Abstract
Phyllanthus amarus is known as a healing herb which has traditionally been used in the treatment of various diseases such as hepatitis, diabetes and cancer. The extraction parameters have great effects on the extraction efficiency of bioactive compounds and pharmacological activity of the extracts. This study sought to optimise the microwave-assisted extraction parameters for phenolic compounds-enriched extracts and antioxidant capacity from P. amarus using response surface methodology (RSM). The results showed that the optimal microwave-assisted extraction parameters were an extraction time of 30 min, an irradiation time of 14 s min−1 and a ratio of solvent to sample of 150 mL g−1. The total phenolic content, phenolic extraction efficiency, saponin content, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity, 2,2-diphenyl-1-picryl-hydrazil (DPPH) radical scavenging capacity and ferric reducing antioxidant power of the P. amarus achieved under these optimal parameters were 87.3 mg of gallic acid equivalents (GAE) per gram of dried sample, 69.7 %, 134.9 mg of escin equivalents (EE) per gram of dried sample, 997.8, 604.7 and 437.3 all in mg of trolox equivalents (TE) per gram of dried sample, respectively, which were not significantly different from the predicted values (86.9 mg of GAE per gram of dried sample, 67.3 %, 123.5 mg of EE per gram of dried sample, 1013.3 mg of TE per gram of dried sample, 530.6 mg of TE per gram of dried sample and 423.5 mg of TE per gram of dried sample, respectively). Accordingly, the optimal microwave-assisted extraction parameters of 30 min, 14 s min−1 and 150 mL g−1 are recommended for the extraction of enriched phenolics from P. amarus for potential application in the nutraceutical and pharmaceutical industries.
Similar content being viewed by others
References
Association of Official Analytical Chemists (1998). Official methods of analysis (16th ed.). Washington, DC, USA: AOAC.
Bai, X. L., Yue, T. L., Yuan, Y. H., & Zhang, H. W. (2010). Optimization of microwave-assisted extraction of polyphenols from apple pomace using response surface methodology and HPLC analysis. Journal of Separation Science, 33, 3751–3758. DOI: 10.1002/jssc.201000430.
Bhuyan, D. J., Vuong, Q. V., Chalmers, A. C., Altena, I. A. V., Bowyer, M. C., & Scarlett, C. J. (2015). Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds. Industrial Crops and Products, 69, 290–299. DOI: 10.1016/j.indcrop.2015.02.044.
Cheok, C. Y., Salman, H. A. K., & Sulaiman, R. (2014). Extraction and quantification of saponins: a review. Food Research International, 59, 16–40. DOI: 10.1016/j.foodres.2014.01.057.
Dahmoune, F., Nayak, B., Moussi, K., Remini, H., & Madani, K. (2015). Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chemistry, 166, 585–595. DOI: 10.1016/j.foodchem.2014.06.066.
Hari Kuma, K. B., & Kuttan, R. (2004). Protective effect of an extract of Phyllanthus amarus against radiation-induced damage in mice. Journal of Radiation Research, 45, 133–139. DOI: 10.1269/jrr.45.133.
Kha, T. C., Nguyen, M. H., Phan, D. T., Roach, P. D., & Stathopoulos, C. E. (2013). Optimisation of microwave- assisted extraction of Gac oil at different hydraulic pressure, microwave and steaming conditions. International Journal of Food Science and Technology, 48, 1436–1444. DOI: 10.1111/ijfs.12109.
Kha, T. C., Nguyen, M. H., Roach, P. D., & Stathopoulos, C. E. (2014a). Microencapsulation of Gac oil by spray drying: optimization of wall material concentration and oil load using response surface methodology. Drying Technology, 32, 385–397. DOI: 10.1080/07373937.2013.829854.
Kha, T. C., Nguyen, M. H., Roach, P. D., & Stathopoulos, C. E. (2014b). Microencapsulation of Gac oil: optimisation of spray drying conditions using response surface methodology. Powder Technology, 264, 298–309. DOI: 10.1016/j.powtec.2014.05.053.
