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Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper

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Abstract

Media components were optimized using two-step statistical design of experiments for enhancing endoglucanase/carboxymethyl cellulase (CMCase) production by Trichoderma reesei RUT C30. A Placket-Burman design identified cellulose concentration and pH as the most significant variables, which influenced the CMCase activity. Central composite design was employed to optimize these selected parameters. The optimal activity was obtained at cellulose concentration 19.7 g/L and pH of 7.2. Under the optimized conditions, CMCase activity was 83.63 ± 1.86 IU/mL and filter paper activity was 2.58 ± 0.2 filter paper units per mL. Enzyme productivity was higher compared to previous reports. The enzyme produced from T. reesei was concentrated and was evaluated for deinking of printed paper, which demonstrated the suitability of the enzyme for this application.

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Abbreviations

ANOVA:

analysis of variance

CCD:

central composite design

CMC:

carboxymethyl cellulose

CMCase:

carboxymethyl cellulase

CSL:

corn steep liquor

DAHP:

diammonium hydrogenphosphate

DNS:

3′5′-dinitrosalicylic acid

IU:

international unit

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Acknowledgements

ASOI gratefully acknowledges the financial assistance provided by Department of Biotechnology (DBT), Government of India, and The World Academy of Sciences for the Advancement of Science in developing countries (TWAS) in the form of fellowship for supporting his PhD program, of which this study forms a part.

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Authors and Affiliations

  1. Centre for Biofuels, Biotechnology Division, CSIR - National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram, 695019, India

    Ayman Salih Omer Idris, Ashok Pandey & Rajeev Kumar Sukumaran

  2. Academy of Scientific and Innovative Research, New Delhi, 110025, India

    Ayman Salih Omer Idris

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  1. Ayman Salih Omer Idris
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  2. Ashok Pandey
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  3. Rajeev Kumar Sukumaran
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Correspondence to Rajeev Kumar Sukumaran.

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Idris, A.S.O., Pandey, A. & Sukumaran, R.K. Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper. Biologia 71, 265–271 (2016). https://doi.org/10.1515/biolog-2016-0046

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  • DOI: https://doi.org/10.1515/biolog-2016-0046

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