, cytotoxic T-lymphocyte (CTL) responses of >40% against autologous LCLs, and no CTL activity against patient-derived lym-phoblasts. The level of cytotoxicity increased substantially in all patients after cell infusion. The 2 patients who received T-cell therapy twice had stabilized disease for more than 3 years. These safe treatments exhibited no severe inflammatory response, and no serious toxicity developed during T-cell therapy. Our findings demonstrate that adoptively transferred cells may provide reconstitution of EBV-specific CTL responses in patients with active relapsed angiocentric lymphoma. These results provide a rationale for the immunotherapy of angiocentric lymphoma.

Key words

Angiocentric lymphomas Epstein-Barr virus Cytotoxic T-lymphocyte Adoptive transfer Cancer immunotherapy 

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Copyright information

© The Japanese Society of Hematology 2006

Authors and Affiliations

  • Eun-Kyung Kim
    • 1
  • Sung-Hee Yoon
    • 1
  • Hyun-Il Cho
    • 1
    • 2
  • Tai-Gyu Kim
    • 1
    • 2
  • Young Seon Hong
    • 3
  • Myung Ah Lee
    • 3
  • Chun-Choo Kim
    • 1
    • 3
  1. 1.Department of Microbiology and Immunology, Catholic Research Institute of Medical Science, College of MedicineThe Catholic University of KoreaSeocho-Ku, SeoulKorea
  2. 2.Hemopoietic Stem Cell BankThe Catholic University of KoreaSeocho-Ku, SeoulKorea
  3. 3.Division of Oncology, Department of Internal Medicine, Clinic Institute of St. Mary’s Hospital, College of MedicineThe Catholic University of KoreaSeoulKorea

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