Kwon, J. H., Belanger, J. M. R., & Pare, J. R. J. (2003). Optimization of microwave-assisted extraction (MAP) for Ginseng components by response surface methodology. Journal of Agricultural and Food Chemistry, 51, 1807–1810. DOI: 10.1021/jf026068a.
Lim, Y. Y., & Murtijaya, J. (2007). Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT — Food Science and Technology, 40, 1664–1669. DOI: 10.1016/j.lwt.2006.12.013.
Londhe, J. S., Devasagayam, T. P. A., Foo, L. Y., & Ghaskadbi, S. S. (2008). Antioxidant activity of some polyphenol constituents of the medicinal plant Phyllanthus amarus Linn. RedoxReport, 13, 199–207. DOI: 10.1179/135100008x308984.
Londhe, J. S., Devasagayam, T. P. A., Foo, L. Y., Shastry, P., & Ghaskadbi, S. S. (2012). Geraniin and amariin, ellagitannins from Phyllanthus amarus, protect liver cells against ethanol induced cytotoxicity. Fitoterapia, 83, 1562–1568. DOI: 10.1016/j.fitote.2012.09.003.
Maity, S., Chatterjee, S., Variyar, P. S., Sharma, A., Adhikari, S., & Mazumder, S. (2013). Evaluation of antioxidant activity and characterization of phenolic constituents of Phyllanthus amarus root. Journal of Agricultural and Food Chemistry, 61, 3443–3450. DOI: 10.1021/jf3046686.
Nguyen, V. T. (2014). Mass proportion, proximate composition and effects of solvents and extraction parameters on pigment yield from cacao pod shell (Theobroma cacao L.). Journal of Food Processing and Preservation. DOI: 10.1111/jfpp.12360. (in press)
Nguyen, V. T., Vuong, Q. V., Bowyer, M. C., van Altena, I. A., & Scarlett, C. J. (2015a). Effects of different drying methods on bioactive compound yield and antioxidant capacity of Phyllanthus amarus. Drying Technology, 33, 1006–1017. DOI: 10.1080/07373937.2015.1013197.
Nguyen, V. T., Pham, H. N. T., Bowyer, M. C., van Altena, I. A., & Scarlett, C. J. (2015b). Evaluating the influence of solvents and novel extraction methods on bioactive compounds and antioxidant capacity from Phyllanthus amarus. Chemical Papers. (in press)
Nguyen, V. T., Bowyer, M. C., Vuong, Q. V., van Altena, I. A., & Scarlett, C. J. (2015c). Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) root as affected by various solvents and extraction methods. Industrial Crops and Products, 67, 192–200. DOI: 10.1016/j.indcrop.2015.01.051.
Nguyen, V. T., Vuong, Q. V., Bowyer, M. C., van Altena, I. A., & Scarlett, C. J. (2015d). Microware-assisted extraction for saponins and antioxidant capacity from Xao tam phan (Paramignya trimera) root. Journal of Food Processing and Preservation. (accepted)
Osbourn, A., Goss, R. J. M., & Field, R. A. (2011). The saponins — polar isoprenoids with important and diverse biological activities. Natural Product Report, 28, 1261–1268. DOI: 10.1039/c1np00015b.
Patel, J. R., Tripathi, P., Sharma, V., Chauhana, N. S., & Dixit, V. K. (2011). Phyllanthus amarus: ethnomedicinal uses, phytochemistry and pharmacology: a review. Journal of Ethnopharmarcology, 138, 286–313. DOI: 10.1016/j.jep.2011. 09.040.
Poh Hwa, T., Yoke Kqueen, C., Indu Bala, J., & Son, R. (2011). Bioprotective properties of three Malaysia Phyllanthus species: an investigation of the antioxidant and antimicrobial activities. International Food Research Journal, 18, 887–893.
Roengrit, T., Wannanon, P., Prasertsri, P., Kanpetta, Y., Sripanidkulchai, B. O., & Leelayuwat, N. (2014). Antioxidant and anti-nociceptive effects of Phyllanthus amarus on improving exercise recovery in sedentary men: a randomized crossover (double-blind) design. Journal of the International Society of Sports Nutrition, 11, 9. DOI: 10.1186/1550–2783 11–9.
