Abstract
B-cell lymphoma 2 (Bcl-2) and heat shock protein (HSP) families are implicated in various processes of carcinogenesis, owing to their role in regulation of apoptosis and cell cycle, respectively. mRNA expression of Bcl-2, myeloid cell leukemia 1 (Mcl-1), B-cell lymphoma-extra-large (Bcl-xl), Bcl2-associated X (Bax), HSP70 and HSP90-β genes were studied in 30 clinical canine mammary tumors (CMTs). Histological ‘type’ and ‘grade’ were assigned to CMTs and expression was evaluated by SYBR-Green real-time PCR assay. Overall, the tumors exhibited the maximum expression of Bcl-2 amongst the Bcl-2 family members. Sarcoma and carcinosarcoma showed relatively higher expression of Mcl-1, whereas Bcl-2 was over-expressed in carcinoma. In relation to the cancer grades, Bcl-2/Bax ratio tend to increase as the tumor differentiation progressed from well to poorly differentiated. HSP90-β exhibited significantly high expression in carcinoma, carcinosarcoma and all grades of CMTs were suggestive of their elemental role in tumor progression. In conclusion, this study underpins the conjecture that Bcl-2, Mcl-1 and HSP90-β can be used as potential targets of inhibition in future mammary tumor therapeutics.
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Abbreviations
- Bax:
-
Bcl2-associated X
- Bcl-2:
-
B-cell lymphoma 2
- Bcl-xl:
-
B-cell lymphoma-extra-large
- CMT:
-
canine mammary tumor
- Ct:
-
cycle threshold
- HSP:
-
heat-shock protein
- Mcl-1:
-
myeloid cell leukemia 1
- OM:
-
original magnification
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Acknowledgements
The authors are grateful to the Director, School of Animal Biotechnology, Dean, Post Graduate Studies and Director of Research, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana (Punjab), India, for providing funds and necessary infrastructural facilities to carry out this study.
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Mitra, N., Verma, R., Deka, D. et al. Differential expression of apoptosis-associated genes in canine mammary tumors. Biologia 70, 846–852 (2015). https://doi.org/10.1515/biolog-2015-0094
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DOI: https://doi.org/10.1515/biolog-2015-0094