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Production and characterization of surfactant-stable fungal keratinase from Gibberella intermedia CA3-1 with application potential in detergent industry

Chemical Papers volume 70, pages 1460–1470 (2016)Cite this article

Abstract

Surfactant-stable keratinases with good properties are promising candidates for extensive applications in detergent industries. A novel fungal keratinase-producing strain, Gibberella intermedia CA3-1, is described in this study. The keratinase production medium was optimized and composed of 10 g L−1 of wool powder, 5 g L−1 of tryptone, 10 g L−1 of maltodextrin and 0.5 g L−1 of NaCl. Keratinase activity was increased up to 109 U mL−1 from 15 U mL−1 by culture optimization. The optimal reaction pH and temperature of the enzyme were 9.0 and 60°C, respectively. The keratinase activity could be improved by sodium dodecyl sulphate (SDS), and it remained stable in the presence of several surfactants and commercial detergents. G. intermedia keratinase was proved to completely remove blood stains from cotton cloth when combined with detergents. These findings indicate that this fungal keratinase is a promising catalyst for the application in detergent industry. To our knowledge, this is the first report on keratinase production by Gibberella genus.

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Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, China

    Rong-Xian Zhang, Jin-Song Gong, Wen-Fang Dou, Dan-Dan Zhang, Yu-Xia Zhang, Heng Li, Zhen-Ming Lu, Jin-Song Shi & Zheng-Hong Xu

  2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Rong-Xian Zhang & Zheng-Hong Xu

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  1. Rong-Xian Zhang
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  2. Jin-Song Gong
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Correspondence to Jin-Song Shi or Zheng-Hong Xu.

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Zhang, RX., Gong, JS., Dou, WF. et al. Production and characterization of surfactant-stable fungal keratinase from Gibberella intermedia CA3-1 with application potential in detergent industry. Chem. Pap. 70, 1460–1470 (2016). https://doi.org/10.1515/chempap-2016-0086

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  • DOI: https://doi.org/10.1515/chempap-2016-0086

Keywords

  • Gibberella intermedia
  • keratinase
  • detergents
  • surfactants
  • biocatalysis