Identification of a Chromosomal Breakpoint and Detection of a Novel Form of an MLL- AF17 Fusion Transcript in Acute Monocytic Leukemia with t(11;17)(q23;q21)

Abstract

More than 40 genes have been reported as translocation partners of the mixed lineage leukemia gene(MLL) in hematologic malignancies.AF17 was identified earlier than most otherMLL translocation partners. On the other hand, there is only 1 report of anMLL- AF17 fusion transcript in acute myeloid leukemia (AML). Here we describe a 40-year-old man with a diagnosis of AML involving t(11;17)(q23;q21). We identified a chromosomal breakpoint for t(11;17)(q23;q21) atMLL intron 6 andAF17 intron 8. Although the previously reported form of theMLL- AF17 fusion transcript was not detected by reverse transcriptase-polymerase chain reaction (PCR) analysis, a novel form of anMLL- AF17 fusion transcript joiningMLL exon 6 toAF17 exon 9 was detected by complementary DNA panhandle PCR. The fact that 2 forms ofMLL- AF17 retain the leucine zipper domain ofAF17 suggests that the dimerization domain ofAF17 is critical for leukemogenesis by theMLL- A F17 fusion gene.

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Correspondence to Kazumi Suzukawa or Seiichi Shimizu or Noriko Nemoto or Naoko Takei or Tomohiko Taki or Toshiro Nagasawa.

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Suzukawa, K., Shimizu, S., Nemoto, N. et al. Identification of a Chromosomal Breakpoint and Detection of a Novel Form of an MLL- AF17 Fusion Transcript in Acute Monocytic Leukemia with t(11;17)(q23;q21). Int J Hematol 82, 38–41 (2005). https://doi.org/10.1532/IJH97.05025

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Key words

  • MLL
  • AF17
  • t(11,17)
  • Chromosomal breakpoint
  • Acute monocytic leukemia