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Forensic application of EST-derived STR markers in opium poppy

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Abstract

Discrimination study in opium poppy (Papaver somniferum L.) genotypes has been performed by short tandem repeat (STR) markers derived from expressed sequence tags (ESTs) retrieved from the opium poppy DNA. Some of STRs originate from the gene sequences involved in the biosynthesis of morphine. Eight of twelve tested EST-derived STR markers generated polymorphism, 2–4 alleles were identified per locus, and the mean polymorphic information content value was 0.338. Seven cultivars developed in Slovakia, registered and released at present time for commercial cultivation were discriminated mutually. All the Slovak and Czech cultivars were also different from each other. EST-derived STR markers have shown a potential for discrimination of opium poppy genotypes cultivated for commercial production of either edible seeds or pharmacologically active alkaloids and could be applicable for the PCR-based forensic analyses.

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Abbreviations

AFLP:

amplified fragment length polymorphism

DI:

diversity index

EST:

expressed sequence tag

HE:

expected heterozygosity

HO:

observed heterozygosity

PCA:

principal component analysis

PI:

probability of identity

PIC:

polymorphic information content

SSR:

simple sequence repeat

STR:

short tandem repeat

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Authors and Affiliations

  1. Department of Botany and Genetics, Faculty of Natural Sciences, Constantine The Philosopher University, Tr. A. Hlinku 1, SK-94974, Nitra, Slovakia

    Veronika Mičianová

  2. Research Institute of Plant Production, National Agricultural and Food Centre, Bratislavská cesta 122, SK-92168, Piešťany, Slovakia

    Katarína Ondreičková, Darina Muchová, Lenka Klčová, Martina Hudcovicová, Michaela Havrlentová, Daniel Mihálik & Ján Kraic

  3. Department of Biotechnology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, Nám. J. Herdu 2, SK-91701, Trnava, Slovakia

    Michaela Havrlentová, Daniel Mihálik & Ján Kraic

Authors
  1. Veronika Mičianová
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  2. Katarína Ondreičková
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  3. Darina Muchová
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  4. Lenka Klčová
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  5. Martina Hudcovicová
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  6. Michaela Havrlentová
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  7. Daniel Mihálik
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  8. Ján Kraic
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Correspondence to Ján Kraic.

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Mičianová, V., Ondreičková, K., Muchová, D. et al. Forensic application of EST-derived STR markers in opium poppy. Biologia 72, 587–594 (2017). https://doi.org/10.1515/biolog-2017-0076

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  • DOI: https://doi.org/10.1515/biolog-2017-0076

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