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Transcriptome analysis for identification of indigo biosynthesis pathway genes in Polygonum tinctorium

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Abstract

Indigo is the most important blue dye for textile dyeing and is biosynthesized in Polygonum tinctorium. Some biochemical studies related to biosynthesis are available. However, genomic and transcriptome studies have not received sufficient attention. Here, we report de novo assembly of transcriptome datasets and its comprehensive analysis. A total of 60,395 unigenes were annotated using BLAST search against the different databases. At least 23,721 unigenes mapped onto different pathways using KEGG database. We found that 3,323 genes are involved in biosynthesis of secondary metabolites, 117 phenylalanine, tyrosine and tryptophan biosynthesis and 147 tryptophan metabolisms. Apart from this, indigo biosynthesis pathway genes viz., dioxygenase, monooxygenase, and glucosyltransferase have also been identified. Fourteen genes encoding cytochrome P450 monooxygenase, 26 glucoside dioxygenase, 9 UDP-glucose D-glucosyltransferase and 52 were β-D-glucosidase. These findings provide a foundation for further analysis of this pathway with potential to enhance the synthesis of indican in P. tinctorium.

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Abbreviations

COG:

clusters of orthologous group

GO:

gene ontology

KEGG:

Kyoto Encyclopedia of Genes and Genomes

NR:

non-redundant protein database

NT:

non-redundant nucleotide database

UDP:

uridine diphosphate glucose

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Authors and Affiliations

  1. Department of Biochemistry, Okayama University of Science, Japan

    Yoshiko Minami

  2. Environmental Biotechnology Division, CSIR - National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India

    Bijaya Ketan Sarangi & Sanjog Tarachand Thul

Authors
  1. Yoshiko Minami
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  2. Bijaya Ketan Sarangi
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  3. Sanjog Tarachand Thul
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Correspondence to Sanjog Tarachand Thul.

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Minami, Y., Sarangi, B.K. & Thul, S.T. Transcriptome analysis for identification of indigo biosynthesis pathway genes in Polygonum tinctorium. Biologia 70, 1026–1032 (2015). https://doi.org/10.1515/biolog-2015-0131

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  • DOI: https://doi.org/10.1515/biolog-2015-0131

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