Sarin, B., Verma, N., Martin, J. P., & Mohanty, A. (2014). An overview of important ethnomedicinal herbs of Phyllanthus species: present status and future Prospects. The Scientific World Journal, 2014, 839–172. DOI: 10.1155/2014/839172.
Sen, A., & Batra, A. (2013). The study of in vitro and in vivo antioxidant activity and total phenolic content of Phyllanthus amarus schum. & thonn.: a medicinally important plant. International Journal of Pharmacy and Pharmaceutical Sciences, 5, 942–947.
Shokunbi, O. S., & Odetola, A. A. (2008). Gastroprotective and antioxidant activities of Phyllanthus amarus extracts on absolute ethanol-induced ulcer in albino rats. Journal of Medicinal Plants Research, 2(10), 261–267.
Tan, S. P., Vuong, Q. V., Stathopoulos, C. E., Parks, S. E., & Roach, P. D. (2014). Optimized aqueous extraction of saponins from bitter melon for production of a saponin-enriched bitter melon powder. Journal of Food Science, 79, E1372–E1381. DOI: 10.1111/1750–3841.12514.
Tang, Y. Q., & Sekaran, S. D. (2011). Evaluation of Phyllanthus for its anti-cancer properties. In P. E. Spiess (Ed.), Prostate cancer — from bench to bedside (pp. 305–320). Rijeka, Croatia: In Tech. DOI: 10.5772/27296.
Tang, Y. Q., Jaganath, I., Manikam, R., & Sekaran, S. D. (2013). Phyllanthus suppresses prostate cancer cell, PC-3, proliferation and induces apoptosis through multiple signalling pathways (MAPKs, PI3K/Akt, NFkB, and hypoxia). Evidence-Based Complementary and Alternative Medicine, 2013, 609581. DOI: 10.1155/2013/609581.
Teng, H., Lee, W. Y., & Choi, Y. H. (2013). Optimization of microwave-assisted extraction for anthocyanins, polyphenols, and antioxidants from raspberry (Rubus coreanus Miq.) using response surface methodology. Journal of Separation Science, 36, 3107–3114. DOI: 10.1002/jssc.201300303.
Vuong, Q. V., Stathopoulos, C. E., Golding, J. B., Nguyen, M. H., & Roach, P. D. (2011). Optimum conditions for the water extraction of L-theanine from green tea. Journal of Separation Science, 34, 2468–2474. DOI: 10.1002/jssc.201100401.
Vuong, Q. V., Goldsmith, C. D., Dang, T. T., Nguyen, V. T., Bhuyan, D. J., Sadeqzadeh, E., Scarlett, C. J., & Bowyer, M. C. (2014a). Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant capacity from Euphorbia tirucalli using response surface methodology. Antioxidants, 3, 604–617. DOI: 10.3390/antiox3030604.
Vuong, Q. V., Nguyen, V. T., Thanh, D. T., Bhuyan, D. J., Goldsmith, C. D., Sadeqzadeh, E., Scarlett, C. J., & Bowyer, M. C. (2014b). Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology. Industrial Crops and Products, 63, 197–202. DOI: 10.1016/j.indcrop.2014.09.057.
Vuong, Q. V., Hirun, S., Chuen, T. L. K., Goldsmith, C. D., Munro, B., Bowyer, M. C., Chalmers, A. C., Sakoff, J. A., Phillips, P. A., & Scarlett, C. J. (2015). Physicochemical, antioxidant and anti-cancer activity of a Eucalyptus robusta (Sm.) leaf aqueous extract. Industrial Crops and Products, 64, 167–174. DOI: 10.1016/j.indcrop.2014.10.061.
Wen, Y., Chen, H., Zhou, X., Deng, Q., Zhao, Y., Zhao, C., & Gong, X. (2015). Optimization of microwave-assisted extraction and antioxidant activities of anthocyanins from blackberry using a response surface methodology. RSC Advances, 5, 19686–19695. DOI: 10.1039/c4ra16396f.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Van Nguyen, T., Bowyer, M.C., Van Altena, I.A. et al. Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity. Chem. Pap. 70, 713–725 (2016). https://doi.org/10.1515/chempap-2016-0009
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1515/chempap-2016-0009