Abstract
A total of 152 halophilic bacteria were isolated from Yuncheng Salt Lake, China. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that thirty-four strains were related to the phylum Firmicutes and belonged to three families, Bacillaceae, Clostridiaceae and Staphylococcaceae. The other strains were identified as the members of Halomonadaceae and Idiomarinaceae, which belonged to the phylum γ-Proteobacteria. Nine strains showed <97% similarity of 16S rRNA gene sequence compared to other published species, which might represent novel species. The halophilic isolates exhibited various hydrolytic activities. A total of 74, 15, 70, 18, 23 and 3 strains were found to produce extracellular amylase, protease, lipase, cellulase, pectinase and DNAase, respectively. Most hydrolase-producers were members of the genus Halomonas. Combined hydrolytic activities were shown by some strains. Screening of antimicrobial activity indicated that 3, 6, 15, 12, 15 and 16 of halophilic isolates could inhibit Staphylococcus aureus, Escherichia coli, Candida albicans, Fusarium moniliforme, Fusarium semitectum and Fusarium oxysporum, respectively. Results from the present study indicated that halophilic bacteria may be developed as promising sources for novel biocatalysts or bioactive substances in the field of biotechnology.
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Abbreviations
- CM:
-
complex medium
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Acknowledgements
This work was financially supported by National Natural Science Foundation of China (grant No. 31300002), Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi (2015) and PhD Start-up Foundation of Yuncheng University (grant No. YQ-2011043).
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Li, X., Yu, Y.H. Biodiversity and screening of halophilic bacteria with hydrolytic and antimicrobial activities from Yuncheng Salt Lake, China. Biologia 70, 151–156 (2015). https://doi.org/10.1515/biolog-2015-0033
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DOI: https://doi.org/10.1515/biolog-2015-0033