Novel Method for Efficient Production of Multipotential Hematopoietic Progenitors from Human Embryonic Stem Cells

  • Feng Ma
  • Dan Wang
  • Sachiyo Hanada
  • Yasuhiro Ebihara
  • Hirohide Kawasaki
  • Yuji Zaike
  • Toshio Heike
  • Tatsutoshi Nakahata
  • Kohichiro Tsuji
Article

Abstract

We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitors in coculture, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial cell potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type β-globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. These progenitors showed neither the immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. The coculture system developed for hESC can provide a novel source of hematopoietic and blood cells for applications in cellular therapy and drug screening.

Key words

Hematopoietic progenitors Human embryonic stem cells Fetal liver Stromal cells 

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Copyright information

© The Japanese Society of Hematology 2007

Authors and Affiliations

  • Feng Ma
    • 1
  • Dan Wang
    • 1
  • Sachiyo Hanada
    • 1
  • Yasuhiro Ebihara
    • 1
  • Hirohide Kawasaki
    • 1
  • Yuji Zaike
    • 2
  • Toshio Heike
    • 3
  • Tatsutoshi Nakahata
    • 3
  • Kohichiro Tsuji
    • 1
  1. 1.Division of Cellular Therapy, Advanced Clinical Research Center, Institute of Medical ScienceUniversity of TokyoTokyoJapan
  2. 2.Department of Laboratory Medicine, Research Hospital, Institute of Medical ScienceUniversity of TokyoTokyoJapan
  3. 3.Department of Pediatrics, Graduate School of MedicineKyoto UniversityKyotoJapan

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