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A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis

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Abstract

The veterinary important parasite of ruminants, giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), isolated from liver of farmed fallow deer (Dama dama) from Podkarpackie Province (southeastern Poland) was genotypized by mitochondrial cytochrome c oxidase (cox1) and nicotinamide dehydrogenase (nad1) markers. The data on this newly emerged population were compared with mitochondrial haplotypes of recently detected Polish population of F. magna from Lower Silesian Wilderness (southwestern Poland) and with European populations of the parasite from all three natural foci; northern Italy, Czech Republic and the Danube floodplain forests. The flukes from Podkarpackie Province were found to be genetically identical with flukes from Czech Republic and Lower Silesian Wilderness in Poland. It is evident that central and southwestern Czech Republic, recognized as one of the endemic area of F. magna in Europe, has been enlarging and parasite has been invading several novel localities in Poland.

Note: Nucleotide sequence data reported in this paper are available in the GenBankTM, EMBL and DDBJ databases under the accession numbers KX279954 - 5

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References

  • Apostolo C. 1996. The naturalistic aspects: flora, fauna and the environment. In: (Eds M. Lupo, M. Paglieri, C. Apostolo, E. Vaccarino and M. Debernardi) La Mandria Storia e natura del Parco. Edizioni Eda, Torino, 67–98

    Google Scholar 

  • Bazsalovicsová E., Králová-Hromadová I., Spakulová M., Reblánová M., Oberhauserová K. 2010. Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia, 47, 76–82. DOI: 10.2478/s11687-010-0011-1

    Article  Google Scholar 

  • Bazsalovicsová E., Králová-Hromadová I., Štefka J., Minárik G., Bokorová S., Pybus M. 2015. Genetic interrelationships of North American populations of giant liver fluke Fascioloides magna. Parasites & Vectors, 8, 288. DOI: 10.1186/s13071015-0895-1

    Article  Google Scholar 

  • Demiaszkiewicz A.W., Kuligowska I., Pyziel A.M., Lachowicz J., Kowalczyk R. 2015. Extension of occurrence area of the American fluke Fascioloides magna in south-western Poland. Annals of Parasitology, 61, 93–96

    PubMed  Google Scholar 

  • Demiaszkiewicz A.W., Pyziel A.M., Kuligowska I., Lachowicz J. 2016. Fascioloides magna pasozytem jeleni w Borach Dolnoslqskich. Medycyna Weterynaryjna, 72, 110–112. (In Polish)

    Google Scholar 

  • Foreyt W.J., Todd A.C. 1976. The development of the large American liver fluke, Fascioloides magna, in white-tailed deer, cattle, and sheep. Journal of Parasitology, 62, 26–32. DOI: 10.2307/3279036

    Article  CAS  Google Scholar 

  • Karamon J., Larska M., Jasik A., Sell B. 2015. First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland — pathomorphological changes and molecular identification. Bulletin of the Veterinary Institute in Pulawy, 59, 339–344. DOI:10.1515/bvip2015-0050

    Article  Google Scholar 

  • Kašny M., Beran L., Siegelová V., Siegel T., Leontovyc R., Beránková K., Pankrác J., Košt’áková M., Horák P. 2012. Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Veterinarni Medicina, 57, 101–109

    Article  Google Scholar 

  • Králová-Hromadová I., Spakulová M., Horácková E., Turceková L., Novobilsky A., Beck R., Koudela B., Marinculic A., Rajsky D., Pybus M. 2008. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology, 94, 58–67. DOI:10.1645/GE-1324.1

    Article  Google Scholar 

  • Králová-Hromadová I., Bazsalovicsová E., Štefka J., Spakulová M., Vávrová S., Szemes T., Tkach V., Pybus M. 2011. Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. International Journal for Parasitology, 41, 373–383. DOI: 10.1016/j.ijpara.2010.10.010

    Article  Google Scholar 

  • Králová-Hromadová I., Bazsalovicsová E., Demiaszkiewicz A.W. 2015. Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers. Acta Parasitologica, 60, 544–547. DOI: 10.1515/ap-2015-0077

    Article  Google Scholar 

  • Králová-Hromadová I., Juhásová E., Bazsalovicsová E. (Eds). 2016. Giant liver fluke, Fascioloides magna: past, present and future research. Springer International Publishing, Switzerland Springer Briefs in Animal Sciences, pp. 106 DOI: 10.1007/978-3-319-29508-4

    Google Scholar 

  • Novobilsky A., Horácková E., Hirtová L., Modry D., Koudela B. 2007. The giant liver fluke Fascioloides magna (Bassi 1875) in cervids in the Czech Republic and potential of its spreading to Germany. Parasitology Research, 100, 549–553. DOI: 10.1007/s00436-006-0299-4

    Article  Google Scholar 

  • Plötz C., Rehbein S., Bamler H., Reindl H., Pfister K., Scheuerle M.C. 2015. Fascioloides magna — epizootiology in a deer farm in Germany. Berliner und Münchener tierärztliche Wochenschrift, 128, 177–182. DOI: 10.2376/0005-9366-128-177

    PubMed  Google Scholar 

  • Pybus M.J. 2001. Liver flukes. In: (Eds W.M. Samuel, M.J. Pybus and A.A. Kocan) Parasitic diseases of wild mammals. Edn. 2. Iowa State University Press, Ames, 121–149

    Chapter  Google Scholar 

  • Pyziel A.M., Demiaszkiewicz A.W., Kuligowska I. 2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from south-western Poland (Lower Silesian Wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523–525. DOI: 10.2478/pjvs-2014-0077

    Article  CAS  Google Scholar 

  • Rehbein S., Hamel D., Reindl H., Visser M., Pfister K. 2012. Fascioloides magna and Ashworthius sidemi — two new parasites in wild ungulates in Germany. In: Program and Abstracts of the XI European Multicolloquium of Parasitology (EMOP XI), 25–29 July, 2012, Cluj-Napoca, Romania, 565

    Google Scholar 

  • Salomon S. (1932) Fascioloides magna bei deutschem Rotwild. Berliner und Münchener tierärztliche Wochenschrift, 48, 627–628. (In German)

    Google Scholar 

  • Slusarski W. 1955. Studia nad europejskimi przedstawicielami przywry Fasciola magna (Bassi, 1875) Stiles, 1894. Acta Parasitologica Polonica, 3, 1–59. (In Polish)

    Google Scholar 

  • Swales W.E. 1935. The life cycle of Fascioloides magna (Bassi, 1875), the large liver fluke of ruminants, in Canada. Canadian Journal of Research, 12, 177–215. DOI: 10.1139/cjr35 015

    Article  Google Scholar 

  • Ullrich K. 1930. Über das Vorkommen von seltenen oder wenig bekannten Parasiten der Säugetiere und Vögel in Böhmen und Mähren. Prag Arch Tiermed, 10, 19–43. (In German)

    Google Scholar 

  • Werle E., Schneider C., Renner M., Volker M., Fiehn W. 1994. Convenient single-step, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354–4355

    Article  CAS  Google Scholar 

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Correspondence to Ivica Králová-Hromadová.

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Juhásová, Ľ., Bazsalovicsová, E., Králová-Hromadová, I. et al. A genetic structure of novel population of Fascioloides magna from Poland, Podkarpackie Province, indicates an expanding second European natural focus of fascioloidosis. Acta Parasit. 61, 790–795 (2016). https://doi.org/10.1515/ap-2016-0109

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  • DOI: https://doi.org/10.1515/ap-2016-0109

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