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Terbufos sulfone aggravates kidney damage in STZ-induced diabetic rats

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Abstract

The consequences of chronic exposure of organophosphorus compounds (OPCs) on diabetic subjects have been seldom reported. The aim of the present study was to assess the impact of non-lethal dose of terbufos sulfone (TS), an organophosphate, on the kidney of non-diabetic and streptozotocin (STZ)- induced diabetic rats. The diabetogenic effect of TS was also examined. Male Wistar rats were treated for two weeks with 130 µg/kg body weight/day of TS. This dose was 1/20 of LD50 and produces less than 10% inhibition of acetylcholinesterase (AChE) in non-diabetic rats. No observable symptoms of poisoning were noted. Weight, glucose and in vivo red blood cell (RBC)-AChE were measured on day 7 and 15 of the study. Serum biochemistry, urine analysis, and transmission electron microscopy (TEM) of kidney were performed to assess the toxicity at the end of the 15-day study. Our results did not reveal diabetogenic effect of TS treatment. Blood glucose level was significantly increased and RBC-AChE activity was significantly decreased in diabetic-TS treated rats compared to untreated diabetic and normal control animals. TEM of kidney revealed structural damage to kidney, which was more severe in diabetic-TS treated rats compared to controls. Toxicity to kidney by TS was further confirmed by urine and blood biochemistry. Our results conclude that non-lethal dose of TS aggravates the nephrotoxicity in diabetic rats. Further studies with other OPCs are needed to be able to generalize the diabetogenic effect of TS.

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Abbreviations

AChE:

acetylcholinesterase

BUN:

blood urea nitrogen

BW:

body weight

DM:

diabetes mellitus

i.p.:

intraperitoneal

IPGTT:

intraperitoneal glucose tolerance test

LD50:

lethal dose that kills 50% of test sample

LDH:

lactate dehydrogenase

OPCs:

organophosphorus compounds

PCT:

proximal convoluted tubules

RBC:

red blood cell

STZ:

streptozotocin

TEM:

transmission electron microscopy

TS:

terbufos sulfone

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Authors and Affiliations

  1. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates

    Syed Muhammad Nurulain, Shreesh Ojha & Mohamed Shafiullah

  2. Department of Biosciences, COMSATS-Institute of Information Technology, Islamabad, Pakistan

    Syed Muhammad Nurulain & Tayyaba Yasmin

  3. Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates

    Javed Yasin

  4. Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates

    Tariq Saeed & Ernest Adeghate

Authors
  1. Syed Muhammad Nurulain
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  2. Shreesh Ojha
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  3. Mohamed Shafiullah
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  4. Javed Yasin
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  5. Tayyaba Yasmin
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  6. Tariq Saeed
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  7. Ernest Adeghate
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Correspondence to Ernest Adeghate.

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Nurulain, S.M., Ojha, S., Shafiullah, M. et al. Terbufos sulfone aggravates kidney damage in STZ-induced diabetic rats. Biologia 72, 946–953 (2017). https://doi.org/10.1515/biolog-2017-0106

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  • DOI: https://doi.org/10.1515/biolog-2017-0106

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