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Role of FIT2 in porcine intramuscular preadipocyte differentiation

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Abstract

Fat-inducing transcript 2 (FIT2) plays an important role in the formation of intramuscular lipid droplets in skeletal muscle. However, its role in porcine intramuscular preadipocyte differentiation remains unclear. In the present study, a 789-bp fragment covering the complete coding region of porcine FIT2 (pFIT2) was obtained. Real-time quantitative PCR analysis indicated that pFIT2 mRNA was highly expressed in fat tissue. Overexpression of pFIT2 in porcine intramuscular preadipocytes led to an increase in lipid accumulation, which was detected by triglyceride content analysis. Overexpression of pFIT2 also significantly increased the protein expressions of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer binding protein-α (C/EBPα), but decreased the protein expression of β-catenin. We also found that the Wnt/β-catenin signaling specific activator LiCl attenuated the pFIT2-induced upregulation of PPARPPARγ and downregulation of β-catenin. These findings implied that FIT2 promotes porcine intramuscular preadipocyte differentiation by repressing Wnt/β-catenin signaling.

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Abbreviations

DMEM:

Dulbecco’s modified Eagle medium

DLY:

Duroc × Landrace × Yorkshire

ER:

endoplasmic reticulum

FBS:

fetal bovine serum

FIT2:

fat-inducing transcript 2

FITM2:

fat storage-inducing transmembrane protein 2

IMF:

intramuscular fat

LD:

lipid droplet

LL:

longissimus lumborum

pFIT2 :

porcine FIT2

PM:

psoas muscle

PPARγ:

peroxisome proliferator-activated receptor γ

TG:

triglyceride

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31472108) and the Specific Research Supporting Program for Discipline Construction in Sichuan Agricultural University.

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Correspondence to Zhiqing Huang.

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Chen, X., Luo, Y., Jia, G. et al. Role of FIT2 in porcine intramuscular preadipocyte differentiation. Biologia 71, 1404–1409 (2016). https://doi.org/10.1515/biolog-2016-0169

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