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Me11b receptor mediated action of melatonin in regulation of lung associated immune system (LAIS) of Perdicula asiatica: An in vitro study

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Abstract

The pineal hormone melatonin plays an important role in immunomodulation of avian species. These immunomodulatory effects of melatonin are known to be mediated by three membrane bound receptors found in birds, Mel1a, Mel1b and Mel1c. However, specific involvement of these receptors in mediating immunomodulatory effects of melatonin in Lung Associated Immune System (LAIS) remains unexplored till date. The aim of the present study was to check the specific melatonin receptor subtype involved in regulation of LAIS. For this purpose, we used luzindole, a non selective and 4P-PDOT, a selective Mel1b receptor antagonist under in vitro condition to assess their specific potency towards melatonin induced cellular immunity in the lung tissue of Perdicula asiatica. Melatonin enhanced proliferation of lymphocytes isolated from lung tissue and up-regulated both receptor types (Mel1a and Mel1b) on physiological doses while higher doses decreased the proliferation and down regulated the expression of both receptors. Luzindole decreased proliferation of lung lymphocytes and down-regulated the expression of both receptors in a dose dependent manner while 4P-PDOT decreased proliferation of lymphocytes isolated from lung and down-regulated the expression of Mel1b receptor subtype only. Proliferation rate of lymphocytes isolated from lung tissue followed more the expression pattern of Mel1b than the Mel1a. Thus, we may suggest that Mel1b might be involved in cell mediated immunity in lung of P. asiatica to a greater extent than Mel1a.

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Acknowledgements

Instruments (CO2 incubator and high speed refrigerated centrifuge) from Alexander von Humboldt Foundation, Germany and financial support from Council for Scientific and Industrial Research, New Delhi to Rajesh Kumar Kharwar is greatly acknowledged.

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Authors and Affiliations

  1. Department of Zoology, Kutir Post Graduate College, Chakkey, Jaunpur, 222 146, Uttar Pradesh, India

    Rajesh Kumar Kharwar & Sweta Singh

  2. Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, India

    Chandana Haldar

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  1. Rajesh Kumar Kharwar
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  2. Chandana Haldar
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  3. Sweta Singh
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Correspondence to Rajesh Kumar Kharwar.

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Kharwar, R.K., Haldar, C. & Singh, S. Me11b receptor mediated action of melatonin in regulation of lung associated immune system (LAIS) of Perdicula asiatica: An in vitro study. Biologia 70, 1537–1544 (2015). https://doi.org/10.1515/biolog-2015-0177

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  • DOI: https://doi.org/10.1515/biolog-2015-0177

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