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Inhibitory effect of Murraya koenigii against Candida albicans virulence and biofilm development

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Abstract

In this study, anti-infective potential of the medicinal plant Murraya koenigii was assessed through in vitro assays and microscopic analysis. The methanolic leaf extract of M. koenigii significantly inhibited the major virulence factors of Candida albicans, such as biofilm formation, yeast-to-hyphal transition, cell surface hydrophobicity, hemolysin production and filamentation. Further purification and molecular characterization of the active lead is expected to give a novel anticandidal agent for the treatment of Candida infection.

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Abbreviations

CLSM:

confocal laser scanning microscopy

MBIC:

minimum biofilm inhibitory concentration

MKM:

Murraya koenigii methanolic

MTP:

microtiter plate

PBS:

phosphate buffered saline

SDA:

Sabouraud dextrose agar

SEM:

scanning electron microscopy

XTT:

2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide

YEPD:

yeast extract peptone dextrose

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Acknowledgements

The authors thankfully acknowledge the Bioinformatics Infrastructure Facility funded by Department of Biotechnology, Government of India [Grant No. BT/BI/25/015/2012 (BIF)], the instrumentation facility provided by Department of Science and Technology, Government of India through PURSE [Grant No. SR/S9Z-23/2010/42 (G)] & FIST (Grant No. SR-FST/LSI-087/2008), and University Grants Commission (UGC), New Delhi, through SAP-DRS1 [Grant No. F.3-28/2011 (SAP-II)]. SM thanks UGC for financial assistance in the form of a Basic Scientific Research Fellowship [Sanction No. F.25-1/2013-14 (BSR)/7-326/2011 (BSR) dt 30.05.2014].

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  1. Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, 630 004, Tamil Nadu, India

    Subramanian Muthamil & Shunmugiah Karutha Pandian

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  1. Subramanian Muthamil
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Correspondence to Shunmugiah Karutha Pandian.

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Muthamil, S., Pandian, S.K. Inhibitory effect of Murraya koenigii against Candida albicans virulence and biofilm development. Biologia 71, 256–264 (2016). https://doi.org/10.1515/biolog-2016-0044

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