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Role of FIT2 in porcine intramuscular preadipocyte differentiation

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Fat-inducing transcript 2 (FIT2) plays an important role in the formation of intramuscular lipid droplets in skeletal muscle. However, its role in porcine intramuscular preadipocyte differentiation remains unclear. In the present study, a 789-bp fragment covering the complete coding region of porcine FIT2 (pFIT2) was obtained. Real-time quantitative PCR analysis indicated that pFIT2 mRNA was highly expressed in fat tissue. Overexpression of pFIT2 in porcine intramuscular preadipocytes led to an increase in lipid accumulation, which was detected by triglyceride content analysis. Overexpression of pFIT2 also significantly increased the protein expressions of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT-enhancer binding protein-α (C/EBPα), but decreased the protein expression of β-catenin. We also found that the Wnt/β-catenin signaling specific activator LiCl attenuated the pFIT2-induced upregulation of PPARPPARγ and downregulation of β-catenin. These findings implied that FIT2 promotes porcine intramuscular preadipocyte differentiation by repressing Wnt/β-catenin signaling.

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Dulbecco’s modified Eagle medium


Duroc × Landrace × Yorkshire


endoplasmic reticulum


fetal bovine serum


fat-inducing transcript 2


fat storage-inducing transmembrane protein 2


intramuscular fat


lipid droplet


longissimus lumborum

pFIT2 :

porcine FIT2


psoas muscle


peroxisome proliferator-activated receptor γ




  • Chen X.L., Zhou B., Huang Z.Q., Jia G., Liu G.M. & Zhao H. 2016. Tissue distribution of porcine FTO and its effect on porcine intramuscular preadipocytes proliferation and differentiation. PLoS One 11: e0151056.

    Article  Google Scholar 

  • Clark K., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Sayers E.W. 2016. GenBank. Nucleic Acids Res. 44: D67–D72.

    Article  CAS  Google Scholar 

  • Gao S.Z. & Zhao S.M. 2009. Physiology, affecting factors and strategies for control of pig meat intramuscular fat. Recent Pat. Food. Nutr. Agric. 1: 59–74.

    Article  CAS  Google Scholar 

  • Gardan D., Gondret F. & Louveau I. 2006. Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes. Am. J. Physiol. Endocrinol. Metab. 291: E372–E380.

    Article  CAS  Google Scholar 

  • Gross D.A., Snapp E.L. & Silver D.L. 2010. Structural insights into triglyceride storage mediated by fat storage-inducing transmembrane (fit) protein 2. PLoS One 5: e10796.

    Article  Google Scholar 

  • Gross D.A., Zhan C. & Silver D.L. 2011. Direct binding of triglyceride to fat storage-inducing transmembrane proteins 1 and 2 is important for lipid droplet formation. Proc. Natl. Acad. Sci. USA 108: 19581–19586.

    Article  CAS  Google Scholar 

  • Kadereit B., Kumar P., Wang W.J., Miranda D., Snapp E.L., Severina N., Torregroza I., Evans T. & Silver D.L. 2008. Evolutionary conserved gene family important for fat storage. Proc. Natl. Acad. Sci. USA 105: 94–99.

    Article  CAS  Google Scholar 

  • Kennell J.A. & MacDougald O.A. 2005. Wnt signaling inhibits adipogenesis through β-catenin-dependent and -independent mechanisms. J. Biol. Chem. 280: 24004–24010.

    Article  CAS  Google Scholar 

  • Kim M.B., Song Y., Kim C. & Hwang J.K. 2014. Kirenol inhibits adipogenesis through activation of the Wnt/β-catenin signaling pathway in 3T3-L1 adipocytes. Biochem. Biophys. Res. Commun. 445: 433–438.

    Article  CAS  Google Scholar 

  • Li C. & Zhou L. 2015. Inhibitory effect 6-gingerol on adipogenesis through activation of the Wnt/β-catenin signaling pathway in 3T3-L1 adipocytes. Toxicol. In Vitro 30: 394–401.

    Article  CAS  Google Scholar 

  • Martin S. & Parton R.G. 2006. Lipid droplets: a unified view of a dynamic organelle. Nat. Rev. Mol. Cell Biol. 7: 373e378.

    Article  Google Scholar 

  • Miranda D.A., Kim J.H., Nguyen L.N., Cheng W., Tan B.C., Goh V.J., Tan J.S.Y., Yaligar J., KN B.P., Velan S.S., Wang H. & Silver D.L. 2014. Fat storage-inducing transmembrane protein 2 is required for normal fat storage in adipose tissue. J. Biol. Chem. 289: 9560–9572.

    Article  CAS  Google Scholar 

  • Miranda D.A., Koves T.R., Gross D.A., Chadt A., Al-Hasani H., Cline G.W., Schwartz G.J., Muoio D.M. & Silver D.L. 2011. Re-patterning of skeletal muscle energy metabolism by fat storage-inducing transmembrane protein 2. J. Biol. Chem. 286: 42188–42199.

    Article  CAS  Google Scholar 

  • Nguyen L.N., Hamari Z., Kadereit B., Trofa D., Agovino M., Martinez L.R., Gacser A., Silver D.L. & Nosanchuk J.D. 2011. Candida parapsilosis fat storage-inducing transmembrane (FIT) protein 2 regulates lipid droplet formation and impacts virulence. Microbes Infect. 13: 663–672.

    Article  CAS  Google Scholar 

  • Pang W.J., Bai L. & Yang G.S. 2006. Relationship among H-FABP gene polymorphism, intramuscular fat content, and adipocyte lipid droplet content in main pig breeds with different genotypes in western China. Yi Chuan Xu Bao - Acta Genetica Sinica 33: 515–524.

    Article  CAS  Google Scholar 

  • Prestwich T.C. & MacDougald O.A. 2007. Wnt/β-catenin signaling in adipogenesis and metabolism. Curr. Opin. Cell Biol. 19: 612–617.

    Article  CAS  Google Scholar 

  • Rosen E.D., Sarraf P., Troy A.E., Bradwin G., Moore K., Mil-stone D.S., Spiegelman B.M. & Mortensen R.M. 1999. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol. Cell 4: 611–617.

    Article  CAS  Google Scholar 

  • Ross S.E., Hemati N., Longo K.A., Bennett C.N., Lucas P.C., Erickson R.L. & MacDougald O.A. 2013. Inhibition of adipogenesis by Wnt signaling. Science 289: 950–953.

    Article  Google Scholar 

  • Wahli W. & Michalik L. 2012. PPARs at the crossroads of lipid signaling and inflammation. Trends Endocrinol. Metab. 23: 351–363.

    Article  CAS  Google Scholar 

  • Walther T.C, Farese R.V & Jr. 2012. Lipid droplets and cellular lipid metabolism. Annu. Rev. Biochem. 81: 687–714.

    Article  CAS  Google Scholar 

  • Willson T.M., Brown P.J., Sternbach D.D. & Henke B.R. 2000. The PPARs: from orphan receptors to drug discovery. J. Med. Chem. 43: 527–550.

    Article  CAS  Google Scholar 

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This work was supported by the National Natural Science Foundation of China (No. 31472108) and the Specific Research Supporting Program for Discipline Construction in Sichuan Agricultural University.

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Correspondence to Zhiqing Huang.

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Chen, X., Luo, Y., Jia, G. et al. Role of FIT2 in porcine intramuscular preadipocyte differentiation. Biologia 71, 1404–1409 (2016).

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